Secondary Antibodies

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Canine IgA (Fc specific) Goat Polyclonal Antibody
AP31539HR-N

USD 410.00

2 Weeks

Canine IgA (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (Fc specific) Goat Polyclonal Antibody
AP31540HR-N

USD 410.00

2 Weeks

Canine IgG (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in Dog serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/2000-1/10000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Dog serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgG (H+L chain) Goat Polyclonal Antibody
AP31541HR-N

USD 290.00

2 Weeks

Canine IgG (H+L chain) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in dog serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/10000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Canine IgM (Fc specific) Goat Polyclonal Antibody
AP31542HR-N

USD 550.00

2 Weeks

Canine IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000.
Reactivities Canine
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543HR-N

USD 550.00

2 Weeks

Mouse IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/400.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/8000 depending on the method used.
Reactivities Mouse
Conjugation HRP
Immunogen Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure.
Chicken IgG / Chicken IgY (H+L chain) Goat Polyclonal Antibody
AP31795HR-N

USD 260.00

2 Weeks

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP

Applications Sandwich ELISA: 1/120,000 dilution of antibody was found to generate an O.D of 1.0 in a 15 minute reaction with Tetramethyl Benzidine as the substrate.
Western blot (1/5000).
Immunoelectrophoresis.
Reactivities Chicken
Conjugation HRP
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.
Mouse IgM (Fc specific) Rabbit Polyclonal Antibody
AP32992HR-N

USD 550.00

2 Weeks

Mouse IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections. 
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions 
Histochemical and cytochemical Use: 1/100- 1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000- 1/5,000.
Reactivities Mouse
Conjugation HRP
Immunogen Purified homogenous IgM isolated from Mouse serum. Immunization with intact and split IgM.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Bovine IgG (H+L chain) Rabbit Polyclonal Antibody
R1304HRP

USD 255.00

3 Days

Bovine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western blot: 1/1,000-1/10,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP
Immunogen Bovine IgG whole molecule.
Rat IgG (H+L chain) Rabbit Polyclonal Antibody
R1371HRP

USD 240.00

3 Days

Rat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidas antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/170,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,500.
Conjugation HRP
Immunogen Rat IgG whole molecule
Peroxidase Conjugated Goat Anti-Mouse IgM (u-chain specific)
TA130007

USD 225.00

In Stock

Peroxidase Conjugated Goat Anti-Mouse IgM (u-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Mouse IgM
Conjugation HRP
Immunogen Mouse IgM, whole molecule
Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)
TA130032

USD 295.00

3 Weeks

Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Goat IgG
Conjugation HRP
Immunogen Goat IgG, whole molecule
Sheep/Goat IgG Donkey Polyclonal Antibody
AP05395HR-N

USD 735.00

5 Days

Sheep/Goat IgG donkey polyclonal antibody, HRP

Applications ELISA: 1/12800-1/25600.
Western Blot: 1/20000.
Immunohistochemistry on Paraffin Sections.
Immunohistochemistry on Frozen Sections: 1/50-1/100.
Reactivities Goat, Sheep
Conjugation HRP
Immunogen Sheep IgG
Chicken IgA (Fc specific) Goat Polyclonal Antibody
AP21442HR-N

USD 410.00

2 Weeks

Chicken IgA (Fc specific) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in chicken serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Immunocytochemistry: 1/50-1/250.
Immunohistochemistry: 1/50-1/250.
Reactivities Chicken
Conjugation HRP
Immunogen Purified normal IgA isolated from pooled Chicken serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Goat IgG (H+L chain) Chicken Polyclonal Antibody
AP31796HR-N

USD 260.00

2 Weeks

Goat IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis.
Reactivities Goat
Conjugation HRP
Immunogen Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant.
Production: After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase.
Porcine IgM (Fc specific) Goat Polyclonal Antibody
AP33049HR-N

USD 550.00

2 Weeks

Porcine IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used:
• In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen using a reference antibody of swine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
• In non-isotopic assay methodology (e.g. ELISA) to measure IgM in swine serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Porcine
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Duck IgG (H+L chain) Rabbit Polyclonal Antibody
AP33057HR-N

USD 290.00

2 Weeks

Duck IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA.
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
In enzyme-immunocytochemical and immunohistochemical staining for the detection of duck IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In nonisotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in duck serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Reactivities Duck
Conjugation HRP
Immunogen Purified normal Ig fractions isolated from pooled Duck serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Guinea pig IgG (H+L chain) Rabbit Polyclonal Antibody
R1322HRP

USD 255.00

3 Days

Guinea pig IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Note: This product has been assayed against 1.0 µg of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions:
ELISA: 1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Guinea Pig
Conjugation HRP
Immunogen Guinea Pig IgG whole molecule.
Monkey IgG (gamma chain specific) Goat Polyclonal Antibody
R1344HRP

USD 330.00

3 Days

Monkey IgG (gamma chain specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/50,000-1/150,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Monkey IgG gamma heavy chain.
Mouse Kappa Light Chain Rabbit Polyclonal Antibody
R1357HRP

USD 340.00

3 Days

Mouse Kappa Light Chain rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (Western or dot blot), ELISA, immuno-peroxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western blot: 1/2,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Mouse k (kappa) light chain
Mouse lambda light chain Rabbit Polyclonal Antibody
R1358HRP

USD 340.00

3 Days

Mouse lambda light chain rabbit polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/50,000 .
Western Blot: 1/1,000 - 1/5,000.
Immunohistochemistry on paraffin sections: 1/500 - 1/2,500.
Conjugation HRP
Immunogen Mouse lambda light chain
Peroxidase Conjugated Rabbit Anti-human IgG (gamma-chain specific)
TA130050

USD 367.00

3 Weeks

Peroxidase Conjugated Rabbit Anti-human IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Human IgG
Conjugation HRP
Immunogen Human IgG, whole molecule
Rat IgG2a Goat Polyclonal Antibody
AP05379HR-N

USD 310.00

5 Days

Rat IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/10,000.
Western Blot: 1/1,000-1/10,000.
Reactivities Rat
Conjugation HRP
Mouse IgG2a Goat Polyclonal Antibody
AP05385HR-N

USD 310.00

5 Days

Mouse IgG2a goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000.
Western Blotting.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG2a paraproteins
Mouse IgG2b Goat Polyclonal Antibody
AP05386HR-N

USD 310.00

5 Days

Mouse IgG2b goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000. 
Western Blot. 
Immunohistochemistry on Frozen Sections
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG2b paraproteins
Mouse IgA (alpha chain specific) Goat Polyclonal Antibody
AP32277HR-N

USD 230.00

3 Days

Mouse IgA (alpha chain specific) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.
Guinea Pig IgG (Fc specific) Sheep Polyclonal Antibody
AP33060HR-N

USD 550.00

2 Weeks

Guinea Pig IgG (Fc specific) sheep polyclonal antibody, HRP

Applications ELISA.
Dot blot. 
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in guinea pig serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Reactivities Guinea Pig
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled guinea pig serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgG (Fc specific) Rabbit Polyclonal Antibody
AP33081HR-N

USD 550.00

2 Weeks

Sheep IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.  
Immunohistochemistry on Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/8.000.
Reactivities Sheep
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgG (H+L chain) Rabbit Polyclonal Antibody
AP33082HR-N

USD 290.00

2 Weeks

Sheep IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in sheep serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10.000.
Reactivities Sheep
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgM (Fc specific) Rabbit Polyclonal Antibody
AP33083HR-N

USD 550.00

2 Weeks

Sheep IgM (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of sheep origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in sheep serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/50-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Reactivities Sheep
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Sheep IgM (H+L chain) Rabbit Polyclonal Antibody
AP33084HR-N

USD 410.00

2 Weeks

Sheep IgM (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/250. 
ELISA and comparable non-precipitating antibody-binding assays: 1/2,000-1/10,000.
Reactivities Sheep
Conjugation HRP
Immunogen Purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Porcine IgG (Fc specific) Rabbit Polyclonal Antibody
AP33086HR-N

USD 550.00

2 Weeks

Porcine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Porcine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Porcine IgG (H+L chain) Rabbit Polyclonal Antibody
AP33087HR-N

USD 290.00

2 Weeks

Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA. 
Dot blot.
Immunoblotting.
Immunocytochemistry.
Immunohistochemistry Paraffin Sections.
Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in swine serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electrondense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemistry and Cytochemical Use: 1/100-1/500. 
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Reactivities Porcine
Conjugation HRP
Immunogen Purified normal IgG isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Bovine IgG (Fc specific) Rabbit Polyclonal Antibody
R1305HRP

USD 310.00

3 Days

Bovine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot 1:1,000 - 1:10,000), ELISA (1:10,000 - 1:50,000=, immunoperoxidase electron microscopy and immunohistochemistry (1:500 - 1;2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP
Immunogen Bovine IgG F(c) fragment
Bovine IgG (F(ab)2 specific) Rabbit Polyclonal Antibody
R1306HRP

USD 305.00

3 Days

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1:1,000-10,000), ELISA (1:10,000 - 1:50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1:500-1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP
Immunogen Bovine IgG F(ab')2 fragment.
Feline IgG (H+L chain) Rabbit Polyclonal Antibody
R1307HRP

USD 240.00

3 Days

Feline IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:16,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Cat IgG whole molecule.
Guinea pig IgG (H+L chain), adsorbed Goat Polyclonal Antibody
R1325HRP

USD 350.00

3 Days

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/100,000-1/200,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Guinea Pig IgG whole molecule.
Bovine IgG (H+L chain) Goat Polyclonal Antibody
R1616HRP

USD 250.00

3 Days

Bovine IgG (H+L chain) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/5,000-1/100,000, Western or dot blot), ELISA (1/10,000-1/200,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/500-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation HRP
Immunogen Bovine IgG whole molecule.
Mouse IgG (H+L chain), adsorbed Goat Polyclonal Antibody
SP1041HRP

USD 330.00

5 Days

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/500-1/1000.
Immunohistochemistry on Frozen Sections: 1/50.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG
Peroxidase Conjugated Goat Anti-human IgA (alpha-chain specific)
TA130049

USD 317.00

3 Weeks

Peroxidase Conjugated Goat Anti-human IgA (alpha-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Human IgA
Conjugation HRP
Immunogen Human IgA, whole molecule
Mouse IgG1 Goat Polyclonal Antibody
AP05384HR-N

USD 310.00

5 Days

Mouse IgG1 goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry onParaffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG1 paraproteins.
Mouse IgA Goat Polyclonal Antibody
AP05389HR-N

USD 310.00

5 Days

Mouse IgA goat polyclonal antibody, HRP

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA: 1/4000 - 1/8000.
Western Blot.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgA paraproteins
Human IgG (H+L chain) Chicken Polyclonal Antibody
AP31797HR-N

USD 260.00

2 Weeks

Human IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/300,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethyl-benzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and ELISA analysis.
Reactivities Human
Conjugation HRP
Immunogen Purified, non-denatured Human IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase.
Mouse IgG (H+L chain) Chicken Polyclonal Antibody
AP31798HR-N

USD 250.00

2 Weeks

Mouse IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/70,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot: 1/200 -1/5000.
Immunocytochemistry.
Immunohistochemistry: 1/250 - 1/5000.
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Mouse
Conjugation HRP
Immunogen Purified, non-denatured Mouse IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Horseradish peroxidase.
Rabbit IgG (H+L chain) Chicken Polyclonal Antibody
AP31799HR-N

USD 260.00

2 Weeks

Rabbit IgG (H+L chain) chicken polyclonal antibody, HRP

Applications ELISA: a 1/20,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate.
Western Blot (1/5000).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose gels and ELISA analysis.
Reactivities Rabbit
Conjugation HRP
Immunogen Purified, non-denatured Rabbit IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Rabbit IgG's were affinity-purified over a column conjugated with purified Rabbit IgG (containing both heavy and light chains) and then conjugated to Horseradish Peroxidase.
Mouse IgG3 Goat Polyclonal Antibody
AP32074HR-N

USD 310.00

5 Days

Mouse IgG3 goat polyclonal antibody, HRP

Applications

ELISA: 1/4000-1/8000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG3 paraproteins.
Mouse IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP32278HR-N

USD 210.00

3 Days

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.
Mouse IgG Goat Polyclonal Antibody
AP33451HR-N

USD 165.00

2 Weeks

Mouse IgG goat polyclonal antibody, HRP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP
Immunogen Purified Mouse IgG fraction
Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) Goat Polyclonal Antibody
AP33463HR-N

USD 305.00

3 Days

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, HRP, Aff - Purified

Applications Mouse IgG1 Peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/20,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation HRP
Immunogen Native Mouse IgG1 heavy chain Protein.
Chicken IgG / Chicken IgY (H+L chain) Rabbit Polyclonal Antibody
R1300HRP

USD 255.00

3 Days

Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
Western blot: 1/1,000-1/10,000.
ELISA: 1/10,000-1/50,000.
Immunohistochemistry: 1/500-1/2500.
Conjugation HRP
Immunogen Chicken IgY/IgG whole molecule
Canine IgG (Fc specific) Rabbit Polyclonal Antibody
R1311HRP

USD 315.00

3 Days

Canine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Dog IgG F(c) fragment