Secondary Antibodies

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Canine IgG2 rabbit polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Western Blot: 1:1000 - 1:10,000.
Immunohistochemistry on frozen sections: 1:200 - 1:500.
Reactivities Canine
Conjugation HRP

Peroxidase Conjugated Rabbit Anti-Rat IgG (gamma-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Rat IgG
Conjugation HRP
Immunogen Rat IgG, whole molecule

Rabbit Monoclonal Anti-Mouse IgM (mu-Chain) antibody, clone RM109

Applications ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL
Reactivities Mouse IgM mu-chain
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgA1 antibody, clone RM124

Applications ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgA1
Conjugation Unconjugated

Rabbit Monoclonal Anti-Human IgA2 antibody, clone RM125

Applications LISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human IgA2
Conjugation Unconjugated

Biotin Conjugated Rabbit Monoclonal Anti-Human Ig Light Chains antibody 3, clone RM129

Applications ELISA: 0.02ug/mL – 0.25ug/mL; IHC, ICC: 0.5ug/mL – 2ug/mL
Reactivities Human Ig light chain
Conjugation Biotin

Rabbit Monoclonal Anti-Human IgD Antibody, Clone RM123

Applications ELISA: 25ng/well - 200ng/well for Capture;, 0.01ug/mL - 0.1ug/mL for Detection;, , ICC: 0.5ug/mL-2ug/mL, IHC: 0.5ug/mL-2ug/mL
Reactivities Human IgD
Conjugation Unconjugated

Mouse IgA rabbit polyclonal antibody, Purified

Applications ELISA.
Reactivities Mouse, Rat
Immunogen Mouse IgA paraproteins

Goat IgM rabbit polyclonal antibody, Purified

Applications ELISA: 1/100 - 1/1000.
Immunodiffusion.
Reactivities Goat
Immunogen Purified goat IgM

Human IgM rabbit polyclonal antibody, Purified

Applications Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
Use Polymer anti Mouse/Rabbit immunoglobulin as a detection system.
Positive Control: Lymph node.
Reactivities Human
Immunogen Purified IgM.

Human IgA rabbit polyclonal antibody, Aff - Purified

Applications Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities Human

Rat IgE (Fc specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of homogenous IgE in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgE from serum or plasma.
Recommended dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Rat
Immunogen Pools of purified homogenous IgE isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgE (Fc specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of homogenous IgE in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgE from serum or plasma.
Recommended dilutions:
Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum.
Double radial immunodiffusion (Ouchterlony): A rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Mouse
Immunogen Pools of purified homogenous IgE isolated from pooled mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Bovine IgG2 (subclass specific) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG2 in Bovine serum and other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of bovine IgG2 from serum or plasma.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal Bovine serum in agar block titration.
Reactivities Bovine
Immunogen Pools of purified normal IgG2 isolated from pooled Bovine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Bovine IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications This antibody can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in Bovine serum and other body fluids or to determine its concentration.
Can also be used to prepare an immunoadsorbent for the purification of Bovine IgM from serum or plasma.
Recommneded Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/16 when tested against normal Bovine serum in agar block titration.
Reactivities Bovine
Immunogen Highly purified normal IgM isolated from pooled Bovine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Feline IgA (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgA in Cat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of cat IgA from serum or plasma.
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially) diluted in 2 mm diameter peripheral wells.
Antibody titre: 1/32 (Precipitin titre) when tested against pooled normal Cat serum in agar-block immunodiffusion titration.
Reactivities Feline
Immunogen Highly purified normal IgA isolated from pooled Cat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in monkey serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgM from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Directions for use:
In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (Fab specific) rabbit polyclonal antibody, Serum

Applications Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.
Recommended working dilutions:
Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Purified Fab of normal Rhesus monkey IgG. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgA from serum or plasma. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended Working Dilutions:
Immunoelectrophoresis: 2 μl serum or equivalent against 120 μl antiserum.
Double Radial Immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgA isolated from Rhesus Monkey serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgG (Fc specific) rabbit polyclonal antibody, Serum

Applications In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in monkey serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of monkey IgG from serum or plasma.
Recommended working dilutions:
Immunoelectrophoresis: 2 μl serum or equivalent against 120 μl antiserum.
Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Reactivities Monkey
Immunogen Highly purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgG rabbit polyclonal antibody, Serum

Applications Immunoprecipitation.
Immunodiffusion.

For Ouchterlony:
Sample Size: 15 µl
Distance between wells: 5 mm
Test time: 4 days at 37°C
Antigens tested: Normal human serum, purified human IgG, normal mouse serum.
Results: Titre with NHS > 1:1000.
Reactivities Human

Mouse IgG rabbit polyclonal antibody, Serum

Reactivities Mouse

Mouse IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications Immunoprecipitation.
Can be used: In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgM from serum or plasma.
This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended Working Dilutions:
Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum.
Double Radial Immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. 
Precipitin titre: 1/32 when tested against pooled normal mouse serum in agar-block immunodiffusion titration.
Reactivities Mouse
Immunogen Highly purified homogenous IgM isolated from Mouse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Equine IgM rabbit polyclonal antibody, Serum

Applications Immunoprecipitation.
Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgM from serum or plasma.
Directions for Use:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal Horse serum in agar block titration.
Reactivities Equine
Immunogen Highly purified normal IgM isolated from pooled Horse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Equine IgA rabbit polyclonal antibody, Serum

Applications Immunoprecipitation.
Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgA from serum or plasma.
Directions for Use:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: Precipitin titre not less than 1/32 when tested against normal Horse serum in agar block titration.
Reactivities Equine
Immunogen Highly purified normal IgA isolated from pooled Horse serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Sheep IgG (H+L chain) rabbit polyclonal antibody, Serum

Applications Immunoprecipitation. 
Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal sheep serum, to prepare an immunoadsorbent for the production of Ig-free sheep serum, plasma or non-Ig preparations.
Recommended Working Dilutions:
Immunoelectrophoresis: use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Immunogen Purified normal IgG isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Sheep IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications Immunoprecipitation. 
Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in sheep serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of sheep IgM from serum or plasma.
Recommended Working Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. 
Single Radial Immunodiffusion and electroimmunodiffusion: Use 0.5 to 1.0 percent antiserum in the agar gel.
Antibody titre: 1/16 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Immunogen Highly purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Sheep IgM (H+L chain) rabbit polyclonal antibody, Serum

Applications Immunoprecipitation. 
Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgM and IgG antibodies, as secondary antibody to precipitate the immunoglobulin in normal sheep serum, to prepare an immunoadsorbent for the production of Ig-free sheep serum, plasma or non-Ig preparations.
Recommended Working Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. 
Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Immunogen Highly purified normal IgM isolated from pooled sheep serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Porcine IgA (Fc specific) rabbit polyclonal antibody, Serum

Applications Immunoprecipitation. 
Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony and Mancini) to identify the presence of IgA in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or plasma. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended Working Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. 
Antibody titre: 1/32 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Porcine
Immunogen Highly purified normal IgA isolated from pooled Swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Porcine IgG (Fc specific) rabbit polyclonal antibody, Serum

Applications Immunoprecipitation. 
Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in swine serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgG from serum or plasma. The antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive techniques. For such uses labelled and unlabelled cytochemical grade reagents meeting the necessary additional specificity and performance requirements are available.
Recommended Working Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. 
Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Porcine
Immunogen Highly purified normal IgG isolated from pooled swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Porcine IgG (H+L chain) rabbit polyclonal antibody, Serum

Applications Precipitation.
Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal swine serum; to prepare an immunoadsorbent for the production of Ig-free swine serum, plasma or non-Ig preparations.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. 
Antibody Titre: Precipitin titre not less than 1/64 when tested against normal swine serum in agar block titration.
Reactivities Porcine
Immunogen Purified normal IgG isolated from pooled swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Porcine IgM (Fc specific) rabbit polyclonal antibody, Serum

Applications Precipitation.
Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgM from serum or plasma.
Recommended Dilutions:
Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. 
Single Radial Immunodiffusion and electroimmunodiffusion: Use 0.5 to 1.0 percent antiserum in the agar gel.
Antibody Titre: Precipitin titre not less than 1/32 when tested against pooled normal swine serum in agar-block immunodiffusion titration.
Reactivities Porcine
Immunogen Highly purified normal IgM isolated from pooled swine serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Mouse IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
FLISA : 1:10,000 - 1:50,000
IF Microscopy : 1:1,000 - 1:5,000
Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500
Conjugation TRITC
Immunogen Mouse IgG whole molecule.

Canine IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This antibody has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:16,000 to 1:72,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Dog IgG whole molecule

Goat IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Conjugation FITC
Immunogen Goat IgG whole molecule

Goat IgG (H+L chain) rabbit polyclonal antibody, TRITC

Applications Suitable for Immunomicroscopy (1/1,000-1/5,000) and Flow Cytometry (1/500-1/2,500) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation TRITC
Immunogen Goat IgG, whole molecule

Hamster IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilution(s):
Flow Cytometry: 1/2,000-1/10,000.
IF Microscopy: 1/500-1/2,500.
Conjugation FITC
Immunogen Hamster IgG whole molecule.

Equine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/1,000-1/10,000), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/200,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Horse IgG F(c) fragment.

Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot: use at 1/1,000-1/5,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (use at 1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 µg of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Horse IgG F(ab')2 fragment.

Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/50,000-1/100,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.

Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:9,000 to 1:41,000 is suggested for this product.
Conjugation HRP
Immunogen Human IgG whole molecule.

Human IgG (F(ab)2 specific) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/25,000.
Western Blot: 1/2,000-1/10,000.
Immunohistochemisstry: 1/1,000-1/5,000.
Immunogen Human IgG F(ab')2 fragment.

Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, FITC

Applications Suitable for immunomicroscopy,  Fluorescent based plate assays (FLISA) and  Fluorescent Western Blotting.
Also Suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. 
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
IF Microscopy : 1/1,000-1/5,000.
Conjugation FITC
Immunogen Human IgG F(ab')2 fragment

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
Conjugation Biotin
Immunogen Mouse IgG whole molecule.

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Conjugation FITC
Immunogen Mouse IgG2a heavy chain.

Rat IgG F(ab)2 specific rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Rat IgG F(ab')2 fragment.

Rat IgM (Mu chain specific) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/50,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Rat IgM mu heavy chain.

Sheep IgG F(c) specific(adsorbed) rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity..
Immunogen Sheep IgG F(c) fragment.

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Immunogen Swine IgG F(ab')2 fragment.

Rat IgG (H+L chain), F(ab)2 Fragment rabbit polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA:  1:10,000/1:50,000
IF Microscopy:  1:1000/1:5000
Conjugation FITC
Immunogen Rat IgG whole molecule.

Sheep IgA rabbit polyclonal antibody, HRP

Applications ELISA: 1:10,000 - 1:100,000.
Reactivities Sheep
Conjugation HRP