Secondary Antibodies

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Human IgG4 (pFC) Mouse Monoclonal Antibody [Clone ID: HP6023 (also known as HO6023)]
AM01158AP-N

USD 300.00

5 Days

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), AP, Purified

Applications ELISA: 1/1000-1/2000 (See also Ref.2-9). 
FLISA. 
Applications Reported in Literature: 
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation AP
IGHG4 Mouse Monoclonal Antibody [Clone ID: HP6025]
SM2069AP

USD 330.00

5 Days

IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections:  reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry: reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP
Rabbit IgG (H+L chain) Goat Polyclonal Antibody
R1364AP

USD 270.00

3 Days

Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/3,000-1/25,000.
Western blot: 1/1,000-1/4,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Rabbit
Conjugation AP
Mouse IgG1 Goat Polyclonal Antibody
AP05384AP-N

USD 365.00

5 Days

Mouse IgG1 goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP
Mouse IgG (Fc specific) Goat Polyclonal Antibody
R1612AP

USD 300.00

3 Days

Mouse IgG (Fc specific) goat polyclonal antibody, AP

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/2,000-1/100,000
Western blot: 1/500-1/2,500
Immunohistochemistry: 1/200-1/1,000.
Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code #NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/5,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256AP

USD 270.00

3 Days

Mouse IgG (H+L chain) sheep polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 �g of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Goat IgG (H+L chain) Rabbit Polyclonal Antibody
R1317AP

USD 250.00

3 Days

Goat IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/13,000.
Western blot: 1/500-1/2,500. 
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Bovine IgG1 Sheep Polyclonal Antibody
SP1049APX

USD 365.00

In Stock

Bovine IgG1 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP
Mouse IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP32278AP-N

USD 250.00

3 Days

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Bovine IgG (H+L chain) Rabbit Polyclonal Antibody
R1304AP

USD 285.00

3 Days

Bovine IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product.
Reactivities Bovine
Conjugation AP
Bovine IgG (Fc specific) Rabbit Polyclonal Antibody
R1305AP

USD 315.00

3 Days

Bovine IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation AP
Bovine IgG (F(ab)2 specific) Rabbit Polyclonal Antibody
R1306AP

USD 310.00

3 Days

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:11,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation AP
Human IgA1 (alpha chain) Mouse Monoclonal Antibody [Clone ID: B3506B4]
AM01152AP-N

USD 630.00

5 Days

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, AP

Applications Immunohistochemistry on frozen sections.
ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation AP
Mouse IgG2b Goat Polyclonal Antibody
AP05386AP-N

USD 365.00

5 Days

Mouse IgG2b goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.  
Western Blot. 
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP
Sheep/Goat IgG Donkey Polyclonal Antibody
AP05395AP-N

USD 330.00

5 Days

Sheep/Goat IgG donkey polyclonal antibody, AP

Applications Western blot.
ELISA: 1/2000-1/5000.
Immunohistochemistry on Frozen Sections.
Reactivities Goat, Sheep
Conjugation AP
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277AP-N

USD 240.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, AP

Applications

ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Mouse IgG Goat Polyclonal Antibody
AP33451AP-N

USD 165.00

2 Weeks

Mouse IgG goat polyclonal antibody, AP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) Goat Polyclonal Antibody
AP33463AP-N

USD 355.00

3 Days

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, AP, Aff - Purified

Applications Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/10,000-1/40,000.
Western Blot: 1/200-1/2,000. 
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253AP

USD 270.00

3 Days

Mouse IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for use in Immunoblotting (Western or dot blot), ELISA, Immunohistochemistry and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Human IgM (Mu chain specific) Goat Polyclonal Antibody
R1340AP

USD 300.00

3 Days

Human IgM (Mu chain specific) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other AP-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/1,000-1/3,000.
Western blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Human IgG3 (Hinge Region) Mouse Monoclonal Antibody [Clone ID: HP6050]
SM1026AP

USD 280.00

5 Days

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP
Bovine IgG2 Sheep Polyclonal Antibody
SP2000APX

USD 365.00

5 Days

Bovine IgG2 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP
Porcine IgA Goat Polyclonal Antibody
SP2016APX

USD 365.00

5 Days

Porcine IgA goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/1000-1/10000. 
Immunohistochemistry on Frozen Sections: 1/100-1/1000.
Reactivities Porcine
Conjugation AP
Human IgM (Mu chain specific) Mouse Monoclonal Antibody [Clone ID: SA-DA4]
AM08145AP-N

USD 330.00

3 Days

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP
Human IgG1 (gamma 1 chain specific) Mouse Monoclonal Antibody [Clone ID: 4E3]
AM08151AP-N

USD 280.00

3 Days

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, AP

Applications ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation AP
Mouse IgG (adsorbed) Goat Polyclonal Antibody
AP05383AP-N

USD 395.00

5 Days

Mouse IgG (adsorbed) goat polyclonal antibody, AP

Applications ELISA: 1/200-1/2500.
Western Blot: 1/100-1/500.
Reactivities Mouse
Conjugation AP
Human IgE Goat Polyclonal Antibody
AP05393AP-N

USD 365.00

5 Days

Human IgE goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/5000.
Reactivities Human, Monkey
Conjugation AP
Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
AP31795AP-N

USD 230.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate.
Western Blot (1/1000).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA.
Reactivities Chicken
Conjugation AP
Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
R1406AP

USD 325.00

3 Days

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested for this product. 
Recommended Dilutions: 
ELISA: 1/2000-1/10000. 
Western Blot: 1/500-1/2500. 
Immunochemistry: 1/200-1/1000.
Reactivities Mouse
Conjugation AP
Bovine IgG Sheep Polyclonal Antibody
SP1047APX

USD 365.00

5 Days

Bovine IgG sheep polyclonal antibody, AP

Applications

ELISA: 1/5000-1/50000.
Western Blot: 1/5000-1/50000.
Reactivities Bovine
Conjugation AP
Bovine IgM Sheep Polyclonal Antibody
SP1048APX

USD 365.00

5 Days

Bovine IgM sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/5000-1/50000.
Reactivities Bovine
Conjugation AP
Porcine IgG F(c) specific Goat Polyclonal Antibody
SP2017AP

USD 365.00

5 Days

Porcine IgG F(c) specific goat polyclonal antibody, AP

Applications ELISA: 1/5000-1/50000.
Reactivities Porcine
Conjugation AP
Mouse IgE (Epsilon heavy chain) Rat Monoclonal Antibody [Clone ID: 23G3]
AM08013AP-N

USD 360.00

3 Days

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, AP

Applications ELISA: 1/500-1/1000.
Reactivities Mouse
Conjugation AP
Mouse IgM (Mu chain specific) Rat Monoclonal Antibody [Clone ID: SB73a]
AM08014AP-N

USD 330.00

3 Days

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone SB73a, AP

Applications ELISA: 1/1000-1/2000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation AP
Mouse IgM (Mu chain specific) Rat Monoclonal Antibody [Clone ID: 1B4B1]
AM08015AP-N

USD 340.00

3 Days

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, AP

Applications ELISA: 1/1000-1/2000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation AP
Mouse IgG2a (gamma 2a chain specific) Rat Monoclonal Antibody [Clone ID: SB84a]
AM08017AP-N

USD 310.00

3 Days

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, AP

Applications ELISA: 1/500-1/1000.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation AP
Mouse IgA (alpha chain specific) Rat Monoclonal Antibody [Clone ID: 11-44-2]
AM08018AP-N

USD 360.00

3 Days

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, AP

Applications ELISA: 1/500-1/1000.
Reactivities Mouse
Conjugation AP
Rabbit IgG Mouse Monoclonal Antibody [Clone ID: 2A9]
AM08102AP-N

USD 310.00

3 Days

Rabbit IgG mouse monoclonal antibody, clone 2A9, AP

Applications ELISA: 1/2,000-1/4,000.
Reactivities Rabbit
Conjugation AP
Human IgM (Mu chain specific) Mouse Monoclonal Antibody [Clone ID: UHB]
AM08146AP-N

USD 330.00

3 Days

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP
Human IgD (Delta chain specific) Mouse Monoclonal Antibody [Clone ID: IADB6]
AM08148AP-N

USD 300.00

3 Days

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP
Human IgG (Fc specific) Mouse Monoclonal Antibody [Clone ID: JDC-10]
AM08149AP-N

USD 280.00

3 Days

Human IgG (Fc specific) mouse monoclonal antibody, clone JDC-10, AP

Applications ELISA: 1/1000-1/4,000.
Reactivities Human
Conjugation AP
Human IgG (Fc specific) Mouse Monoclonal Antibody [Clone ID: H2]
AM08150AP-N

USD 280.00

3 Days

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, AP

Applications ELISA: 1/2000-1/8,000.
Reactivities Human
Conjugation AP
Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: 31-7-4]
AM08152AP-N

USD 330.00

3 Days

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP
Mouse IgE Goat Polyclonal Antibody
AP05376AP-N

USD 570.00

5 Days

Mouse IgE goat polyclonal antibody, AP

Applications ELISA: 1:100 - 1:1000.
Reactivities Mouse
Conjugation AP