Human IgG rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Immunodiffusion. For Ouchterlony: Sample Size: 15 µl Distance between wells: 5 mm Test time: 4 days at 37°C Antigens tested: Normal human serum, purified human IgG, normal mouse serum. Results: Titre with NHS > 1:1000. |
| Reactivities | Human |
Mouse IgG rabbit polyclonal antibody, Serum
| Reactivities | Mouse |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used: In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgM from serum or plasma. This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available. Recommended Working Dilutions: Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum. Double Radial Immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre: 1/32 when tested against pooled normal mouse serum in agar-block immunodiffusion titration. |
| Reactivities | Mouse |
| Immunogen | Highly purified homogenous IgM isolated from Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Equine IgM rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgM from serum or plasma. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/32 when tested against normal Horse serum in agar block titration. |
| Reactivities | Equine |
| Immunogen | Highly purified normal IgM isolated from pooled Horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Equine IgA rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgA from serum or plasma. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/32 when tested against normal Horse serum in agar block titration. |
| Reactivities | Equine |
| Immunogen | Highly purified normal IgA isolated from pooled Horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal sheep serum, to prepare an immunoadsorbent for the production of Ig-free sheep serum, plasma or non-Ig preparations. Recommended Working Dilutions: Immunoelectrophoresis: use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in sheep serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of sheep IgM from serum or plasma. Recommended Working Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Single Radial Immunodiffusion and electroimmunodiffusion: Use 0.5 to 1.0 percent antiserum in the agar gel. Antibody titre: 1/16 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Highly purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgM and IgG antibodies, as secondary antibody to precipitate the immunoglobulin in normal sheep serum, to prepare an immunoadsorbent for the production of Ig-free sheep serum, plasma or non-Ig preparations. Recommended Working Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Highly purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgA (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony and Mancini) to identify the presence of IgA in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or plasma. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available. Recommended Working Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: 1/32 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Immunogen | Highly purified normal IgA isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in swine serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgG from serum or plasma. The antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive techniques. For such uses labelled and unlabelled cytochemical grade reagents meeting the necessary additional specificity and performance requirements are available. Recommended Working Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Immunogen | Highly purified normal IgG isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Precipitation. Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal swine serum; to prepare an immunoadsorbent for the production of Ig-free swine serum, plasma or non-Ig preparations. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody Titre: Precipitin titre not less than 1/64 when tested against normal swine serum in agar block titration. |
| Reactivities | Porcine |
| Immunogen | Purified normal IgG isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgM (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Precipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgM from serum or plasma. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Single Radial Immunodiffusion and electroimmunodiffusion: Use 0.5 to 1.0 percent antiserum in the agar gel. Antibody Titre: Precipitin titre not less than 1/32 when tested against pooled normal swine serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Immunogen | Highly purified normal IgM isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilution(s): FLISA : 1:10,000 - 1:50,000 IF Microscopy : 1:1,000 - 1:5,000 Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500 |
| Conjugation | TRITC |
| Immunogen | Mouse IgG whole molecule. |
Canine IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This antibody has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:16,000 to 1:72,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Dog IgG whole molecule |
Goat IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000. |
| Conjugation | FITC |
| Immunogen | Goat IgG whole molecule |
Goat IgG (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy (1/1,000-1/5,000) and Flow Cytometry (1/500-1/2,500) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | TRITC |
| Immunogen | Goat IgG, whole molecule |
Hamster IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilution(s): Flow Cytometry: 1/2,000-1/10,000. IF Microscopy: 1/500-1/2,500. |
| Conjugation | FITC |
| Immunogen | Hamster IgG whole molecule. |
Equine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (1/1,000-1/10,000), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/200,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Horse IgG F(c) fragment. |
Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot: use at 1/1,000-1/5,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (use at 1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Horse IgG F(ab')2 fragment. |
Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/50,000-1/100,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:9,000 to 1:41,000 is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Human IgG whole molecule. |
Human IgG (F(ab)2 specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/25,000. Western Blot: 1/2,000-1/10,000. Immunohistochemisstry: 1/1,000-1/5,000. |
| Immunogen | Human IgG F(ab')2 fragment. |
Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, FITC
| Applications | Suitable for immunomicroscopy, Fluorescent based plate assays (FLISA) and Fluorescent Western Blotting. Also Suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy : 1/1,000-1/5,000. |
| Conjugation | FITC |
| Immunogen | Human IgG F(ab')2 fragment |
Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | Biotin |
| Immunogen | Mouse IgG whole molecule. |
Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, FITC
| Applications | This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Immunofluorescence: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500. |
| Conjugation | FITC |
| Immunogen | Mouse IgG2a heavy chain. |
Rat IgG F(ab)2 specific rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
| Immunogen | Rat IgG F(ab')2 fragment. |
Rat IgM (Mu chain specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Rat IgM mu heavy chain. |
Sheep IgG F(c) specific(adsorbed) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.. |
| Immunogen | Sheep IgG F(c) fragment. |
Porcine IgG F(ab)2 specific rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
| Immunogen | Swine IgG F(ab')2 fragment. |
Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. FLISA: 1:10,000/1:50,000 IF Microscopy: 1:1000/1:5000 |
| Conjugation | FITC |
| Immunogen | Rat IgG whole molecule. |
Sheep IgA rabbit polyclonal antibody, HRP
| Applications | ELISA: 1:10,000 - 1:100,000. |
| Reactivities | Sheep |
| Conjugation | HRP |
FITC Conjugated Rabbit Anti-Goat IgG
| Applications | FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64 |
| Reactivities | Goat IgG |
| Conjugation | FITC |
| Immunogen | Goat IgG, whole molecule |
FITC Conjugated Rabbit Anti-human IgG
| Applications | FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64 |
| Reactivities | Human IgG |
| Conjugation | FITC |
| Immunogen | Human IgG, whole molecule |
Mouse IgG2a rabbit polyclonal antibody, Purified
| Applications | ELISA. |
| Reactivities | Mouse |
| Immunogen | Mouse IgG2a paraproteins |
Human IgD (F(ab)2 Fragment) rabbit polyclonal antibody, Purified
| Applications | ELISA |
| Reactivities | Human |
| Immunogen | Human IgD |
Goat IgA rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. |
| Reactivities | Goat |
| Conjugation | HRP |
| Immunogen | Purified goat IgA. |
Goat IgM rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. |
| Reactivities | Goat |
| Conjugation | HRP |
| Immunogen | Purified Goat IgM |
Human IgD rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit immunoglobulin as a detection system. Positive Control: Lymph node. |
| Reactivities | Human |
| Immunogen | Purified IgD |
Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis, single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate immunoglobulins in normal monkey serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available. Recommmended dilutions: Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum. Double radial immunodiffusion: use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Monkey |
| Immunogen | Purified polyclonal IgG, IgA and IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal monkey serum; to prepare an immunoadsorbent for the production of Ig-free monkey serum, plasma or non-Ig preparations. Recommended dilutions: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Monkey |
| Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgD (Delta chain specific) rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on paraffin embedded sections: diluted of 1:40-1:100 in an ABC method (30-60 minutes at room temperature) Recommended Positive Control: Tonsil |
| Reactivities | Human |
| Immunogen | IgD isolated from a pool of normal human plasma. |
Human IgD (Delta chain specific) rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on paraffin embedded sections: diluted of 1:40-1:100 in an ABC method (30-60 minutes at room temperature) Recommended Positive Control: Tonsil |
| Reactivities | Human |
| Immunogen | IgD isolated from a pool of normal human plasma. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | AP |
| Immunogen | Donkey IgG whole molecule. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:45,000 to 1:150,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | Biotin |
| Immunogen | Donkey IgG whole molecules. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | FITC |
| Immunogen | Donkey IgG whole molecule. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Donkey IgG whole molecule. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
| Immunogen | Donkey IgG whole molecules. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | TRITC |
| Immunogen | Donkey IgG whole molecule. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | Texas Red |
| Immunogen | Donkey IgG whole molecule. |
Mouse IgG (H+L chain) rabbit polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring high titer binding and lot-to-lot consistency. Recommended Dilutions: IF Microscopy : 1:500 - 1:2,500 Other Dilution: User Optimized |
| Conjugation | Texas Red |
| Immunogen | Mouse IgG whole molecule. |
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