Secondary Antibodies

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Human IgG4 (pFC) Mouse Monoclonal Antibody [Clone ID: HP6023 (also known as HO6023)]
AM01158AP-N

USD 300.00

5 Days

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), AP, Purified

Applications ELISA: 1/1000-1/2000 (See also Ref.2-9). 
FLISA. 
Applications Reported in Literature: 
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation AP
IGHG4 Mouse Monoclonal Antibody [Clone ID: HP6025]
SM2069AP

USD 330.00

5 Days

IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections:  reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry: reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP
Rabbit IgG (H+L chain) Goat Polyclonal Antibody
R1364AP

USD 270.00

3 Days

Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/3,000-1/25,000.
Western blot: 1/1,000-1/4,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Rabbit
Conjugation AP
Immunogen Rabbit IgG, whole molecule.
Mouse IgG1 Goat Polyclonal Antibody
AP05384AP-N

USD 365.00

5 Days

Mouse IgG1 goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG1 paraproteins.
Mouse IgG (Fc specific) Goat Polyclonal Antibody
R1612AP

USD 300.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, AP

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/2,000-1/100,000
Western blot: 1/500-1/2,500
Immunohistochemistry: 1/200-1/1,000.
Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code #NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/5,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG F(c) fragment
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256AP

USD 270.00

3 Days

Mouse IgG (H+L chain) sheep polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 �g of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Mouse IgG whole molecule.
Goat IgG (H+L chain) Rabbit Polyclonal Antibody
R1317AP

USD 250.00

3 Days

Goat IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/13,000.
Western blot: 1/500-1/2,500. 
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Goat IgG whole molecule.
Bovine IgG1 Sheep Polyclonal Antibody
SP1049APX

USD 365.00

In Stock

Bovine IgG1 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG1.
Mouse IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP32278AP-N

USD 250.00

3 Days

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.
Bovine IgG (H+L chain) Rabbit Polyclonal Antibody
R1304AP

USD 285.00

3 Days

Bovine IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product.
Reactivities Bovine
Conjugation AP
Immunogen Bovine IgG whole molecule.
Bovine IgG (Fc specific) Rabbit Polyclonal Antibody
R1305AP

USD 315.00

3 Days

Bovine IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation AP
Immunogen Bovine IgG F(c) fragment.
Bovine IgG (F(ab)2 specific) Rabbit Polyclonal Antibody
R1306AP

USD 310.00

3 Days

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:11,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation AP
Immunogen Bovine IgG F(ab')2 fragment.
Human IgA1 (alpha chain) Mouse Monoclonal Antibody [Clone ID: B3506B4]
AM01152AP-N

USD 630.00

5 Days

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, AP

Applications Immunohistochemistry on frozen sections.
ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation AP
Mouse IgG2b Goat Polyclonal Antibody
AP05386AP-N

USD 365.00

5 Days

Mouse IgG2b goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.  
Western Blot. 
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG2b paraproteins.
Sheep/Goat IgG Donkey Polyclonal Antibody
AP05395AP-N

USD 330.00

5 Days

Sheep/Goat IgG donkey polyclonal antibody, AP

Applications Western blot.
ELISA: 1/2000-1/5000.
Immunohistochemistry on Frozen Sections.
Reactivities Goat, Sheep
Conjugation AP
Immunogen Purified IgG from a Sheep serum pool
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277AP-N

USD 240.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, AP

Applications

ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Mouse IgG Goat Polyclonal Antibody
AP33451AP-N

USD 165.00

2 Weeks

Mouse IgG goat polyclonal antibody, AP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Immunogen Purified Mouse IgG fraction
Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) Goat Polyclonal Antibody
AP33463AP-N

USD 355.00

3 Days

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, AP, Aff - Purified

Applications Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/10,000-1/40,000.
Western Blot: 1/200-1/2,000. 
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP
Immunogen Native Mouse IgG1 heavy chain Protein.
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253AP

USD 270.00

3 Days

Mouse IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for use in Immunoblotting (Western or dot blot), ELISA, Immunohistochemistry and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Mouse IgG whole molecule
Human IgM (Mu chain specific) Goat Polyclonal Antibody
R1340AP

USD 300.00

3 Days

Human IgM (Mu chain specific) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other AP-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/1,000-1/3,000.
Western blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Human IgM μ mu chain
Human IgG3 (Hinge Region) Mouse Monoclonal Antibody [Clone ID: HP6050]
SM1026AP

USD 280.00

5 Days

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP
Bovine IgG2 Sheep Polyclonal Antibody
SP2000APX

USD 365.00

5 Days

Bovine IgG2 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG2.
Porcine IgA Goat Polyclonal Antibody
SP2016APX

USD 365.00

5 Days

Porcine IgA goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/1000-1/10000. 
Immunohistochemistry on Frozen Sections: 1/100-1/1000.
Reactivities Porcine
Conjugation AP
Immunogen Purified Porcine IgA.
Human IgM (Mu chain specific) Mouse Monoclonal Antibody [Clone ID: SA-DA4]
AM08145AP-N

USD 330.00

3 Days

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP
Human IgG1 (gamma 1 chain specific) Mouse Monoclonal Antibody [Clone ID: 4E3]
AM08151AP-N

USD 280.00

3 Days

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, AP

Applications ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation AP
Mouse IgG (adsorbed) Goat Polyclonal Antibody
AP05383AP-N

USD 395.00

5 Days

Mouse IgG (adsorbed) goat polyclonal antibody, AP

Applications ELISA: 1/200-1/2500.
Western Blot: 1/100-1/500.
Reactivities Mouse
Conjugation AP
Immunogen Whole Mouse IgG
Human IgE Goat Polyclonal Antibody
AP05393AP-N

USD 365.00

5 Days

Human IgE goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/5000.
Reactivities Human, Monkey
Conjugation AP
Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
AP31795AP-N

USD 230.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate.
Western Blot (1/1000).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA.
Reactivities Chicken
Conjugation AP
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1406AP

USD 325.00

3 Days

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested for this product. 
Recommended Dilutions: 
ELISA: 1/2000-1/10000. 
Western Blot: 1/500-1/2500. 
Immunochemistry: 1/200-1/1000.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG F(c) fragment.
Bovine IgG Sheep Polyclonal Antibody
SP1047APX

USD 365.00

5 Days

Bovine IgG sheep polyclonal antibody, AP

Applications

ELISA: 1/5000-1/50000.
Western Blot: 1/5000-1/50000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG.
Bovine IgM Sheep Polyclonal Antibody
SP1048APX

USD 365.00

5 Days

Bovine IgM sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/5000-1/50000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgM.
Porcine IgG F(c) specific Goat Polyclonal Antibody
SP2017AP

USD 365.00

5 Days

Porcine IgG F(c) specific goat polyclonal antibody, AP

Applications ELISA: 1/5000-1/50000.
Reactivities Porcine
Conjugation AP
Immunogen Purified Porcine IgG
Mouse IgE (Epsilon heavy chain) Rat Monoclonal Antibody [Clone ID: 23G3]
AM08013AP-N

USD 360.00

3 Days

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, AP

Applications ELISA: 1/500-1/1000.
Reactivities Mouse
Conjugation AP
Mouse IgM (Mu chain specific) Rat Monoclonal Antibody [Clone ID: SB73a]
AM08014AP-N

USD 330.00

3 Days

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone SB73a, AP

Applications ELISA: 1/1000-1/2000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation AP
Mouse IgM (Mu chain specific) Rat Monoclonal Antibody [Clone ID: 1B4B1]
AM08015AP-N

USD 340.00

3 Days

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, AP

Applications ELISA: 1/1000-1/2000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation AP
Mouse IgG2a (gamma 2a chain specific) Rat Monoclonal Antibody [Clone ID: SB84a]
AM08017AP-N

USD 310.00

3 Days

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, AP

Applications ELISA: 1/500-1/1000.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation AP
Mouse IgA (alpha chain specific) Rat Monoclonal Antibody [Clone ID: 11-44-2]
AM08018AP-N

USD 360.00

3 Days

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, AP

Applications ELISA: 1/500-1/1000.
Reactivities Mouse
Conjugation AP
Rabbit IgG Mouse Monoclonal Antibody [Clone ID: 2A9]
AM08102AP-N

USD 310.00

3 Days

Rabbit IgG mouse monoclonal antibody, clone 2A9, AP

Applications ELISA: 1/2,000-1/4,000.
Reactivities Rabbit
Conjugation AP
Human IgM (Mu chain specific) Mouse Monoclonal Antibody [Clone ID: UHB]
AM08146AP-N

USD 330.00

3 Days

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP
Human IgD (Delta chain specific) Mouse Monoclonal Antibody [Clone ID: IADB6]
AM08148AP-N

USD 300.00

3 Days

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP
Human IgG (Fc specific) Mouse Monoclonal Antibody [Clone ID: JDC-10]
AM08149AP-N

USD 280.00

3 Days

Human IgG (Fc specific) mouse monoclonal antibody, clone JDC-10, AP

Applications ELISA: 1/1000-1/4,000.
Reactivities Human
Conjugation AP
Human IgG (Fc specific) Mouse Monoclonal Antibody [Clone ID: H2]
AM08150AP-N

USD 280.00

3 Days

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, AP

Applications ELISA: 1/2000-1/8,000.
Reactivities Human
Conjugation AP
Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: 31-7-4]
AM08152AP-N

USD 330.00

3 Days

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP
Mouse IgE Goat Polyclonal Antibody
AP05376AP-N

USD 570.00

5 Days

Mouse IgE goat polyclonal antibody, AP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation AP