ORF55 (NC_001348) Virus Tagged ORF Clone

CAT#: VC100996

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase helicase subunit [Human herpesvirus 3], codon optimized for human cell expression, NP_040177

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF55
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100996 represents NCBI reference of NP_040177 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGAAGCATCTCCGTGGATTCTTCCAGCCCCAAAAATGTATTTAACCCAGAGACCCCCAATGGGT
  TCGACGATAGCGTGTACCTGAACTTTACGAGCATGCACAGTATTCAGCCAATTCTCTCTAGGATCCGGGA
  GCTTGCAGCTATAACAATTCCCAAGGAGCGCGTACCAAGGCTCTGCTGGTTCAAGCAGCTGCTCGAGCTG
  CAAGCTCCTCCAGAGATGCAACGAAACGAGCTGCCATTCTCTGTTTACCTCATCAGCGGCAACGCTGGCT
  CCGGCAAATCTACCTGCATCCAAACCCTCAACGAGGCCATTGACTGTATCATAACAGGCTCCACCCGAGT
  GGCCGCACAGAATGTCCATGCCAAGCTGTCAACAGCTTACGCTAGCCGGCCGATCAATACTATTTTCCAC
  GAATTTGGATTCCGCGGAAATCACATCCAAGCCCAGCTGGGGAGGTATGCTTACAACTGGACTACTACGC
  CACCAAGCATCGAGGACTTGCAAAAGAGAGACATTGTGTATTATTGGGAAGTCCTGATTGACATCACCAA
  AAGGGTTTTTCAAATGGGCGACGATGGACGCGGCGGCACTAGTACTTTCAAGACGCTTTGGGCCATCGAA
  CGGCTGCTGAATAAGCCGACCGGATCTATGAGTGGAACCGCTTTTATCGCCTGTGGGAGCCTGCCCGCAT
  TTACCAGAAGTAACGTGATAGTGATTGACGAAGCTGGACTGCTTGGTCGACACATTCTGACAGCCGTAGT
  GTATTGCTGGTGGCTGCTGAACGCTATCTATCAGAGTCCACAATACATAAACGGAAGGAAGCCTGTCATC
  GTTTGTGTTGGCTCCCCCACCCAGACCGACTCACTTGAGTCACACTTCCAACATGATATGCAGCGGTCCC
  ATGTCACACCCAGTGAGAATATACTCACCTATATCATTTGTAATCAGACCCTCAGACAGTACACCAATAT
  TTCTCACAATTGGGCTATTTTTATCAATAACAAGAGATGTCAAGAGGATGACTTTGGGAACCTGCTGAAA
  ACTCTGGAATACGGGCTGCCAATTACGGAGGCCCATGCGAGACTCGTGGACACGTTCGTTGTCCCTGCAT
  CTTACATTAACAACCCTGCTAATTTGCCTGGCTGGACCCGGCTCTACAGCAGCCATAAAGAGGTATCTGC
  TTATATGAGTAAACTGCATGCCCATCTGAAGCTTAGCAAGAATGACCACTTTTCTGTGTTCGCGCTGCCA
  ACATACACTTTTATTCGCCTTACAGCCTTCGATGAATACCGCAAGCTGACAGGGCAACCCGGACTCTCAG
  TGGAACACTGGATCCGGGCAAACAGCGGCCGGCTGCATAACTATTCCCAGAGTAGGGATCATGATATGGG
  TACCGTGAAATATGAAACCCACTCCAACCGAGACTTGATTGTGGCACGCACTGATATCACATACGTGCTT
  AACTCACTTGTTGTCGTAACAACCCGCCTCAGGAAGCTCGTCATTGGGTTCTCAGGCACATTCCAATCAT
  TCGCCAAGGTGCTGCGGGATGATAGTTTTGTTAAAGCCAGGGGAGAGACCAGTATAGAGTACGCCTATCG
  CTTTCTGAGCAATCTGATCTTTGGGGGGCTGATTAACTTTTATAATTTTCTTCTGAACAAAAACCTCCAT
  CCGGATAAAGTCAGTCTGGCTTATAAACGACTGGCAGCACTTACACTCGAACTCCTGTCCGGGACTAATA
  AGGCACCCCTCCACGAGGCTGCTGTGAATGGCGCAGGTGCTGGCATCGATTGCGATGGGGCAGCCACCAG
  TGCCGATAAGGCTTTTTGTTTCACAAAAGCCCCTGAATCTAAGGTAACAGCTTCCATTCCCGAAGACCCT
  GATGACGTGATTTTTACAGCACTGAACGACGAAGTAATTGATCTCGTGTACTGTCAGTACGAATTTTCCT
  ACCCTAAGTCCAGCAACGAGGTCCATGCACAGTTTCTGCTGATGAAAGCCATCTACGACGGCCGGTATGC
  TATTTTGGCTGAGCTCTTTGAAAGCTCTTTTACCACAGCTCCATTCAGCGCTTACGTGGACAACGTGAAC
  TTCAATGGCAGCGAACTCTTGATTGGCAATGTAAGAGGCGGCTTGCTGAGCCTTGCGCTGCAGACCGACA
  CCTATACTCTGCTGGGCTACACGTTCGCGCCGGTACCCGTGTTTGTGGAGGAGCTCACACGCAAGAAGCT
  GTATAGGGAGACAACAGAAATGCTCTACGCCCTCCATGTGCCTTTGATGGTACTCCAGGACCAGCACGGC
  TTTGTTTCCATCGTTAACGCAAACGTATGCGAGTTCACAGAATCTATAGAAGACGCCGAGCTGGCAATGG
  CCACGACTGTCGATTATGGACTCTCATCCAAGCTGGCGATGACGATCGCCCGGTCCCAGGGCCTCTCCCT
  GGAGAAAGTGGCAATCTGCTTCACCGCGGATAAACTGCGGCTTAATTCTGTATATGTGGCCATGTCACGG
  ACAGTGTCCAGCCGATTCCTGAAGATGAATCTTAATCCACTGAGGGAAAGATATGAGAAGTCTGCCGAAA
  TTTCCGACCACATCCTCGCCGCCCTTAGGGATCCAAATGTCCATGTAGTGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100996 representing NP_040177
  Red=Cloning sites Green=Tags
  
  MKRSISVDSSSPKNVFNPETPNGFDDSVYLNFTSMHSIQPILSRIRELAAITIPKERVPRLCWFKQLLEL
  QAPPEMQRNELPFSVYLISGNAGSGKSTCIQTLNEAIDCIITGSTRVAAQNVHAKLSTAYASRPINTIFH
  EFGFRGNHIQAQLGRYAYNWTTTPPSIEDLQKRDIVYYWEVLIDITKRVFQMGDDGRGGTSTFKTLWAIE
  RLLNKPTGSMSGTAFIACGSLPAFTRSNVIVIDEAGLLGRHILTAVVYCWWLLNAIYQSPQYINGRKPVI
  VCVGSPTQTDSLESHFQHDMQRSHVTPSENILTYIICNQTLRQYTNISHNWAIFINNKRCQEDDFGNLLK
  TLEYGLPITEAHARLVDTFVVPASYINNPANLPGWTRLYSSHKEVSAYMSKLHAHLKLSKNDHFSVFALP
  TYTFIRLTAFDEYRKLTGQPGLSVEHWIRANSGRLHNYSQSRDHDMGTVKYETHSNRDLIVARTDITYVL
  NSLVVVTTRLRKLVIGFSGTFQSFAKVLRDDSFVKARGETSIEYAYRFLSNLIFGGLINFYNFLLNKNLH
  PDKVSLAYKRLAALTLELLSGTNKAPLHEAAVNGAGAGIDCDGAATSADKAFCFTKAPESKVTASIPEDP
  DDVIFTALNDEVIDLVYCQYEFSYPKSSNEVHAQFLLMKAIYDGRYAILAELFESSFTTAPFSAYVDNVN
  FNGSELLIGNVRGGLLSLALQTDTYTLLGYTFAPVPVFVEELTRKKLYRETTEMLYALHVPLMVLQDQHG
  FVSIVNANVCEFTESIEDAELAMATTVDYGLSSKLAMTIARSQGLSLEKVAICFTADKLRLNSVYVAMSR
  TVSSRFLKMNLNPLRERYEKSAEISDHILAALRDPNVHVVY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 2643 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001348.1, NP_040177
• RefSeq ORF: 2643
• Locus ID: 1487682
• MW: 98.8 kDa