Secondary Antibodies

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Human IgE mouse monoclonal antibody, clone NI 307, FITC

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone BE5, FITC

Applications Flow cytometry: 5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), FITC

Applications To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques Immunofluorescence staining techinqiues.
General Recommended Dilutions:
Histochemical Use: from 1/10 upwards.
Reactivities Human
Conjugation FITC

Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), FITC

Applications To identify the presence of IgA1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA1, and Immunoblotting 
using a peroxidase labelled monoclonal antibody against FITC. 
General Recommended Dilutions
Histochemical Use: 1/20-1/100.  
Western blot: 1/200 upwards.
Reactivities Human
Conjugation FITC

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), FITC

Applications To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA2, and Immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100. 
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation FITC

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), FITC

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA2(m)2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation FITC

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), FITC

Applications To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation FITC

Human IgD mouse monoclonal antibody, clone NI 158, FITC

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgD and cell membrane IgD.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
Reactivities Human
Conjugation FITC

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), FITC

Applications To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG2 (F(ab)2 specific) mouse monoclonal antibody, clone NI 6014 (HP 6014), FITC

Applications To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), FITC

Applications To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50.
ELISA: from 1/200 upwards.
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), FITC

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect Immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards
Reactivities Human
Conjugation FITC

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), FITC

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. 
General Recommended Dilutions:
Histochemical Use: 1/10-1/50. 
ELISA: from 1/200 upwards. 
Western blot: from 1/400 upwards.
Reactivities Human
Conjugation FITC

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, FITC

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
ELISA: from 1/200 upwards
Western blot: from 1/400 upwards
Reactivities Human
Conjugation FITC

Human IgM mouse monoclonal antibody, clone NI 179, FITC

Applications To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
General Recommended Dilutions:
Histochemical Use: 1/10-1/50
Reactivities Human
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
  1 ≤ µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, FITC

Applications FLISA: 1/200-1/400
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation FITC

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, FITC

Applications Flow Cytometry: < / = 1.0 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Reactivities Rat
Conjugation FITC

Chicken IgG / Chicken IgY (gamma chain specific) mouse monoclonal antibody, clone G-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation FITC

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation FITC

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, FITC

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, FITC

Applications FLISA: 1/200-1/600.
Indirect Immunofluorescent staining
of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
Reactivities Human
Conjugation FITC

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, FITC

Applications FLISA:1:200-1:400
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgM mouse monoclonal antibody, clone ICL-931, FITC

Applications ELISA.
Immunohistochemistry on Frozen Sections: 1/40. 
Staining of Human cells and tissue sections.
Reactivities Human
Conjugation FITC

Human IgG mouse monoclonal antibody, clone EFE-565, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Human IgA mouse monoclonal antibody, clone BFG-267, FITC

Applications ELISA.
Immunohistochemistry on frozen sections: 1/40.
Reactivities Human
Conjugation FITC

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, FITC, Purified

Applications ELISA: 1/1000-1/10000. 
Immunofluorescence.
Reactivities Human
Conjugation FITC

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, FITC

Applications Flow Cytometry: Use 10 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-3, FITC

Applications Flow Cytometry: 5 µg/ml, use 50 µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Human IgE mouse monoclonal antibody, clone 4H10, FITC

Applications Flow cytometry: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Rabbit IgG mouse monoclonal antibody, clone 2A9, FITC

Applications Flow Cytometry: < / = 3 µg/10e6 cells.
Reactivities Rabbit
Conjugation FITC

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
Reactivities Chicken
Conjugation FITC

Human IgM (Mu chain specific) mouse monoclonal antibody, clone UHB, FITC

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, FITC

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, FITC

Applications Direct Immunofluorescence analysis: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, FITC

Applications Flow cytometry (5 µg/ml): use 50 µl of the suggested working dilution to label
10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgE mouse monoclonal antibody, clone MARE-1, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 2 x 10e7 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgG1 (heavy chain) mouse monoclonal antibody, clone MARG1.2, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, FITC

Applications Flow Cytometry: 10 µg/ml; use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG2c (heavy chain) mouse monoclonal antibody, clone MARG2c-5, FITC

Applications Flow cytometry: 5 µg/ml; use 10 µl of diluted antibody to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Human IgM (Fc-region) mouse monoclonal antibody, clone CH2, FITC

Applications Flow cytometry (1/500 starting dilution).
Reactivities Human
Conjugation FITC