Secondary Antibodies

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Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation FITC

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
 ≤ 1 µg/106 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation FITC

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, FITC

Applications Flow Cytometry: < / = 1.0 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Reactivities Rat
Conjugation FITC

Chicken IgG / Chicken IgY (gamma chain specific) mouse monoclonal antibody, clone G-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation FITC

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation FITC

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, FITC

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, FITC

Applications FLISA: 1/200-1/600.
Indirect Immunofluorescent staining
of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
Reactivities Human
Conjugation FITC

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, FITC

Applications FLISA:1:200-1:400
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgM mouse monoclonal antibody, clone ICL-931, FITC

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/40. 
Staining of Human cells and tissue sections.
Reactivities Human
Conjugation FITC

Human IgG mouse monoclonal antibody, clone EFE-565, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Human IgA mouse monoclonal antibody, clone BFG-267, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Mouse IgG2c goat polyclonal antibody, FITC

Applications Flow Cytometry.
Reactivities Mouse
Conjugation FITC

Goat IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry (1/1000).
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Goat
Conjugation FITC
Immunogen Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated
with Fluorescein isothiocyanate.

Human IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Human
Conjugation FITC
Immunogen Purified, non-denatured Human IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate.

Mouse IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Mouse
Conjugation FITC
Immunogen Purified, non-denatured Mouse IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Fluorescein Isothiocyanate.

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, FITC, Purified

Applications ELISA: 1/1000-1/10000. 
Immunofluorescence.
Reactivities Human
Conjugation FITC

Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Goat
Conjugation FITC
Immunogen Goat IgG whole molecule.

Goat IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Conjugation FITC
Immunogen Goat IgG whole molecule

Hamster IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
Flow Cytometry: 1/2,000-1/10,000.
IF Microscopy: 1/500-1/2,500.
Conjugation FITC
Immunogen Hamster IgG whole molecule.

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for immunomicroscopy,  Fluorescent based plate assays (FLISA) and  Fluorescent Western Blotting.
Also Suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. 
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
IF Microscopy : 1/1,000-1/5,000.
Conjugation FITC
Immunogen Human IgG F(ab')2 fragment

Monkey IgM (Mu chain specific) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Monkey IgM µ mu chain.

Monkey IgA (alpha chain specific) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
FLISA: 1:10,000-1:50,000
IF: 1:1,000-1:5,000
Conjugation FITC
Immunogen Monkey IgA α chain.

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation FITC
Immunogen Mouse IgG2a heavy chain.

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.  
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
IF Microscopy: 1/1,000-1/5,000. 
Flow Cytometry: 1/500-1/2,500. 

 
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG F(c) fragment.

Rabbit IgG (H+L chain) F(ab)2 Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA:  1/1000 - 1/50,000
IF Microscopy:  1/1000 - 1/5000
Conjugation FITC
Immunogen Rabbit IgG whole molecule.

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA:  1:10,000/1:50,000
IF Microscopy:  1:1000/1:5000
Conjugation FITC
Immunogen Rat IgG whole molecule.

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, FITC, Purified

Applications

Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
The antibody can be used at 5 μg/ml.

Reactivities Mouse
Conjugation FITC

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, FITC

Applications Flow Cytometry: Use 50µl of 5µg/ml diluted antibody to label 10e6 cells in 100µl.
Reactivities Mouse
Conjugation FITC

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, FITC

Applications Flow Cytometry: Use 10 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-3, FITC

Applications Flow Cytometry: 5 µg/ml, use 50 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Bovine IgG sheep polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2,000.
Reactivities Bovine
Conjugation FITC
Immunogen Purified Bovine IgG.

Bovine IgM sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on frozen sections: 1/200-1/2000.
Reactivities Bovine
Conjugation FITC
Immunogen Purified Bovine IgM

Bovine IgG1 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC
Immunogen Purified bovine IgG1

Bovine IgG2 sheep polyclonal antibody, FITC

Applications Flow Cytometry: 1/20 - 1/100.
Immunohistochemistry on frozen sections: 1/20 - 1/100.
Reactivities Bovine
Conjugation FITC
Immunogen Purified bovine IgG2

Canine IgA goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200-1/2000.
Reactivities Canine
Conjugation FITC
Immunogen Purified Canine IgA.

Canine IgM goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections:
1/200-1/2000.
Reactivities Canine
Conjugation FITC
Immunogen Purified Canine IgM.

Equine IgG goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on Frozen Sections: 1/20-1/100.
Reactivities Equine
Conjugation FITC
Immunogen Purified equine IgG (T).

Equine IgM goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on Frozen Sections: 1/100-1/1000.
Reactivities Equine
Conjugation FITC
Immunogen Purified equine IgM

Feline IgG (Fc specific) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections: 1/200-1/2,000.
Reactivities Feline
Conjugation FITC
Immunogen Purified Feline IgG Fc fragment.

Feline IgM goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Reactivities Feline
Conjugation FITC
Immunogen Purified Feline IgM

Porcine IgG F(c) specific goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/20-1/100.
Immunohistochemistry on Frozen Sections: 1/20-1/100.
Reactivities Porcine
Conjugation FITC
Immunogen Purified Porcine IgG.

Chicken IgM goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200 - 1/2,000.
Reactivities Chicken
Conjugation FITC
Immunogen Purified chicken IgM

Chicken IgA goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1/200 - 1/2,000.
Reactivities Chicken
Conjugation FITC
Immunogen Purified chicken IgA

Chicken IgG / Chicken IgY (Fc specific) goat polyclonal antibody, FITC

Applications Flow cytometry.
Reactivities Chicken
Conjugation FITC
Immunogen Purified chicken IgG - Fc fragment

Guinea pig IgG (H+L chain) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1:200.
Reactivities Guinea Pig
Conjugation FITC

Porcine IgG (H+L chain) goat polyclonal antibody, FITC

Applications Flow Cytometry.
Immunohistochemistry on frozen sections: 1:200 - 1:2000.
Reactivities Porcine
Conjugation FITC