Secondary Antibodies

View as table Download

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Human IgG, whole molecule.

Human IgG (gamma chain specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,500 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Human IgG gamma chains.

Human IgG (gamma chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:5,000 to 1:35,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgG γ chain.

Human IgG (gamma chain specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:60,000 to 1:300,000 is suggested for this product.
Conjugation HRP
Immunogen Human IgG gamma chain.

Human IgG (gamma chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. 
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent Western blotting. Also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions: 
FLISA: 1/10,000-1/50,000. 
IF Microscopy: 1/1,000-1/5,000. 
Flow Cytometry: 1/500-1/2,500.
Conjugation TRITC
Immunogen Human IgG heavy chain

Human IgG (gamma chain specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation Texas Red
Immunogen Human IgG gamma heavy chain.

Human IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:16,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Human IgG F(c) fragment.

Human IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/10,000-1/100,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Conjugation Biotin
Immunogen Human IgG F(c) fragment.

Human IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500
Note:This antibody has been assayed against 1.0 ug of Human IgG F(c) in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Human IgG F(c) fragment

Human IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG F(c) fragment.

Human IgG (Fc specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Human IgG F(c) fragment.

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 is suggested for this product.
Conjugation AP
Immunogen Human IgG F(ab')2 fragment.

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1/30,000 to 1/120,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgG F(ab')2 fragment.

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Human IgG F(ab')2 fragment

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1:500-1:2,500) and Flow cytometry (1:2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG F(ab')2 fragment.

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended dilutions:
Flow cytometry: 1:2,000 - 1:10,000.
Immunofluoresecene microscopy: 1:500 - 1:2,500.
Conjugation Texas Red
Immunogen Human IgG F(ab')2 fragment

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,500 to 1:11,000 of the stated concentration is suggested for this product.
Conjugation AP
Immunogen Human IgG F(ab')2 fragment.

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin (cat.-no. RA021HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:12,000 to 1:55,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgG F(ab')2 fragment

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,500 to 1:72,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Human IgG F(ab')2 fragment.

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgG F(ab')2 fragment.

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Human IgG F(ab')2 fragment.

Human IgM (Fc5 mu specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Human IgM (Fc5µ) fragment

Human IgM (Fc5 mu specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgM (Fc5µ) fragment

Human IgM (Fc5 mu specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Human IgM (Fc5µ) fragment

Human IgM (Fc5 mu specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/15,000-1/60,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Conjugation HRP
Immunogen Human IgM (Fc5µ) fragment

Human IgM (Fc5 mu specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/40,000-1/160,000.
Western Blot: 1/500-1/3,000.
Immunohistochemistry: 1/500-1/3,000.
Reactivities Human
Immunogen Human IgM (Fc5m) fragment

Human IgM (Fc5 mu specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Human IgM (Fc5µ) fragment

Human IgM (Fc5 mu specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Human IgM (Fc5µ) fragment

Human IgA rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgA in a standard capture ELISA using p-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Human IgA heavy chain.

Human IgA (alpha chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Human IgA alpha heavy chain

Human IgA (alpha chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Flow cytometry: 1:2,000 - 1:10,000.
Immunflourescence microscopy: 1:500 - 1:2,500.
Conjugation FITC
Immunogen Human IgA alpha chain

Human IgA (alpha chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/56,000
Western Blot: 1/1,000-1/5,000
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Human IgA α alpha chain

Human IgA (alpha chain) rabbit polyclonal antibody, TRITC

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation TRITC
Immunogen Human IgA alpha chain.

Human IgA (alpha chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Human IgA heavy chain.

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (1/100-1/1,000; Western or dot blot), ELISA (1/1,000-1/4000) and Immunohistochemistry (1/100-1/500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Conjugation AP
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Conjugation FITC
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/500-1/2,500, Western or dot blot), ELISA (1/5,000-1/20,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/200-1/1,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Conjugation HRP
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Mouse IgG whole molecule.

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using p-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Mouse IgG γ (gamma heavy chain).

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Mouse IgG γ heavy chain.

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Mouse IgG γ gamma chain

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Mouse IgG gamma chain.

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Mouse IgG γ chain.

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Mouse IgG heavy chain.

Mouse IgG (gamma chain specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Mouse IgG gamma heavy chain.

Mouse IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Mouse IgG F(c) fragment.

Mouse IgG (Fc specific) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:14,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Mouse IgG F(c) fragment.

Mouse IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Mouse IgG F(c) Fragment.

Mouse IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/1,000-1/10,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/10,000-1/50,000.
Western Blot : 1/1,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/50,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Mouse IgG F(c) fragment.