ORF31 (NC_001348) Virus Tagged ORF Clone

CAT#: VC100972

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein B [Human herpesvirus 3], codon optimized for human cell expression, NP_040154

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100972 represents NCBI reference of NP_040154 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCATGCGGCTATTATTCCAAGTGGCGCAATAGAGACCGCCCCGAATACCGCCGGAACCTGAGAT
  TTCGCCGCTTCTTCAGCTCTATCCACCCTAACGCTGCCGCCGGAAGCGGCTTCAACGGGCCAGGAGTGTT
  CATTACCTCCGTGACCGGAGTGTGGTTGTGCTTTCTTTGTATCTTTTCAATGTTTGTCACCGCAGTCGTG
  AGCGTCTCCCCCAGTTCATTTTATGAGAGTCTGCAGGTGGAGCCCACCCAGTCCGAAGACATAACTCGAA
  GTGCCCACTTGGGAGACGGCGATGAGATTAGGGAAGCAATTCACAAGTCACAGGATGCTGAAACTAAGCC
  AACATTTTACGTATGCCCGCCCCCTACTGGCTCTACCATCGTTCGACTTGAGCCGACCCGAACCTGCCCC
  GACTACCATCTGGGCAAAAACTTTACGGAAGGGATTGCCGTGGTTTACAAAGAGAATATCGCTGCCTACA
  AATTTAAAGCAACCGTCTATTACAAAGATGTGATTGTAAGTACCGCATGGGCCGGCTCCTCCTACACCCA
  GATTACGAATAGGTACGCTGATCGCGTGCCCATTCCAGTGTCAGAGATCACCGATACCATTGATAAATTC
  GGGAAATGCAGCTCAAAAGCTACATACGTGCGCAATAATCATAAGGTGGAAGCCTTTAACGAGGACAAGA
  ACCCACAAGATATGCCACTGATTGCTTCCAAGTACAATTCCGTTGGATCAAAGGCCTGGCATACAACCAA
  CGACACCTACATGGTTGCGGGTACGCCAGGGACTTATAGAACCGGGACAAGCGTGAACTGTATCATAGAG
  GAGGTCGAGGCTCGGAGTATCTTCCCCTACGACTCTTTTGGGCTGTCAACTGGAGACATTATTTACATGT
  CACCGTTTTTCGGCCTCCGGGATGGCGCCTACAGGGAGCACTCTAATTATGCCATGGATAGGTTTCATCA
  GTTTGAAGGCTATCGACAGAGAGACCTGGACACTCGAGCCTTGCTGGAGCCAGCCGCGCGAAATTTCCTC
  GTGACTCCACATTTGACCGTGGGCTGGAATTGGAAACCCAAGAGAACCGAGGTTTGTTCCCTCGTTAAGT
  GGCGCGAAGTCGAGGATGTGGTCAGGGACGAATACGCCCACAACTTTCGCTTTACAATGAAAACCCTGTC
  TACCACTTTCATCTCCGAAACCAATGAGTTCAACCTGAACCAGATTCACCTCAGCCAGTGTGTAAAGGAG
  GAGGCACGCGCGATCATCAACAGAATCTACACAACTAGATATAATTCCTCACATGTGCGGACCGGAGACA
  TTCAGACTTATCTGGCCCGCGGGGGATTCGTGGTGGTGTTCCAGCCTCTGCTCAGCAACTCACTGGCGAG
  ACTCTATCTGCAGGAGCTGGTCAGGGAAAATACTAATCATTCACCACAGAAACACCCTACTAGAAACACA
  AGGTCCCGAAGGTCTGTCCCCGTTGAGTTGAGGGCCAATAGAACCATTACCACAACGTCAAGTGTGGAGT
  TTGCCATGCTGCAGTTTACCTACGACCACATTCAAGAACACGTGAATGAGATGCTGGCTCGGATTAGTTC
  CAGCTGGTGTCAGCTGCAGAACCGGGAACGGGCTTTGTGGTCTGGGCTCTTTCCTATCAACCCCAGCGCT
  CTGGCTTCTACTATCCTGGACCAGCGGGTGAAGGCTCGAATACTTGGTGACGTCATCTCCGTCTCCAATT
  GCCCCGAACTGGGGTCCGACACCAGGATCATCCTGCAGAATTCAATGCGCGTCTCCGGAAGCACAACTCG
  GTGTTACTCCCGCCCCCTCATCTCTATAGTAAGTCTGAACGGATCAGGAACTGTGGAAGGACAGCTGGGA
  ACCGATAATGAACTTATCATGTCCAGGGACTTGCTGGAACCGTGCGTCGCAAATCACAAGAGATATTTCC
  TTTTTGGGCATCACTACGTTTATTACGAAGACTATCGGTACGTTCGGGAGATCGCAGTTCACGATGTCGG
  TATGATCTCCACATATGTCGACCTCAATCTTACCCTTTTGAAGGACAGAGAGTTTATGCCTCTGCAGGTC
  TACACTCGCGATGAACTCCGGGATACTGGCTTGCTCGACTACTCCGAGATTCAGAGGAGAAATCAAATGC
  ATAGCCTGAGGTTCTACGACATCGATAAGGTGGTGCAGTACGACTCAGGCACAGCTATCATGCAGGGGAT
  GGCACAGTTCTTCCAGGGACTTGGTACAGCTGGCCAGGCTGTAGGCCACGTAGTTTTGGGGGCAACGGGC
  GCCCTGCTGAGTACCGTCCACGGTTTTACAACCTTCCTGAGCAATCCCTTTGGAGCTCTCGCGGTTGGTC
  TGCTGGTACTCGCCGGCCTGGTGGCTGCCTTTTTTGCCTATCGGTATGTCCTTAAGCTGAAAACTTCACC
  TATGAAAGCCCTGTACCCCCTGACTACCAAGGGCCTTAAACAGCTGCCGGAGGGTATGGACCCCTTCGCG
  GAGAAGCCTAATGCTACCGACACACCAATAGAGGAGATCGGGGATAGTCAGAATACCGAACCCTCCGTCA
  ACTCAGGCTTCGATCCCGACAAATTCAGGGAAGCTCAGGAGATGATTAAATACATGACTCTGGTGTCCGC
  TGCCGAGCGGCAGGAGTCTAAAGCTCGGAAGAAAAACAAGACCAGTGCCCTCCTTACATCCCGGCTTACA
  GGATTGGCCCTTCGGAACAGGCGCGGCTATTCTCGCGTCCGGACCGAGAATGTAACAGGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100972 representing NP_040154
  Red=Cloning sites Green=Tags
  
  MSPCGYYSKWRNRDRPEYRRNLRFRRFFSSIHPNAAAGSGFNGPGVFITSVTGVWLCFLCIFSMFVTAVV
  SVSPSSFYESLQVEPTQSEDITRSAHLGDGDEIREAIHKSQDAETKPTFYVCPPPTGSTIVRLEPTRTCP
  DYHLGKNFTEGIAVVYKENIAAYKFKATVYYKDVIVSTAWAGSSYTQITNRYADRVPIPVSEITDTIDKF
  GKCSSKATYVRNNHKVEAFNEDKNPQDMPLIASKYNSVGSKAWHTTNDTYMVAGTPGTYRTGTSVNCIIE
  EVEARSIFPYDSFGLSTGDIIYMSPFFGLRDGAYREHSNYAMDRFHQFEGYRQRDLDTRALLEPAARNFL
  VTPHLTVGWNWKPKRTEVCSLVKWREVEDVVRDEYAHNFRFTMKTLSTTFISETNEFNLNQIHLSQCVKE
  EARAIINRIYTTRYNSSHVRTGDIQTYLARGGFVVVFQPLLSNSLARLYLQELVRENTNHSPQKHPTRNT
  RSRRSVPVELRANRTITTTSSVEFAMLQFTYDHIQEHVNEMLARISSSWCQLQNRERALWSGLFPINPSA
  LASTILDQRVKARILGDVISVSNCPELGSDTRIILQNSMRVSGSTTRCYSRPLISIVSLNGSGTVEGQLG
  TDNELIMSRDLLEPCVANHKRYFLFGHHYVYYEDYRYVREIAVHDVGMISTYVDLNLTLLKDREFMPLQV
  YTRDELRDTGLLDYSEIQRRNQMHSLRFYDIDKVVQYDSGTAIMQGMAQFFQGLGTAGQAVGHVVLGATG
  ALLSTVHGFTTFLSNPFGALAVGLLVLAGLVAAFFAYRYVLKLKTSPMKALYPLTTKGLKQLPEGMDPFA
  EKPNATDTPIEEIGDSQNTEPSVNSGFDPDKFREAQEMIKYMTLVSAAERQESKARKKNKTSALLTSRLT
  GLALRNRRGYSRVRTENVTGV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 2793 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001348.1, NP_040154
• RefSeq ORF: 2793
• Locus ID: 1487662
• MW: 105.3 kDa