Secondary Antibodies

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Sheep IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/25,000.
Western Blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Sheep IgG whole molecule

Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using peroxidase conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:20,000 to 1:80,000 is suggested for this product.
Conjugation Biotin
Immunogen Sheep IgG whole molecule

Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation FITC
Immunogen Sheep IgG whole molecule.

Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA: 1/400,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Sheep IgG whole molecule.

Sheep IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
ELISA : 1 :20,000-1 :100,000
Western blot : 1 :2,000-1 :10,000
Immunohistochemistry : 1 :1,000-1 :5,000.
Immunogen Sheep IgG whole molecule

Sheep IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation TRITC
Immunogen Sheep IgG whole molecule.

Sheep IgG (H+L chain) rabbit polyclonal antibody, Texas Red

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Sheep IgG whole molecule

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot, 1/500-1/2,500), ELISA (1/3,000-1/12,000) and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Conjugation AP
Immunogen Mouse IgG and IgM whole molecules

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting (Western Blot, 1:2,000-1:10,000), ELISA (1:20,000-1:100,000), Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Conjugation Biotin
Immunogen Mouse IgG and IgM whole molecules

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy (1:500-1:2,500) and Flow cytometry (1:2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Mouse IgG and IgM whole molecules

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/40,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Mouse IgG and IgM whole molecules.

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/100,000
Western Blot: 1/5,000-1/20,000
Immunohistochemistry: 1/2,000-1/10,000
Immunogen Mouse IgG and IgM whole molecules

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy (1:500-1:2,500) and Flow cytometry (1:2,000-1:10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Mouse IgG and IgM whole molecules.

Mouse IgG (H+L chain) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry or FACS analysis (1/2,000-1/10,000) as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Mouse IgG whole molecule.

Mouse IgG (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Immunomicroscopy or Immunofluorescence and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Mouse IgG whole molecule.

Rat IgG (H+L chain) goat polyclonal antibody, HRP

Applications Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/300,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/1,500.
Conjugation HRP
Immunogen Rat IgG whole molecule

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications Suitable for immunoblotting (western or dot blot, 1:500- 1:2,000), ELISA (1:2,000-1:8,000) and Immunohistochemistry (1:200- 1:1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Conjugation AP
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin

Applications Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation FITC
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Chicken IgG whole molecule.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Chicken IgG whole molecule

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Texas Red

Applications Suitable for Imunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation Texas Red
Immunogen Chicken IgG whole molecule.

Hamster IgG goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:65,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Hamster IgG whole molecule.

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Biotin

Applications ELISA: 500ng/ml.
Reactivities Mouse
Conjugation Biotin

Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, Purified

Applications ELISA: 5 µg/ml as coating antibody.
Reactivities Mouse

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, Biotin

Applications ELISA: 0.5µg/ml - 1.0µg/ml.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-7, HRP

Applications ELISA: 0.5 µg/ml as coating antibody.
Reactivities Mouse
Conjugation HRP

Mouse IgG2b (heavy chain) rat monoclonal antibody, clone LO-MG2b-2, Biotin

Applications ELISA: 500 ng/ml as a detection antibody.
Reactivities Mouse
Conjugation Biotin

Mouse IgG2b (heavy chain) rat monoclonal antibody, clone LO-MG2b-2, FITC

Applications Flow Cytometry: Use 10 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

Mouse IgG2b (heavy chain) rat monoclonal antibody, clone LO-MG2b-2, Purified

Applications ELISA: 1.25 µg/ml as a detection antibody.
Reactivities Mouse

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, Biotin

Applications ELISA: 0.5-1.0 µg/ml.
Reactivities Mouse
Conjugation Biotin

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, Purified

Applications ELISA: 5 µ/ml as a coating antibody.
Reactivities Mouse

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, Biotin

Applications ELISA (Use at  0.5 µg/ml as a detection antibody, avidity 3 x 10e9 M-1).
Reactivities Mouse
Conjugation Biotin

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, FITC

Applications Useful as second antibody in Immunoassays, such as ELISA's
Reactivities Mouse
Conjugation FITC

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, HRP

Applications ELISA: 500ng/ml.
Reactivities Mouse
Conjugation HRP

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, Purified

Applications ELISA: 5 µg/ml as capture antibody.
Reactivities Mouse

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, Biotin

Applications ELISA: 250ng/ml.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (heavy chain) rat monoclonal antibody, clone LO-MM-9, Purified

Applications ELISA: 5ug/ml (as a coating antibody).
Immunohistochemistry on frozen sections.
Reactivities Mouse

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Biotin

Applications ELISA: 500 ng/ml.
Reactivities Rat
Conjugation Biotin

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, FITC

Applications Flow cytometry (5 µg/ml): use 50 µl of the suggested working dilution to label
10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgD (heavy chain) mouse monoclonal antibody, clone MARD-3, Biotin

Applications ELISA: 500 ng/ml.
Reactivities Rat
Conjugation Biotin

Rat IgE mouse monoclonal antibody, clone MARE-1, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 2 x 10e7 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, Biotin

Applications ELISA: 500ng/ml.
Reactivities Rat
Conjugation Biotin

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, HRP

Applications ELISA: 0.5 µg/ml.
Reactivities Rat
Conjugation HRP

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, Purified

Applications ELISA: 3-5 µg/ml.
This antibody may be used as a Coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified Rat IgG1 (Cat.-No SA013).
Reactivities Rat

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, Biotin

Applications ELISA: 500ng/ml.
Reactivities Rat
Conjugation Biotin

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, HRP

Applications ELISA: 0.5µg/ml.
Reactivities Rat
Conjugation HRP

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, Purified

Applications ELISA: 1.25 µg/ml; This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgA (SA021).
Reactivities Rat