Secondary Antibodies

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FITC Conjugated Rabbit Anti-Goat IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Goat IgG
Conjugation FITC
Immunogen Goat IgG, whole molecule

FITC Conjugated Rabbit Anti-human IgG

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Human IgG
Conjugation FITC
Immunogen Human IgG, whole molecule

Mouse IgG2a rabbit polyclonal antibody, Purified

Applications ELISA.
Reactivities Mouse
Immunogen Mouse IgG2a paraproteins

Human IgD (F(ab)2 Fragment) rabbit polyclonal antibody, Purified

Applications ELISA
Reactivities Human
Immunogen Human IgD

Goat IgA rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Goat
Conjugation HRP
Immunogen Purified goat IgA.

Goat IgM rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000-1/100,000.
Reactivities Goat
Conjugation HRP
Immunogen Purified Goat IgM

Human IgD rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
Use Polymer anti Mouse/Rabbit immunoglobulin as a detection system.
Positive Control: Lymph node.
Reactivities Human
Immunogen Purified IgD

Human IgA rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Paraffin Sections: 5 µg/ml.
Reactivities Human
Immunogen IgA isolated from a pool of normal Human sera.

Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis, single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate immunoglobulins in normal monkey serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommmended dilutions:
Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum.
Double radial immunodiffusion: use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Purified polyclonal IgG, IgA and IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (H+L chain) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal monkey serum; to prepare an immunoadsorbent for the production of Ig-free monkey serum, plasma or non-Ig preparations.
Recommended dilutions:
In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum.
In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Purified normal IgG isolated from pooled rhesus monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Delta chain specific) rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on paraffin embedded sections:
diluted of 1:40-1:100 in an ABC method (30-60 minutes at room temperature)
Recommended Positive Control: Tonsil
Reactivities Human
Immunogen IgD isolated from a pool of normal human plasma.

Human IgD (Delta chain specific) rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on paraffin embedded sections:
diluted of 1:40-1:100 in an ABC method (30-60 minutes at room temperature)
Recommended Positive Control: Tonsil
Reactivities Human
Immunogen IgD isolated from a pool of normal human plasma.

Donkey IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Donkey IgG whole molecule.

Donkey IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:45,000 to 1:150,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Donkey IgG whole molecules.

Donkey IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Donkey IgG whole molecule.

Donkey IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Note: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Donkey IgG whole molecule.

Donkey IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Donkey IgG whole molecules.

Donkey IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Donkey IgG whole molecule.

Donkey IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Donkey IgG whole molecule.

Mouse IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring high titer binding and lot-to-lot consistency.
Recommended Dilutions:
IF Microscopy : 1:500 - 1:2,500
Other Dilution: User Optimized
Conjugation Texas Red
Immunogen Mouse IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product, in Western Blot 1:1,000, Immunohistochemistry 1:500.
Conjugation AP
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Chicken IgG whole molecule

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:9,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Chicken IgG F(c) fragment.

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3- ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Chicken IgG F(c) fragment.

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Chicken IgG F(c) fragment.

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:37,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Chicken IgG F(c) fragment.

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Chicken IgG F(c) fragment.

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Chicken IgG F(c) fragment.

Chicken IgG / Chicken IgY (Fc specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Chicken IgG F(c) fragment.

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:1,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Chicken IgG F(ab')2 fragment.

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:8,000 to 1:34,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Chicken IgG F(ab')2 fragment.

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Chicken IgG F(ab)2 fragment

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Chicken IgG F(ab')2 fragment

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Chicken IgG F(ab')2 fragment.

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Chicken IgG F(ab')2 fragment.

Chicken IgG / Chicken IgY (F(ab)2 specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Chicken IgG F(ab')2 fragment.

Bovine IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation FITC
Immunogen Bovine IgG, whole molecule

Bovine IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation TRITC
Immunogen Bovine IgG whole molecule.

Bovine IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation Texas Red
Immunogen Bovine IgG whole molecule.

Bovine IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation FITC
Immunogen Bovine IgG F(c) fragment.

Bovine IgG (Fc specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation TRITC
Immunogen Bovine IgG F(c) fragment.

Bovine IgG (Fc specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation Texas Red
Immunogen Bovine IgG F(c) fragment.

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation FITC
Immunogen Bovine IgG F(ab')2 fragment.

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation TRITC
Immunogen Bovine IgG F(ab')2 fragment.

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation Texas Red
Immunogen Bovine IgG F(ab')2 fragment.

Feline IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:18,000 is suggested for this product.
Conjugation AP
Immunogen Cat IgG whole molecule.

Feline IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoelectron microscopy and Immunohistochemistry.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS as a substrate. A working dilution of 1:27,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Feline IgG whole molecule.

Feline IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Cat IgG whole molecule.

Feline IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Cat IgG whole molecule.