ORF42 (NC_001348) Virus Tagged ORF Clone

CAT#: VC100984

Myc-DDK-tagged ORF clone of viral ORF for DNA packaging terminase subunit 1 [Human herpesvirus 3], codon optimized for human cell expression, NP_040165

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100984 represents NCBI reference of NP_040165 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTGATCATGTTCGGACGGACCCTCGGTGAGGAGTCTGTACGGTACTTCGAACGGCTGAAACGGA
  GAAGGGACGAGAGGTTTGGAACTCTCGAAAGTCCCACACCATGCTCTACAAGGCAAGGCAGCCTCGGCAA
  CGCTACCCAAATACCTTTCCTGAATTTTGCCATCGACGTGACCAGGCGCCACCAGGCCGTGATACCCGGA
  ATTGGGACTTTGCACAATTGCTGTGAGTACATTCCACTGTTCAGCGCAACAGCAAGACGGGCAATGTTTG
  GAGCGTTCTTGAGCAGTACCGGATATAACTGCACCCCTAACGTTGTCCTTAAGCCATGGAGATACTCTGT
  CAATGCAAACGTGTCACCTGAGTTGAAGAAGGCAGTATCCTCTGTCCAGTTCTATGAGTATAGCCCTGAG
  GAGGCTGCGCCTCATCGGAACGCCTATAGCGGAGTCATGAACACTTTTAGGGCCTTCTCCCTGTCTGACA
  GCTTCTGCCAGTTGAGCACTTTCACTCAGAGGTTCTCTTATCTGGTAGAAACTAGTTTTGAAAGCATCGA
  AGAGTGCGGGTCCCATGGTAAGCGGGCCAAGGTGGACGTGCCGATTTACGGAAGATATAAGGGAACCCTG
  GAACTGTTTCAGAAGATGATTCTCATGCACACTACCCATTTCATTAGTTCTGTCCTTCTGGGGGATCACG
  CCGATAGAGTGGACTGCTTTCTGAGGACTGTGTTTAATACACCCAGTGTGTCCGACAGTGTGCTGGAGCA
  TTTTAAACAGAAGTCAACTGTTTTCCTTGTGCCGAGAAGACATGGAAAGACTTGGTTCCTCGTCCCACTC
  ATCGCTCTTGTGATGGCTACGTTCCGCGGTATCAAGGTTGGCTATACCGCTCACATCCGAAAGGCAACGG
  AACCAGTGTTTGAGGGTATAAAGTCTCGCCTCGAGCAGTGGTTCGGAGCAAACTATGTAGACCACGTTAA
  AGGGGAATCTATCACCTTTAGCTTTACTGATGGCAGTTACTCTACAGCAGTGTTTGCAAGTAGTCATAAT
  ACTAACGGCATCCGCGGCCAGGATTTTAATCTGTTGTTCGTTGATGAAGCAAATTTTATCCGCCCTGACG
  CCGTACAAACAATTGTAGGGTTCCTGAACCAGACCAATTGTAAGATAATTTTCGTGTCCTCCACAAACAC
  TGGAAAGGCGTCCACCTCATTTCTTTATAACCTGCGCGGCTCTAGCGACCAGCTCCTGAATGTCGTCACC
  TACGTGTGCGACGATCATATGCCTCGGGTTCTCGCACACTCAGACGTTACGGCCTGCTCTTGCTACGTAC
  TGAACAAACCTGTCTTTATCACAATGGACGGTGCAATGCGGAGGACTGCCGATCTCTTCATGGCCGACTC
  CTTTGTGCAGGAGATCGTGGGAGGACGCAAACAAAACTCTGGCGGAGTGGGCTTCGATAGACCCCTTTTT
  ACCAAGACAGCCCGAGAACGGTTTATACTCTATAGACCTTCCACCGTAGCAAACTGTGCCATTCTGTCCA
  GTGTCCTGTATGTTTACGTGGATCCCGCTTTTACCTCCAATACCCGAGCAAGCGGGACAGGTGTAGCTAT
  CGTTGGTCGATATAAGTCAGATTGGATCATCTTCGGCCTCGAGCACTTTTTCCTCAGAGCTCTGACAGGC
  ACTAGCTCTTCTGAGATAGGCAGATGCGTTACCCAATGTCTGGGTCACATTCTTGCCCTTCACCCTAATA
  CTTTCACAAATGTGCATGTATCCATTGAAGGCAATAGTAGCCAAGACTCCGCTGTGGCGATTAGCTTGGC
  CATTGCACAGCAGTTTGCTGTATTGGAAAAGGGGAATGTGCTGTCTAGCGCGCCTGTGTTGTTGTTTTAC
  CACTCTATCCCCCCCGGATGTTCAGTGGCATATCCTTTTTTCTTGCTGCAGAAGCAGAAAACACCCGCAG
  TGGACTACTTTGTCAAGAGATTCAACAGCGGAAACATCATTGCTAGCCAGGAGCTCGTGTCTCTTACAGT
  AAAACTCGGTGTGGACCCTGTGGAGTATTTGTGTAAGCAGCTCGACAACCTGACGGAGGTGATCAAGGGC
  GGCATGGGCAACCTGGACACTAAAACTTACACGGGCAAAGGAACCACAGGTACTATGTCCGATGACCTGA
  TGGTTGCCCTCATCATGAGCGTGTATATAGGGTCAAGTTGTATCCCTGACAGCGTGTTTATGCCTATAAA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100984 representing NP_040165
  Red=Cloning sites Green=Tags
  
  MSLIMFGRTLGEESVRYFERLKRRRDERFGTLESPTPCSTRQGSLGNATQIPFLNFAIDVTRRHQAVIPG
  IGTLHNCCEYIPLFSATARRAMFGAFLSSTGYNCTPNVVLKPWRYSVNANVSPELKKAVSSVQFYEYSPE
  EAAPHRNAYSGVMNTFRAFSLSDSFCQLSTFTQRFSYLVETSFESIEECGSHGKRAKVDVPIYGRYKGTL
  ELFQKMILMHTTHFISSVLLGDHADRVDCFLRTVFNTPSVSDSVLEHFKQKSTVFLVPRRHGKTWFLVPL
  IALVMATFRGIKVGYTAHIRKATEPVFEGIKSRLEQWFGANYVDHVKGESITFSFTDGSYSTAVFASSHN
  TNGIRGQDFNLLFVDEANFIRPDAVQTIVGFLNQTNCKIIFVSSTNTGKASTSFLYNLRGSSDQLLNVVT
  YVCDDHMPRVLAHSDVTACSCYVLNKPVFITMDGAMRRTADLFMADSFVQEIVGGRKQNSGGVGFDRPLF
  TKTARERFILYRPSTVANCAILSSVLYVYVDPAFTSNTRASGTGVAIVGRYKSDWIIFGLEHFFLRALTG
  TSSSEIGRCVTQCLGHILALHPNTFTNVHVSIEGNSSQDSAVAISLAIAQQFAVLEKGNVLSSAPVLLFY
  HSIPPGCSVAYPFFLLQKQKTPAVDYFVKRFNSGNIIASQELVSLTVKLGVDPVEYLCKQLDNLTEVIKG
  GMGNLDTKTYTGKGTTGTMSDDLMVALIMSVYIGSSCIPDSVFMPIK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 2241 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001348.1, NP_040165
• RefSeq ORF: 2241
• Locus ID: 1487719
• MW: 82.8 kDa