Secondary Antibodies

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Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
AP31435AF-N

USD 470.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Azide Free

Applications This IgG fraction allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays are between 1/1000 and 1/5000.
Reactivities Monkey
Immunogen Highly purified IgG and pools of homogenous IgA and IgM isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
AP31435TC-N

USD 410.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, TRITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/10 and 1/40.
Reactivities Monkey
Conjugation TRITC
Immunogen Purified immunoglobulin fractions containing IgG, IgA and IgM isolated from pooled monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (Fc specific) Goat Polyclonal Antibody
AP31436AF-N

USD 470.00

2 Weeks

Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays are between 1/1000 and 1/5000.
Reactivities Monkey
Immunogen Highly purified immunoglobulin fractions containing IgG, IgA and IgM isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Fc specific) Goat Polyclonal Antibody
AP31437AF-N

USD 470.00

2 Weeks

Monkey IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2500.
Reactivities Monkey
Immunogen Purified IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Fc specific) Goat Polyclonal Antibody
AP31437TC-N

USD 410.00

2 Weeks

Monkey IgA (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/10 and 1/30.
Reactivities Monkey
Conjugation TRITC
Immunogen Purified polyclonal IgA isolated from pooled Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (Fc specific) Goat Polyclonal Antibody
AP31438TC-N

USD 410.00

2 Weeks

Monkey IgG (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/10 and 1/30.
Reactivities Monkey
Conjugation TRITC
Immunogen Purified polyclonal IgG isolated from pooled Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (H+L chain) Goat Polyclonal Antibody
AP31439AF-N

USD 240.00

2 Weeks

Monkey IgG (H+L chain) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Monkey
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgM (Fc specific) Goat Polyclonal Antibody
AP31440AF-N

USD 470.00

2 Weeks

Monkey IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
In Histochemistry are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Monkey
Immunogen Purified homogenous IgM isolated from monkey serum. Immunization with intact (19S) and split IgM (7S).
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgM (Fc specific) Goat Polyclonal Antibody
AP31440TC-N

USD 410.00

2 Weeks

Monkey IgM (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used for direct immunofluorescence staining of cytoplasmic IgM of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/10 and 1/40.
Reactivities Monkey
Conjugation TRITC
Immunogen Purified polyclonal IgM and pools of homogenous IgM isolated from pooled monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA (Secretory component) Goat Polyclonal Antibody
AP31450AF-N

USD 470.00

2 Weeks

Monkey IgA (Secretory component) goat polyclonal antibody, Azide Free

Applications Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes.
As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Working dilutions:
Histochemistry: 1/50 - 1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/200 - 1/1000.
Reactivities Chimpanzee, Monkey
Immunogen Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (H+L chain) Goat Polyclonal Antibody
AP31541AF-N

USD 240.00

2 Weeks

Canine IgG (H+L chain) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Canine
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543AF-N

USD 470.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Mouse
Immunogen Purified homogenous IgM isolated from mouse serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277BT-N

USD 290.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5,000-1/20,000.
Immunoblotting. 
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation Biotin
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277FC-N

USD 290.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, FITC

Applications FLISA: 1/100–1/400.
Flow Cytometry: ≤ 1 μg/106 cells.
Immunofluorescence: ≤ 1 μg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation FITC
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP32278BT-N

USD 220.00

3 Days

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000.
Immunoblotting.
Immunofluoresecence: ≤ 1 μg/106 cells.
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation Biotin
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.
Mouse IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP32278RP-N

USD 410.00

3 Days

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, PE

Applications FLISA: ? 0.1 µg/ml
Flow Cytometry: ? 0.1 µg/106 cells.
Reactivities Mouse
Conjugation PE
Immunogen Goats were hyperimmunized with Mouse IgM, IgG and IgA.
Porcine IgM (Fc specific) Goat Polyclonal Antibody
AP33049BT-N

USD 410.00

2 Weeks

Porcine IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used:
• In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
• To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
• In non-isotopic assay methodology (e.g. ELISA) to measure IgM in swine serum or other body fluids.
• As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Porcine
Conjugation Biotin
Immunogen Purified normal IgM isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Porcine IgM (Fc specific) Goat Polyclonal Antibody
AP33049HR-N

USD 550.00

2 Weeks

Porcine IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used:
• In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen using a reference antibody of swine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
• In non-isotopic assay methodology (e.g. ELISA) to measure IgM in swine serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Porcine
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (gamma chain specific) Goat Polyclonal Antibody
R1344HRP

USD 330.00

3 Days

Monkey IgG (gamma chain specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/50,000-1/150,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Monkey IgG gamma heavy chain.
Human IgM (Fc5 mu specific), F(ab)2 Fragment Goat Polyclonal Antibody
R1400P

USD 245.00

2 Weeks

Human IgM (Fc5 mu specific), F(ab)2 Fragment goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/20000-1/100000.
Western Blot: 1/2000-1/10000.
Immunohistochemistry: 1/1000-1/5000.
Reactivities Human
Immunogen Human IgM Fc5µ fragment
Rabbit IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
R1454P

USD 235.00

3 Days

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Aff - Purified

Applications Suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
ELISA: 1/15,000-1/30,000
Western blot: 1/500-1/2,000
Immunohistochemistry: 1/500-1/2,000.
Reactivities Rabbit
Immunogen Rabbit IgG whole molecule in goat.
IgG Goat Polyclonal Antibody
AB0147-200

USD 300.00

In Stock

Goat Polyclonal Anti-Human IgG Antibody

Applications WB 1:500-1:2,000
Reactivities Human
Immunogen Full human IgG affinity purified from serum of four normal donors
Human IgA (alpha chain) Goat Polyclonal Antibody
AP00640PU-N

USD 185.00

3 Days

Human IgA (alpha chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/80,000.
Wester Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Human
Immunogen
Human IgA alpha heavy chain.
Rat IgG2a Goat Polyclonal Antibody
AP05379HR-N

USD 310.00

5 Days

Rat IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP
Rat IgG2a Goat Polyclonal Antibody
AP05379PU-N

USD 250.00

5 Days

Rat IgG2a goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Mouse IgG2a Goat Polyclonal Antibody
AP05385HR-N

USD 310.00

5 Days

Mouse IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000.
Western Blotting.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG2a paraproteins
Mouse IgG2b Goat Polyclonal Antibody
AP05386HR-N

USD 310.00

5 Days

Mouse IgG2b goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000. 
Western Blot. 
Immunohistochemistry on Frozen Sections
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG2b paraproteins
Turkey IgG (H+L chain) Goat Polyclonal Antibody
AP08138BT-N

USD 160.00

3 Days

Turkey IgG (H+L chain) goat polyclonal antibody, Biotin

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Conjugation Biotin
Turkey IgG (H+L chain) Goat Polyclonal Antibody
AP08138FC-N

USD 130.00

3 Days

Turkey IgG (H+L chain) goat polyclonal antibody, FITC

Applications Immunofluorescence: < / = 1 µg/10e6 cells.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Conjugation FITC
Turkey IgG (H+L chain) Goat Polyclonal Antibody
AP08138PU-N

USD 120.00

3 Days

Turkey IgG (H+L chain) goat polyclonal antibody, Purified

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Turkey IgG (H+L chain) Goat Polyclonal Antibody
AP08138RP-N

USD 380.00

3 Days

Turkey IgG (H+L chain) goat polyclonal antibody, PE

Applications Immunofluorescence.
ELISA.
Western blotting.
Dot- and Slot-Immunoblotting.
Immunohistochemistry.
Conjugation PE
Mouse IgG (H+L chain) Goat Polyclonal Antibody
AP09454PU-N

USD 195.00

3 Days

Mouse IgG (H+L chain) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/150,000.
Western Blot: 1/3,000-1/15,000.
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Immunogen Mouse IgG, whole molecule.
Mouse IgG (Fc specific) Goat Polyclonal Antibody
AP20140PU-N

USD 205.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, Ig Fraction

Applications ELISA: 1/20000-1/100000. 
Western Blot: 1/2000-1/10000. 
Immunohistochemistry: 1/1000-1/5,000.
Reactivities Mouse
Immunogen Mouse IgG F(c) fragment
Human IgG (F(ab)2 specific) Goat Polyclonal Antibody
AP21427FC-N

USD 410.00

2 Weeks

Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/20-1/80. 
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation FITC
Immunogen Purified Fab prepared from normal IgG isolated from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Human IgG (F(ab)2 specific) Goat Polyclonal Antibody
AP21427TC-N

USD 410.00

2 Weeks

Human IgG (F(ab)2 specific) goat polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC
Immunogen Purified Fab prepared from normal IgG isolated from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482TC-N

USD 410.00

2 Weeks

Mouse IgE (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/20 - 1/60, depending on the method used.
Reactivities Mouse
Conjugation TRITC
Immunogen Pools of purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
AP31435FC-N

USD 410.00

2 Weeks

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, FITC

Applications Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Monkey IgG (H+L chain) Goat Polyclonal Antibody
AP31439FC-N

USD 410.00

2 Weeks

Monkey IgG (H+L chain) goat polyclonal antibody, FITC

Applications To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Monkey
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgA (Fc specific) Goat Polyclonal Antibody
AP31539FC-N

USD 410.00

2 Weeks

Canine IgA (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80, depending on the method used.
Reactivities Canine
Conjugation FITC
Immunogen Pools of purified IgA isolated from dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (Fc specific) Goat Polyclonal Antibody
AP31540FC-N

USD 410.00

2 Weeks

Canine IgG (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgG isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/15 and 1/60, depending on the method used.
Reactivities Canine
Conjugation FITC
Immunogen Pools of purified IgG isolated from dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (H+L chain) Goat Polyclonal Antibody
AP31541FC-N

USD 480.00

2 Weeks

Canine IgG (H+L chain) goat polyclonal antibody, FITC

Applications Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of dog origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/120.
Reactivities Canine
Conjugation FITC
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542FC-N

USD 410.00

2 Weeks

Canine IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
General Recommended Dilutions: are usually between 1/10 and 1/40, depending on the method used.
Reactivities Canine
Conjugation FITC
Immunogen Purified IgM isolated from Dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543BT-N

USD 410.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions:
For histochemical and Cytochemical use are usually between 1/100 and 1/250
In ELISA and comparable non-precipitating antibody-binding assays between 1/1500 and 1/6000.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified homogenous IgM isolated from Mouse serum.
Immunization with intact (19S) and split IgM (7S).
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543FC-N

USD 410.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, FITC

Applications In direct staining of cytoplasmic IgM in fixed mouse cells and tissue substrates; to identify circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of mouse in the middle layer of the test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/20 and 1/80.
Reactivities Mouse
Conjugation FITC
Immunogen Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split (7S) IgM. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543TC-N

USD 410.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, TRITC

Applications Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions are usually between 1/10 and 1/80.
Reactivities Mouse
Conjugation TRITC
Immunogen Purified pools of homogenous IgM isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
AP31795PU-N

USD 190.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/10,000 dilutions produced a detectable signal, as measured using HRP-labeled Chicken anti-Goat IgG Antibody Cat.-No AP31796HR-N (1/5000 dilution).
Western blot (1/5000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Immunoprecipitation (1/250).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, goat anti-chicken serum (75 μl) was placed in the lower trough and A30-106-10 (75 μl) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277HR-N

USD 230.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277PU-N

USD 200.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, Aff - Purified

Applications Immunoblotting.
Flow Cytometry. 
Immunohistochemistry.
Enzyme-Linked-Immunosorbent-Assay (ELISA).
Fluorescent-Linked-Immunosorbent-Assay (FLISA).
Reactivities Mouse
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277RP-N

USD 410.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, PE

Applications FLISA.
Flow Cytometry. 
Immunofluorescence: ≤ 0.1 µg/106 cells.
Immunohistochemistry.
Reactivities Mouse
Conjugation PE
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277TC-N

USD 290.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, TRITC

Applications FLISA: 1/100-1/400.
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation TRITC
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.