matrix protein M (NC_001563) Virus Tagged ORF Clone

CAT#: VC102505

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, NP_776013

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: matrix protein M
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102505 represents NCBI reference of NP_776013 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTTACCGTGCAGACGCACGGAGAGTCAACCCTGGCTAATAAGAAGGGGGCCTGGCTCGACTCTA
  CCAAAGCCACAAGATACCTGGTGAAGACCGAAAGTTGGATTCTGCGGAACCCCGGCTACGCCCTGGTCGC
  AGCGGTGATCGGCTGGATGCTCGGATCTAATACCATGCAGAGAGTGGTGTTCGCGATCCTCCTCCTCCTC
  GTAGCCCCAGCTTATAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102505 representing NP_776013
  Red=Cloning sites Green=Tags
  
  MSLTVQTHGESTLANKKGAWLDSTKATRYLVKTESWILRNPGYALVAAVIGWMLGSNTMQRVVFAILLLL
  VAPAYS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001563
• ORF Size: 228 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001563.2, NP_776013
• RefSeq ORF: 228
• MW: 8.3 kDa