ORF54 (NC_001348) Virus Tagged ORF Clone

CAT#: VC100995

Myc-DDK-tagged ORF clone of viral ORF for capsid portal protein [Human herpesvirus 3], codon optimized for human cell expression, NP_040176

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100995 represents NCBI reference of NP_040176 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGATCACCTCCCTGTTTAACAACTCTTCCGGGTCTGAAGAAAAGCGGATAGCCTCCTCCGTGT
  CAATCGACCAGGGGCTGAACGGAAGTAACCCAAATGATCAGTACAAAAATATGTTCGATATCTACTGGAA
  CGAGTATGCCCCAGATATTGGATTCTGTACCTTCCCTGAGGAAGATGGATGGATGTTGATTCATCCTACT
  ACCCAGAGCATGCTGTTTAGGAAGATCCTCGCAGGCGACTTTGGCTACACCGACGGCCAGGGCATTTACT
  CTGCCGTGAGGAGCACCGAAACCGTCATTCGGCAGGTGCAGGCTACCGTGCTCATGAATGCTCTGGACGC
  CACTCGATATGAGGATCTGGCCGCCGATTGGGAGCATCACATACAGCAGTGCAACCTCCACGCAGGAGCT
  CTGGCAGAGCGCTATGGCCTGTGTGGGGAATCAGAGGCAGTTCGGCTGGCGCATCAAGTATTTGAGACAT
  GGAGGCAGACCCTGCAGTCAAGCCTTCTGGAATTTCTTCGGGGCATCACAGGTTGTTTGTATACGAGTGG
  TCTTAACGGCCGCGTGGGGTTCGCCAAATACGTGGACTGGATTGCCTGCGTGGGTATCGTACCAGTGGTA
  CGCAAGGTGCGGAGTGAGCAAAATGGAACACCCGCCCCATTGAACACATATATGGGCCAAGCCGCCGAGC
  TGAGTCAGATGCTGAAGGTTGCAGATGCGACACTTGCCCGAGGTGCAGCAGTAGTGACATCCCTGGTAGA
  GTGTATGCAGAATGTTGCGATCATGGACTACGACCGGACGCGACTTTATTACAATTACAACAGGAGACTG
  ATTATGGCAAAAGATGATGTTACAGGGATGAAAGGTGAGTGTTTGGTGGTCTGGCCTCCTGTCGTCTGCG
  GCGAAGGCGTGGTCTTCGATTCACCCCTGCAGAGGCTGAGTGGCGAGGTGCTGGCGTGCTACGCCCTTAG
  AGAACACGCTAGAGTGTGCCAAGTCCTCAACACAGCCCCGCTGAGAGTGCTGATCGGGAGGAGGAACGAG
  GACGATCGAAGCCATAGTACAAGAGCAGTGGACCGAATCATGGGCGAGAACGACACTACCCGGGCCGGCT
  CAGCCGCATCTCGGCTGGTGAAACTTATCGTGAATCTGAAGAACATGCGGCACGTAGGGGACATTACCGA
  GACAGTGAGATCTTATCTCGAGGAGACGGGCAATCATATTCTGGAGGGATCAGGATCCGTGGATACGAGC
  CAGCCAGGATTCGGCAAGGCCAACCAAAGTTTCAATGGTGGAGCAATGAGTGGCACCACCAATGTTCAAA
  GCGCCTTTAAGACCAGCGTGGTTAATTCCATAAACGGAATGCTTGAGGGGTATGTGAACAACCTGTTCAA
  GACTATAGAGGGGCTGAAGGACGTCAACAGTGACCTGACAGAACGCCTGCAGTTTAAGGAGGGAGAACTC
  AAACGACTCAGGGAGGAGCGCGTGAAAATCAAACCATCTAAGGGCTCACACATCACAATGGCTGAGGAGA
  CCAGGATCGCCGATCTGAATCATGAAGTCATAGACCTCACCGGCATTATCGGTGACGATGCATACATTGC
  AAACTCATTCCAGAGCAGATACATCCCCCCTTATGGAGATGACATAAAGCGCCTGTCAGAGCTGTGGAAG
  CAGGAACTCGTAAGATGCTTCAAACTGCATCGAGTTAATAACAACCAGGGCCAAGAGATTAGCGTGTCCT
  ACTCCAACGCTTCCATCTCCCTGTTGGTCGCTCCATATTTCTCTTTTATCCTGCGAGCCACCCGGCTCGG
  CTTCCTGGTCACACAGAGCGAGGTCCACAGGTCAGAAGAGGAACTGTGCCAGGCTATCTTTAAAAAAGCC
  AGAACAGAATCTTATCTGAGCCAGATAAGAATCCTTTACGAGATGCAGGTTAGGGCAGAAGTTATTAAAC
  GGGGTCCACGGAGGACTCCATCTCCCTCATGGGGCTTGCCGGACCCCACCGAGGACGATGAGAGAATCCC
  TGAGCCCAACAAGATAAATAACCAGTATATGCACGTTGGCTATAAAAATCTGTCACATTTCATGAAGGGA
  CACCCCCCCGAGAGGCTGAGAGTGCACAAGGTGAACGCCGCCGACAGCACGCTTCTCGACAAAATTAGGG
  CTAATAGACGGCGCGGCGACGGCAGATGGGATGTGAGGAATAAGTACACCCAGCACTTTCGCTTGCAGAG
  AAACGACCGGCAGTTGACCAACACCAGCAGGCGCGGCGTAGGTTGCGAGCGAAGAGATAGGCGATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100995 representing NP_040176
  Red=Cloning sites Green=Tags
  
  MAEITSLFNNSSGSEEKRIASSVSIDQGLNGSNPNDQYKNMFDIYWNEYAPDIGFCTFPEEDGWMLIHPT
  TQSMLFRKILAGDFGYTDGQGIYSAVRSTETVIRQVQATVLMNALDATRYEDLAADWEHHIQQCNLHAGA
  LAERYGLCGESEAVRLAHQVFETWRQTLQSSLLEFLRGITGCLYTSGLNGRVGFAKYVDWIACVGIVPVV
  RKVRSEQNGTPAPLNTYMGQAAELSQMLKVADATLARGAAVVTSLVECMQNVAIMDYDRTRLYYNYNRRL
  IMAKDDVTGMKGECLVVWPPVVCGEGVVFDSPLQRLSGEVLACYALREHARVCQVLNTAPLRVLIGRRNE
  DDRSHSTRAVDRIMGENDTTRAGSAASRLVKLIVNLKNMRHVGDITETVRSYLEETGNHILEGSGSVDTS
  QPGFGKANQSFNGGAMSGTTNVQSAFKTSVVNSINGMLEGYVNNLFKTIEGLKDVNSDLTERLQFKEGEL
  KRLREERVKIKPSKGSHITMAEETRIADLNHEVIDLTGIIGDDAYIANSFQSRYIPPYGDDIKRLSELWK
  QELVRCFKLHRVNNNQGQEISVSYSNASISLLVAPYFSFILRATRLGFLVTQSEVHRSEEELCQAIFKKA
  RTESYLSQIRILYEMQVRAEVIKRGPRRTPSPSWGLPDPTEDDERIPEPNKINNQYMHVGYKNLSHFMKG
  HPPERLRVHKVNAADSTLLDKIRANRRRGDGRWDVRNKYTQHFRLQRNDRQLTNTSRRGVGCERRDRRS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 2307 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001348.1, NP_040176
• RefSeq ORF: 2307
• Locus ID: 1487723
• MW: 86.8 kDa