ORF66 (NC_001348) Virus Tagged ORF Clone

CAT#: VC101007

Myc-DDK-tagged ORF clone of viral ORF for serine/threonine protein kinase US3 [Human herpesvirus 3], codon optimized for human cell expression, NP_040188

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORF66
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101007 represents NCBI reference of NP_040188 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGATGTCGATGCCACAGACACGTTCGTCGGACAGGGTAAATTTAGGGGAGCCATAAGTACTTCCC
  CCTCACACATTATGCAGACCTGCGGTTTCATTCAGCAGATGTTTCCTGTGGAGATGTCCCCCGGAATTGA
  ATCTGAGGATGATCCGAACTACGACGTAAACATGGATATACAGTCATTTAACATATTCGACGGTGTTCAT
  GAGACCGAGGCCGAGGCTTCAGTGGCTCTCTGTGCTGAGGCTCGGGTCGGAATTAATAAAGCTGGCTTCG
  TGATCCTGAAAACTTTCACTCCCGGCGCTGAGGGATTTGCGTTCGCCTGCATGGACTCTAAAACCTGCGA
  GCACGTCGTGATCAAAGCGGGGCAGCGGCAGGGGACCGCAACAGAAGCAACCGTCCTTAGAGCCCTGACC
  CACCCAAGTGTTGTGCAGCTCAAGGGCACATTCACGTATAACAAAATGACTTGTCTTATCTTGCCTCGGT
  ACCGGACGGATCTTTACTGCTATCTTGCAGCCAAACGCAATCTTCCCATCTGTGATATCCTGGCTATCCA
  GCGCTCTGTGTTGCGCGCTCTGCAGTACCTGCACAACAATAGTATCATACATAGAGACATAAAGAGCGAG
  AACATCTTTATCAATCACCCTGGCGACGTGTGCGTGGGCGACTTTGGAGCGGCCTGTTTTCCAGTGGACA
  TCAATGCGAATCGGTATTACGGCTGGGCAGGCACTATCGCAACAAACTCCCCCGAGCTGCTTGCAAGAGA
  CCCCTACGGTCCTGCCGTTGATATATGGAGCGCCGGAATTGTACTCTTTGAGATGGCAACGGGACAGAAC
  TCTCTGTTTGAGAGGGACGGGCTGGACGGGAACTGCGACTCAGAGAGGCAAATCAAACTTATTATAAGAC
  GCTCCGGCACCCACCCCAACGAGTTCCCAATCAACCCCACAAGTAACCTGCGCCGACAGTACATCGGATT
  GGCAAAGAGATCCAGTAGGAAGCCAGGATCCAGGCCCCTGTGGACCAATCTGTACGAGCTCCCTATCGAT
  TTGGAATATCTTATCTGCAAAATGCTGTCCTTCGATGCCAGACACAGGCCCTCAGCTGAGGTTCTCCTGA
  ATCATTCAGTCTTCCAAACTTTGCCTGATCCATACCCCAACCCCATGGAGGTGGGTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101007 representing NP_040188
  Red=Cloning sites Green=Tags
  
  MNDVDATDTFVGQGKFRGAISTSPSHIMQTCGFIQQMFPVEMSPGIESEDDPNYDVNMDIQSFNIFDGVH
  ETEAEASVALCAEARVGINKAGFVILKTFTPGAEGFAFACMDSKTCEHVVIKAGQRQGTATEATVLRALT
  HPSVVQLKGTFTYNKMTCLILPRYRTDLYCYLAAKRNLPICDILAIQRSVLRALQYLHNNSIIHRDIKSE
  NIFINHPGDVCVGDFGAACFPVDINANRYYGWAGTIATNSPELLARDPYGPAVDIWSAGIVLFEMATGQN
  SLFERDGLDGNCDSERQIKLIIRRSGTHPNEFPINPTSNLRRQYIGLAKRSSRKPGSRPLWTNLYELPID
  LEYLICKMLSFDARHRPSAEVLLNHSVFQTLPDPYPNPMEVGD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001348
• ORF Size: 1179 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001348.1, NP_040188
• RefSeq ORF: 1179
• Locus ID: 1487703
• MW: 43.7 kDa