Mouse IgG1 (heavy chain) rat monoclonal antibody, clone LO-MG1-2, HRP
| Applications | ELISA: 500 ng/ml. This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with SM1491P as the capture reagent. |
| Reactivities | Mouse |
| Conjugation | HRP |
USD 190.00
2 Weeks
Human IgM (Mu chain, Fab-Region) mouse monoclonal antibody, clone MA2, Purified
| Applications | ELISA. |
| Reactivities | Human |
Peroxidase Conjugated Goat Anti-Mouse IgM (u-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Mouse IgM |
| Conjugation | HRP |
| Immunogen | Mouse IgM, whole molecule |
Biotin Conjugated Goat Anti-Mouse IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Mouse IgG |
| Conjugation | Biotin |
| Immunogen | Mouse IgG, whole molecule |
Peroxidase Conjugated Rabbit Anti-Goat IgG (gamma-chain specific)
| Applications | WB: 1:3,000-10,000; ELISA: 1:20,000-40,000 |
| Reactivities | Goat IgG |
| Conjugation | HRP |
| Immunogen | Goat IgG, whole molecule |
Rabbit Monoclonal Anti-Mouse IgG2a Kappa antibody, clone RM107
| Applications | ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (non-reduced): 0.1ug/mL-0.5ug/mL |
| Reactivities | Mouse IgG2a kappa |
| Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Mouse IgG2b (gamma-2b) antibody, clone RM108
| Applications | ELISA: 0.005ug/mL – 0.2ug/mL, ICC, IHC: 0.5ug/mL-2ug/mL, WB (stronger on non-reduced): 0.1ug/mL-0.5ug/mL |
| Reactivities | Mouse IgG2b |
| Conjugation | Unconjugated |
Rabbit Monoclonal Anti-Human Kappa Light Chain antibody, clone RM126
| Applications | ELISA: 0.05ug/mL-0.2ug/ml; ICC/IHC: 0.5ug/mL-2ug/mL |
| Reactivities | Human kappa light chain |
| Conjugation | Unconjugated |
Human IgM (Mu chain, Fc-region) mouse monoclonal antibody, clone 2A6
| Reactivities | Human |
Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 9B4, Purified
| Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1-10 µg. |
| Reactivities | Chicken |
Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), Azide Free
| Applications | To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200 ELISA: from 1/500 upwards Western blot: 1/3,000-1/50,000. |
| Reactivities | Human |
USD 550.00
2 Weeks
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Purified
| Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
USD 680.00
2 Weeks
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), TRITC
| Applications | To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. General Recommended Dilutions: Histochemical Use: 1/10-1/50. ELISA: from 1/200 upwards. Western blot: from 1/400 upwards. |
| Reactivities | Human |
| Conjugation | TRITC |
USD 550.00
2 Weeks
Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Purified
| Applications | To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG2, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
USD 680.00
2 Weeks
Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), HRP
| Applications | To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
USD 680.00
2 Weeks
Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), HRP
| Applications | To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
USD 550.00
2 Weeks
Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Purified
| Applications | To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG4, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
USD 680.00
2 Weeks
Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, HRP
| Applications | To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/500 ELISA: from 1/1000 upwards Western blot: from 1/2000 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgM mouse monoclonal antibody, clone NI 179, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/250 upwards. Western blot: from 1/500 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
USD 680.00
2 Weeks
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Biotin
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/20 upwards ELISA: form 1/25 upwards Western blot: from 1/50 upwards. |
| Reactivities | Human |
| Conjugation | Biotin |
USD 680.00
2 Weeks
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), FITC
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques Immunofluorescence staining techinqiues. General Recommended Dilutions: Histochemical Use: from 1/10 upwards. |
| Reactivities | Human |
| Conjugation | FITC |
USD 450.00
2 Weeks
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Ascites
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/50-1/200. ELISA: form 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
USD 525.00
2 Weeks
Human IgG (Fc specific) mouse monoclonal antibody, clone 21G/3D3, Purified
| Applications | ELISA. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgE goat polyclonal antibody, FITC
| Applications | Flow Cytometry. |
| Reactivities | Human, Monkey |
| Conjugation | FITC |
| Immunogen | Native human IgE |
Sheep/Goat IgG donkey polyclonal antibody, HRP
| Applications | ELISA: 1/12800-1/25600. Western Blot: 1/20000. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Frozen Sections: 1/50-1/100. |
| Reactivities | Goat, Sheep |
| Conjugation | HRP |
| Immunogen | Sheep IgG |
Chicken IgA (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. Can be used to prepare conjugates of choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Immunohistochemistry: 1/50-1/250. Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Antibody titre: Precipitin titre 1/64 when tested against pooled normal chicken serum in agar-block immunodiffusion titration. |
| Reactivities | Chicken |
| Immunogen | Purified normal IgA isolated from pooled Chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Chicken IgA (Fc specific) goat polyclonal antibody, Biotin
| Applications | Immunocytochemical and Immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates. To demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases. Identification of a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure. Non-isotopic assay methodology (e.g. ELISA) to measure IgA in chicken serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. Immunocytochemistry: 1/50-1/250. Immunohistochemistry: 1/50-1/250. |
| Reactivities | Chicken |
| Conjugation | Biotin |
| Immunogen | Purified normal IgA isolated from pooled Chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Chicken IgA (Fc specific) goat polyclonal antibody, FITC
| Applications | Immunocytochemical and Immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates. To demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases. Identification of a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Immunocytochemistry: 1/10-1/40. Immunohistochemistry: 1/10-1/40. |
| Reactivities | Chicken |
| Conjugation | FITC |
| Immunogen | Purified IgA isolated from Chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Chicken IgA (Fc specific) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in chicken serum or other body fluids. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Immunocytochemistry: 1/50-1/250. Immunohistochemistry: 1/50-1/250. |
| Reactivities | Chicken |
| Conjugation | HRP |
| Immunogen | Purified normal IgA isolated from pooled Chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Chicken IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in chicken serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: Histochemical and Cytochemical: 1/50 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/5000. |
| Reactivities | Chicken |
| Conjugation | Biotin |
| Immunogen | Purified IgM isolated from chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. Can be used to prepare conjugates of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/4,000. Immunohistochemistry: 1/50-1/250. Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Antibody titre: Precipitin titre 1/16 when tested against pooled normal Duck serum in agar-block immunodiffusion titration. |
| Reactivities | Duck |
| Immunogen | Purified IgM isolated from Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Suitable for use in Immunocytochemical and iImunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. Immunohistochemistry and Immunocytochemistry: 1/50-1/250. Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Duck |
| Conjugation | Biotin |
| Immunogen | Purified IgM isolated from Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | Suitable for use in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure. Recommended Dilutions: 1/10-1/40. Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Duck |
| Conjugation | FITC |
| Immunogen | Purified normal IgM isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, FITC
| Applications | Can be used to identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of guinea pig origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: 1/20 - 1/80. |
| Reactivities | Guinea Pig |
| Conjugation | FITC |
| Immunogen | Pools of highly purified homogenous IgG2 isolated from guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgG2 (subclass specific) goat polyclonal antibody, Serum
| Applications | Immunoprecipitation. Intended for use in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2 in guinea pig serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgG2 from serum or plasma. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Precipitin titre: 1/32 when tested against pooled normal Guinea Pig serum in agar-block Immunodiffusion titration. |
| Reactivities | Guinea Pig |
| Immunogen | Pools of purified normal IgG2 isolated from pooled Guinea Pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | In immunocytochemical and immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgM isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: 1/10 - 1/40. |
| Reactivities | Guinea Pig |
| Conjugation | FITC |
| Immunogen | Purified IgM isolated from guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgD (Fc specific) goat polyclonal antibody, FITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/10 - 1/40, depending on the method used. |
| Reactivities | Human |
| Conjugation | FITC |
| Immunogen | Pools of purified homogenous IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgD (Fc specific) goat polyclonal antibody, TRITC
| Applications | Can be used in direct Immunofluorescence staining of cytoplasmic IgD of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: 1/10 - 1/30. |
| Reactivities | Human |
| Conjugation | TRITC |
| Immunogen | Purified monoclonal IgD isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Germany
Japan
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