non-structural protein NS1 (NC_001563) Virus Tagged ORF Clone

CAT#: VC102507

Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [West Nile virus], codon optimized for human cell expression, NP_776015

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: non-structural protein NS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102507 represents NCBI reference of NP_776015 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACAGGGTGCGCGATCGATATCGGGCGACAGGAGCTGCGATGTGGGAGCGGCGTGTTTATCCATA
  ACGACGTTGAGGCATGGATGGATAGGTACAAATTCTACCCAGAAACCCCCCAGGGCCTGGCCAAGATCAT
  TCAGAAGGCTCACGCCGAAGGAGTGTGCGGTCTGCGGTCCGTCAGCAGGCTTGAACATCAGATGTGGGAA
  GCCATAAAGGACGAACTCAATACCCTCCTCAAGGAAAACGGCGTGGATCTTAGCGTCGTGGTCGAGAAAC
  AGAATGGCATGTACAAGGCCGCCCCTAAACGATTGGCGGCAACCACTGAGAAATTGGAGATGGGTTGGAA
  GGCCTGGGGGAAGTCTATCATCTTCGCACCTGAGCTTGCGAACAACACGTTCGTTATTGACGGCCCAGAG
  ACCGAGGAGTGTCCAACAGCAAATAGAGCCTGGAATTCTATGGAGGTAGAGGACTTCGGGTTCGGTCTGA
  CATCCACTAGAATGTTTCTGAGGATTCGGGAGACCAACACCACTGAATGTGACTCTAAGATTATCGGCAC
  AGCAGTCAAAAACAATATGGCAGTACACAGTGACCTGAGTTACTGGATCGAGAGCGGACTTAACGATACA
  TGGAAGCTTGAAAGAGCCGTTCTTGGCGAAGTTAAAAGCTGCACCTGGCCCGAGACCCACACACTCTGGG
  GCGATGGGGTTCTCGAGAGCGATCTGATAATCCCAATCACGCTGGCAGGCCCGAGAAGCAATCATAATAG
  ACGCCCAGGTTACAAAACTCAAAATCAAGGCCCTTGGGACGAGGGCCGAGTCGAAATTGACTTTGACTAT
  TGTCCTGGCACCACCGTGACAATCTCCGACAGTTGCGAGCACCGAGGACCCGCCGCCAGGACCACCACTG
  AGTCCGGTAAGCTGATTACTGATTGGTGTTGTCGCTCCTGTACTCTGCCGCCACTTCGATTCCAGACTGA
  AAACGGCTGCTGGTATGGCATGGAGATCAGGCCCACAAGACACGACGAAAAGACCCTTGTGCAGTCCAGA
  GTTAATGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102507 representing NP_776015
  Red=Cloning sites Green=Tags
  
  MDTGCAIDIGRQELRCGSGVFIHNDVEAWMDRYKFYPETPQGLAKIIQKAHAEGVCGLRSVSRLEHQMWE
  AIKDELNTLLKENGVDLSVVVEKQNGMYKAAPKRLAATTEKLEMGWKAWGKSIIFAPELANNTFVIDGPE
  TEECPTANRAWNSMEVEDFGFGLTSTRMFLRIRETNTTECDSKIIGTAVKNNMAVHSDLSYWIESGLNDT
  WKLERAVLGEVKSCTWPETHTLWGDGVLESDLIIPITLAGPRSNHNRRPGYKTQNQGPWDEGRVEIDFDY
  CPGTTVTISDSCEHRGPAARTTTESGKLITDWCCRSCTLPPLRFQTENGCWYGMEIRPTRHDEKTLVQSR
  VNA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001563
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001563.2, NP_776015
• RefSeq ORF: 1059
• MW: 39.7 kDa