ORF28 (NC_001348) Virus Tagged ORF Clone

CAT#: VC100969

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 3], codon optimized for human cell expression, NP_040151

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100969 represents NCBI reference of NP_040151 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATTCGGACAGGTTTCTGCAATCCTTTCCTTACCCAGGCCAGCGGTATAAAATACAACCCTCGAA
  CTGGACGCGGTAGCAATCGGGAATTCCTTCACTCCTATAAGACTACGATGAGCTCTTTTCAGTTTTTGGC
  CCCCAAGTGCCTCGACGAGGATGTACCCATGGAAGAGAGAAAGGGGGTTCATGTGGGGACTCTCAGCCGC
  CCACCTAAAGTATATTGCAACGGGAAGGAGGTCCCAATCCTCGACTTTAGATGCAGCTCACCTTGGCCTC
  GGCGCGTGAACATCTGGGGGGAGATCGATTTTCGCGGAGATAAGTTTGACCCCCGGTTTAACACCTTCCA
  CGTCTATGACATCGTGGAAACTACAGAGGCGGCCTCCAACGGGGATGTATCAAGATTTGCCACTGCCACA
  AGGCCACTGGGAACTGTTATTACCCTCTTGGGGATGAGCAGGTGCGGCAAGCGGGTGGCCGTGCATGTTT
  ACGGCATTTGTCAATACTTTTATATTAACAAGGCCGAAGTGGATACCGCCTGTGGCATTAGGAGTGGGAG
  CGAGCTGTCTGTGCTCCTGGCAGAATGTCTGAGGTCCTCTATGATAACGCAGAACGACGCGACCCTCAAT
  GGTGACAAGAATGCATTCCACGGCACTAGCTTCAAATCAGCCAGTCCTGAATCTTTCCGGGTAGAGGTAA
  TTGAGCGGACCGACGTTTATTACTACGATACGCAACCGTGCGCATTTTATCGAGTGTACTCACCAAGCAG
  CAAATTTACTAACTACTTGTGTGATAATTTCCACCCTGAATTGAAAAAATATGAAGGAAGAGTAGATGCC
  ACCACAAGGTTTCTGATGGATAACCCTGGTTTTGTCAGTTTTGGATGGTACCAGCTCAAGCCCGGCGTGG
  ATGGCGAGAGGGTCAGAGTCCGACCCGCTTCACGACAGCTGACACTCAGCGATGTAGAGATTGATTGCAT
  GTCTGATAATCTCCAGGCCATCCCCAACGACGATAGCTGGCCCGACTATAAACTTCTCTGTTTTGACATT
  GAGTGCAAGAGTGGGGGCTCCAATGAGCTGGCCTTCCCTGACGCCACACATTTGGAGGACCTGGTGATAC
  AGATCAGTTGCCTGTTGTATTCCATCCCACGGCAGAGCCTCGAGCACATCCTTCTGTTTTCCCTGGGTAG
  TTGTGATCTCCCACAGCGATATGTCCAGGAAATGAAAGATGCTGGGCTCCCTGAGCCAACTGTCTTGGAG
  TTCGACTCAGAGTTCGAACTTCTGATCGCGTTCATGACACTGGTCAAACAGTATGCACCTGAATTCGCGA
  CGGGCTACAACATTGTTAACTTTGACTGGGCCTTCATTATGGAAAAACTCAATAGCATTTACAGCCTGAA
  ATTGGATGGCTATGGCAGTATCAACCGAGGGGGATTGTTTAAAATCTGGGACGTGGGAAAGTCTGGGTTT
  CAACGAAGGTCAAAGGTGAAGATTAACGGACTGATCTCACTGGATATGTATGCTATAGCAACAGAAAAAC
  TGAAGTTGTCTTCTTATAAACTTGATAGCGTGGCTCGCGAAGCGCTGAACGAGTCTAAACGGGACCTTCC
  CTACAAAGACATCCCCGGATACTATGCAAGTGGCCCCAACACCAGGGGGATCATTGGCGAGTACTGTATC
  CAGGACTCCGCACTTGTCGGCAAGCTGTTCTTCAAGTACCTGCCACACCTCGAGCTGTCTGCCGTTGCCA
  GGCTGGCCAGAATCACATTGACAAAGGCCATCTATGATGGGCAACAGGTTAGGATCTATACTTGTCTGTT
  GGGTCTCGCATCTAGTAGGGGATTTATCCTGCCTGACGGGGGCTACCCAGCTACTTTTGAGTACAAAGAT
  GTGATCCCAGATGTCGGAGACGTCGAAGAGGAAATGGACGAGGACGAATCCGTGTCCCCTACCGGGACCT
  CCTCTGGCAGGAACGTGGGTTACAAGGGAGCTAGGGTGTTTGATCCCGATACCGGCTTTTATATCGATCC
  TGTGGTGGTACTGGACTTTGCTTCCCTTTACCCTTCCATAATCCAAGCACACAATCTTTGCTTTACCACC
  CTCACTCTTAACTTCGAGACTGTGAAACGCCTGAATCCCAGCGACTACGCAACGTTTACTGTGGGCGGAA
  AAAGACTGTTCTTCGTGCGGTCTAATGTTCGCGAAAGTTTGCTCGGTGTACTCCTGAAAGACTGGCTGGC
  TATGCGAAAGGCGATTAGAGCACGGATCCCCGGCTCTAGCAGCGACGAGGCTGTACTGCTCGACAAGCAG
  CAGGCCGCTATCAAGGTAGTATGTAATAGTGTGTATGGTTTTACGGGTGTTGCGCAGGGTTTTTTGCCTT
  GTCTCTACGTGGCCGCCACCGTCACTACGATTGGTAGACAGATGCTGCTTTCCACAAGAGACTATATCCA
  TAATAACTGGGCTGCCTTCGAAAGGTTCATTACCGCCTTCCCAGATATCGAGTCAAGCGTGCTTTCCCAA
  AAGGCCTATGAAGTGAAGGTGATCTACGGTGATACCGATAGCGTGTTCATTCGCTTCAAAGGTGTGAGTG
  TGGAGGGTATTGCAAAGATTGGGGAAAAGATGGCACATATAATCTCAACAGCACTGTTCTGCCCACCGAT
  AAAACTGGAGTGCGAAAAAACATTCATCAAGCTCCTGCTGATAACTAAGAAAAAGTATATTGGAGTGATC
  TATGGAGGCAAGGTGCTTATGAAAGGGGTAGACCTCGTCCGCAAGAACAATTGCCAATTTATTAACGACT
  ACGCCCGCAAACTCGTGGAGCTCCTTCTGTATGACGATACAGTGTCCCGCGCAGCGGCTGAAGCTTCTTG
  CGTGAGTATAGCTGAGTGGAATAGAAGAGCGATGCCTAGTGGTATGGCCGGCTTCGGACGGATTATTGCT
  GATGCCCATAGGCAGATAACTTCTCCCAAACTGGACATCAACAAGTTCGTGATGACAGCAGAGCTCTCTC
  GACCGCCCTCTGCATATATCAATAGGCGGCTCGCACACCTGACTGTCTATTATAAACTTGTAATGCGGCA
  AGGCCAGATTCCTAATGTCAGGGAGCGAATTCCCTATGTGATCGTGGCTCCCACCGATGAAGTGGAGGCT
  GATGCAAAATCAGTCGCTCTGCTCCGGGGTGACCCCTTGCAGAACACTGCCGGGAAAAGGTGCGGGGAAG
  CCAAGAGGAAGCTGATCATCAGCGACTTGGCCGAGGATCCCATCCACGTAACAAGTCACGGCTTGTCACT
  CAATATTGATTATTATTTTAGTCATCTTATCGGTACTGCTTCCGTGACTTTTAAGGCACTGTTCGGGAAC
  GATACCAAACTGACAGAGCGACTTCTGAAACGCTTCATACCAGAGACCAGAGTGGTTAACGTCAAGATGC
  TGAACAGACTTCAGGCTGCTGGGTTCGTCTGTATCCACGCCCCATGTTGGGACAATAAAATGAACACTGA
  GGCAGAAATAACTGAAGAGGAACAGTCTCACCAGATAATGCGAAGGGTATTCTGTATACCCAAGGCAATC
  CTGCACCAGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100969 representing NP_040151
  Red=Cloning sites Green=Tags
  
  MAIRTGFCNPFLTQASGIKYNPRTGRGSNREFLHSYKTTMSSFQFLAPKCLDEDVPMEERKGVHVGTLSR
  PPKVYCNGKEVPILDFRCSSPWPRRVNIWGEIDFRGDKFDPRFNTFHVYDIVETTEAASNGDVSRFATAT
  RPLGTVITLLGMSRCGKRVAVHVYGICQYFYINKAEVDTACGIRSGSELSVLLAECLRSSMITQNDATLN
  GDKNAFHGTSFKSASPESFRVEVIERTDVYYYDTQPCAFYRVYSPSSKFTNYLCDNFHPELKKYEGRVDA
  TTRFLMDNPGFVSFGWYQLKPGVDGERVRVRPASRQLTLSDVEIDCMSDNLQAIPNDDSWPDYKLLCFDI
  ECKSGGSNELAFPDATHLEDLVIQISCLLYSIPRQSLEHILLFSLGSCDLPQRYVQEMKDAGLPEPTVLE
  FDSEFELLIAFMTLVKQYAPEFATGYNIVNFDWAFIMEKLNSIYSLKLDGYGSINRGGLFKIWDVGKSGF
  QRRSKVKINGLISLDMYAIATEKLKLSSYKLDSVAREALNESKRDLPYKDIPGYYASGPNTRGIIGEYCI
  QDSALVGKLFFKYLPHLELSAVARLARITLTKAIYDGQQVRIYTCLLGLASSRGFILPDGGYPATFEYKD
  VIPDVGDVEEEMDEDESVSPTGTSSGRNVGYKGARVFDPDTGFYIDPVVVLDFASLYPSIIQAHNLCFTT
  LTLNFETVKRLNPSDYATFTVGGKRLFFVRSNVRESLLGVLLKDWLAMRKAIRARIPGSSSDEAVLLDKQ
  QAAIKVVCNSVYGFTGVAQGFLPCLYVAATVTTIGRQMLLSTRDYIHNNWAAFERFITAFPDIESSVLSQ
  KAYEVKVIYGDTDSVFIRFKGVSVEGIAKIGEKMAHIISTALFCPPIKLECEKTFIKLLLITKKKYIGVI
  YGGKVLMKGVDLVRKNNCQFINDYARKLVELLLYDDTVSRAAAEASCVSIAEWNRRAMPSGMAGFGRIIA
  DAHRQITSPKLDINKFVMTAELSRPPSAYINRRLAHLTVYYKLVMRQGQIPNVRERIPYVIVAPTDEVEA
  DAKSVALLRGDPLQNTAGKRCGEAKRKLIISDLAEDPIHVTSHGLSLNIDYYFSHLIGTASVTFKALFGN
  DTKLTERLLKRFIPETRVVNVKMLNRLQAAGFVCIHAPCWDNKMNTEAEITEEEQSHQIMRRVFCIPKAI
  LHQS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 3582 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001348.1, NP_040151
• RefSeq ORF: 3582
• Locus ID: 1487712
• MW: 134.0 kDa