non-structural protein NS2a (NC_001563) Virus Tagged ORF Clone

CAT#: VC102508

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, NP_776016

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: non-structural protein NS2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102508 represents NCBI reference of NP_776016 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACAATGCAGATATGATTGATCCGTTCCAGCTGGGGCTGATGGTGGTTTTCCTCGCAACCCAGGAAG
  TCCTTCGAAAGAGATGGACGGCGAAGATCAGTATCCCGGCAATTATGTTGGCATTGCTGGTACTGGTGTT
  TGGCGGCATCACTTACACTGACGTATTGAGATACGTGATTCTCGTCGGCGCAGCCTTCGCAGAGGCCAAC
  AGCGGTGGCGATGTTGTACACCTGGCCCTCATGGCCACTTTTAAAATCCAACCAGTGTTCCTGGTGGCTT
  CCTTCCTGAAAGCCAGATGGACCAACCAGGAAAGCATCCTGCTTATGTTGGCCGCCGCCTTCTTTCAAAT
  GGCCTATTACGATGCAAAAAATGTCCTTTCCTGGGAGGTGCCTGATGTACTCAACTCCCTTAGCGTGGCC
  TGGATGATACTTAGGGCTATCTCCTTCACTAACACTTCAAACGTCGTTGTCCCTCTGCTCGCCCTGCTGA
  CCCCAGGGCTCAAGTGCTTGAACCTCGACGTGTATCGAATACTGCTCCTTATGGTCGGAGTTGGCTCCCT
  GATTAAGGAGAAGCGCAGTTCCGCAGCGAAAAAGAAGGGCGCTTGCCTTATCTGTCTGGCACTTGCTTCC
  ACCGGAGTGTTCAACCCAATGATACTGGCGGCCGGATTGATGGCCTGCGATCCTAACCGCAAGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102508 representing NP_776016
  Red=Cloning sites Green=Tags
  
  MYNADMIDPFQLGLMVVFLATQEVLRKRWTAKISIPAIMLALLVLVFGGITYTDVLRYVILVGAAFAEAN
  SGGDVVHLALMATFKIQPVFLVASFLKARWTNQESILLMLAAAFFQMAYYDAKNVLSWEVPDVLNSLSVA
  WMILRAISFTNTSNVVVPLLALLTPGLKCLNLDVYRILLLMVGVGSLIKEKRSSAAKKKGACLICLALAS
  TGVFNPMILAAGLMACDPNRKR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001563
• ORF Size: 696 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001563.2, NP_776016
• RefSeq ORF: 696
• MW: 25.4 kDa