Secondary Antibodies

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Biotin Conjugated Rabbit Anti-Goat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Goat IgG
Conjugation Biotin
Immunogen Goat IgG, whole molecule

Monkey IgG (Fab specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining to identify and measure free or Ig bound subunits of both kappa and lambda type at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. In general this kind of products is not recommended as direct or indirect screening reagents for immunoglobulin isotypes on the surface of membranes of vital lymphoid cells. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified Fab from normal IgG isolated from pooled Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Biotin Conjugated Rabbit Anti-Rat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rat IgG
Conjugation Biotin
Immunogen Rat IgG, whole molecule

Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified homogenous IgE isolated from mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Bovine IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications Immunocytochemical and Immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of bovine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgM in Bovine serum or other body fluids.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/100-1/500.
Reactivities Bovine
Conjugation Biotin
Immunogen Purified normal IgM isolated from pooled Bovine serum.
Feund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, Biotin

Applications Can be usde in immunocytochemical and immunohistochemical staining of immunoglobulins at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of monkey origin in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure immunoglobulins in monkey serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000 - 1/20000.
Reactivities Monkey
Conjugation Biotin
Immunogen Highly purified IgG, IgA and IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
Histochemical and cytochemical: 1/100 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000 - 1/10000.
Reactivities Monkey
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and cytochemical Use: 1/100- 1/2500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000- 1/5000.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/500,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation Biotin
Immunogen Chicken IgG whole molecule.

Bovine IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting (1/2,000-1/10,000).
ELISA (1/20,000-1/100,000).
Immunohistochemistry (1/1,000-1/5,000).
Immunomicroscopy.
as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. 
This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/20,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation Biotin
Immunogen Bovine IgG whole molecule

Biotin Conjugated Rabbit Anti-Goat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Goat IgG
Conjugation Biotin
Immunogen Goat IgG, whole molecule

Biotin Conjugated Rabbit Anti-Rat IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Rat IgG
Conjugation Biotin
Immunogen Rat IgG, whole molecule

Biotin Conjugated Rabbit Anti-human IgG

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Human IgG
Conjugation Biotin
Immunogen Human IgG, whole molecule

Duck IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications ELISA.
Dot blot. 
Immunoblotting.

Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 
Histochemical and Cytochemical Use: 1/10-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000.
Reactivities Duck
Conjugation Biotin
Immunogen Purified normal Ig fractions isolated from pooled Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Sheep IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.  
Immunohistochemistry on Paraffin Sections.
Can be used In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000.
Reactivities Sheep
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000.
Reactivities Sheep
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Sheep IgM (Fc specific) rabbit polyclonal antibody, Biotin

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/50-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/4,000.
Reactivities Sheep
Conjugation Biotin
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Sheep IgM (H+L chain) rabbit polyclonal antibody, Biotin

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8,000.
Reactivities Sheep
Conjugation Biotin
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Porcine IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000.
Reactivities Porcine
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Porcine IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.

Can be used in immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of swine origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Porcine
Conjugation Biotin
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Bovine IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/15,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Bovine
Conjugation Biotin
Immunogen Bovine IgG F(c) fragment.

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Immunoblotting.
ELISA.
Immunohistochemistry.
Immunofluorescence.
Reactivities Bovine
Conjugation Biotin
Immunogen Bovine IgG F(ab’)2 fragment

Mouse IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation Biotin
Immunogen Mouse IgG whole molecule.

Goat IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other assays using streptavidin/avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/1,00,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation Biotin
Immunogen Goat IgG whole molecule.

Mouse IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, Biotin

Applications ELISA: 1/1000 - 1/2000.
Flow Cytometry: 1/50 - 1/100; Use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Immunohistochemistry on frozen and paraffin embedded sections: 1/50 - 1/200.
Reactivities Mouse
Conjugation Biotin
Immunogen Purified mouse IgG

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Mouse IgG whole molecule.

Donkey IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:45,000 to 1:150,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Donkey IgG whole molecules.

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Chicken IgG F(c) fragment.

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:8,000 to 1:34,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Chicken IgG F(ab')2 fragment.

Feline IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoelectron microscopy and Immunohistochemistry.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS as a substrate. A working dilution of 1:27,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Feline IgG whole molecule.

Feline IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:57,000 to 1:220,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Cat IgG F(c) fragment.

Feline IgG F(ab')2 specific rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Diluttions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:40,000 to 1:160,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Cat IgG F(ab')2 fragment.

Canine IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:17,500 to 1:75,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Dog IgG whole molecule.

Canine IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:40,000 to 1:160,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Dog IgG F(c) fragment.

Canine IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:15,000 to 1:65,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Dog IgG F(ab')2 fragment.

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/28,000-1/116,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Goat IgG whole molecule.

Goat IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation Biotin
Immunogen Goat IgG F(c) fragment.

Goat IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:40,000 to 1:200,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Goat IgG F(ab')2 fragment.

Goat IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Goat IgG F(ab')2 fragment.

Guinea pig IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Guinea Pig IgG whole molecule.

Guinea pig IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard Capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Conjugation Biotin
Immunogen Guinea Pig IgG F(c) fragment.

Guinea pig IgG F(ab')2 specific rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:34,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Guinea Pig IgG F(ab')2 fragment.

Hamster IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0µg of hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/15,000 to 1/60,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Hamster IgG whole molecule

Golden Syrian Hamster IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:400,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Hamster IgG F(ab’)2 fragment

Equine IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:14,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Horse IgG whole molecule.

Equine IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Horse IgG F(c) fragment.

Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:45,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Horse IgG F(ab')2 fragment.

Human IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:50,000 to 1:220,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgG, whole molecule

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:17,000 to 1:75,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgG, whole molecule.

Human IgG (gamma chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:5,000 to 1:35,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgG γ chain.