ORF47 (NC_001348) Virus Tagged ORF Clone

CAT#: VC100988

Myc-DDK-tagged ORF clone of viral ORF for tegument serine/threonine protein kinase [Human herpesvirus 3], codon optimized for human cell expression, NP_040169

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100988 represents NCBI reference of NP_040169 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCGACGACACCCCCCCAAACCTTCAAATCTCTCCCACCGCCGGCCCCCTGCGCAGTCACCACA
  ATACCGATGGCCACGAGCCCAACGCTACCGCCGCCGACCAACAAGAGCGGGAAAGTACGAATCCAACACA
  CGGATGTGTCAACCACCCTTGGGCTAACCCGAGCACTGCCACTTGTATGGAGAGCCCAGAGCGCAGTCAG
  CAGACGTCTCTGTTCCTGCTGAAACATGGCTTGACCAGAGATCCTATCCATCAGAGGGAGAGAGTCGATG
  TCTTCCCACAGTTTAACAAGCCGCCTTGGGTTTTTAGGATTAGTAAGCTGTCCCGCCTTATCGTGCCCAT
  CTTCACGCTGAATGAACAGCTCTGTTTTTCCAAGCTGCAGATACGGGACCGGCCTAGATTCGCAGGACGG
  GGGACCTACGGAAGAGTCCACATCTACCCATCATCCAAAATTGCCGTCAAAACCATGGACTCCCGGGTCT
  TTAATAGGGAATTGATCAACGCAATCCTGGCATCCGAAGGAAGCATCCGCGCCGGGGAACGACTGGGGAT
  CAGCTCCATCGTCTGTCTGCTCGGGTTCTCTCTCCAGACCAAACAGCTGCTTTTCCCTGCCTATGACATG
  GATATGGATGAGTATATCGTTAGACTGTCTCGACGGCTCACAATCCCTGATCATATCGATCGCAAAATCG
  CCCACGTGTTTCTTGATCTGGCACAGGCTTTGACTTTTTTGAACCGCACCTGTGGCCTCACACATCTCGA
  CGTGAAGTGCGGCAACATCTTTCTCAATGTTGACAACTTTGCTTCCTTGGAGATAACTACGGCGGTGATC
  GGCGACTACAGCCTGGTTACACTCAACACATATTCCCTGTGTACGCGGGCAATCTTCGAGGTGGGAAATC
  CCTCCCACCCTGAACATGTGTTGAGGGTACCACGAGATGCCAGTCAGATGTCCTTTAGGCTGGTGCTGAG
  CCACGGGACTAACCAACCCCCTGAGATCCTGCTGGACTATATTAATGGTACAGGACTGACAAAATACACC
  GGGACACTGCCGCAGAGAGTGGGCCTTGCTATCGATCTGTACGCGCTCGGACAGGCACTCCTGGAGGTTA
  TTCTGCTTGGGAGACTCCCTGGGCAGCTGCCTATCTCCGTGCACAGAACTCCACATTACCACTACTATGG
  CCACAAGCTTTCACCCGATCTCGCCTTGGATACCCTGGCCTACAGGTGTGTTCTGGCACCTTACATTCTC
  CCGTCAGACATTCCCGGAGATCTGAACTACAATCCATTCATACACGCCGGAGAGTTGAATACAAGGATTA
  GCAGGAATTCCCTGCGCAGAATTTTTCAGTGCCATGCTGTTCGGTACGGGGTCACCCACTCCAAATTGTT
  CGAAGGGATTCGGATCCCCGCTAGTCTCTATCCTGCCACAGTTGTGACTTCCCTGCTTTGCCACGACAAC
  AGCGAGATCAGATCTGACCATCCTCTGCTTTGGCACGACAGGGACTGGATTGGTTCTACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100988 representing NP_040169
  Red=Cloning sites Green=Tags
  
  MDADDTPPNLQISPTAGPLRSHHNTDGHEPNATAADQQERESTNPTHGCVNHPWANPSTATCMESPERSQ
  QTSLFLLKHGLTRDPIHQRERVDVFPQFNKPPWVFRISKLSRLIVPIFTLNEQLCFSKLQIRDRPRFAGR
  GTYGRVHIYPSSKIAVKTMDSRVFNRELINAILASEGSIRAGERLGISSIVCLLGFSLQTKQLLFPAYDM
  DMDEYIVRLSRRLTIPDHIDRKIAHVFLDLAQALTFLNRTCGLTHLDVKCGNIFLNVDNFASLEITTAVI
  GDYSLVTLNTYSLCTRAIFEVGNPSHPEHVLRVPRDASQMSFRLVLSHGTNQPPEILLDYINGTGLTKYT
  GTLPQRVGLAIDLYALGQALLEVILLGRLPGQLPISVHRTPHYHYYGHKLSPDLALDTLAYRCVLAPYIL
  PSDIPGDLNYNPFIHAGELNTRISRNSLRRIFQCHAVRYGVTHSKLFEGIRIPASLYPATVVTSLLCHDN
  SEIRSDHPLLWHDRDWIGST
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001348.1, NP_040169
• RefSeq ORF: 1530
• Locus ID: 1487678
• MW: 57.4 kDa