ORF64 (NC_001348) Virus Tagged ORF Clone

CAT#: VC101010

Myc-DDK-tagged ORF clone of viral ORF for virion protein US10 [Human herpesvirus 3], codon optimized for human cell expression, NP_040191

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF64
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101010 represents NCBI reference of NP_040191 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTGTGCGGCTCTCGCGGCGAGCACCCCGGGGGGGAGTACGCTGGCCTCTACTGCACCAGACATG
  ACACCCCAGCGCATCAGGCCCTCATGAACGACGCGGAGCGATATTTCGCTGCAGCCCTCTGTGCCATCTC
  AACTGAAGCCTACGAAGCATTCATCCACTCACCTTCCGAGCGCCCTTGCGCATCACTTTGGGGGCGCGCT
  AAAGACGCCTTTGGACGCATGTGTGGGGAGCTTGCAGCTGATCGGCAGCGGCCGCCCTCTGTGCCCCCAA
  TCAGAAGAGCAGTATTGAGTCTTCTCCGGGAGCAATGCATGCCTGACCCTCAGTCCCATCTTGAATTGTC
  AGAACGACTGATACTCATGGCTTACTGGTGTTGTCTGGGACACGCTGGCCTTCCTACCATTGGGTTGTCT
  CCTGACAATAAATGTATACGCGCCGAGCTCTACGACAGACCCGGAGGAATCTGCCACCGCCTGTTTGACG
  CCTACTTGGGATGCGGATCTCTCGGCGTGCCAAGGACCTACGAGCGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101010 representing NP_040191
  Red=Cloning sites Green=Tags
  
  MNLCGSRGEHPGGEYAGLYCTRHDTPAHQALMNDAERYFAAALCAISTEAYEAFIHSPSERPCASLWGRA
  KDAFGRMCGELAADRQRPPSVPPIRRAVLSLLREQCMPDPQSHLELSERLILMAYWCCLGHAGLPTIGLS
  PDNKCIRAELYDRPGGICHRLFDAYLGCGSLGVPRTYERS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 540 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001348.1, NP_040191
• RefSeq ORF: 540
• Locus ID: 1487710
• MW: 19.9 kDa