non-structural protein NS4a (NC_001563) Virus Tagged ORF Clone

CAT#: VC102511

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, NP_776019

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: non-structural protein NS4a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102511 represents NCBI reference of NP_776019 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAAATTGGCCTTGTGGAAGTTCTGGGCAGGATGCCAGAACATTTCATGGTTAAGACCTGGGAGG
  CTCTCGACACAATGTACGTCGTGGCTACCGCCGAGAAGGGGGGACGCGCCCACAGGATGGCTCTCGAGGA
  GCTGCCTGATGCACTCCAGACAATTGTGCTGATAGCCCTTCTGTCCGTGATGAGCCTCGGCGTATTCTTC
  CTGCTGATGCAGCGCAAGGGCATTGGTAAGATTGGCCTCGGTGGTGTGATACTGGGCGCAGCCACCTTCT
  TTTGCTGGATGGCAGAGGTCCCAGGTACCAAAATTGCCGGTATGCTCCTGCTGTCTCTCTTGCTGATGAT
  AGTGTTGATTCCTGAACCCGAGAAGCAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102511 representing NP_776019
  Red=Cloning sites Green=Tags
  
  MSQIGLVEVLGRMPEHFMVKTWEALDTMYVVATAEKGGRAHRMALEELPDALQTIVLIALLSVMSLGVFF
  LLMQRKGIGKIGLGGVILGAATFFCWMAEVPGTKIAGMLLLSLLLMIVLIPEPEKQR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001563
• ORF Size: 381 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001563.2, NP_776019
• RefSeq ORF: 381
• MW: 13.9 kDa