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Rabbit IgA goat polyclonal antibody, HRP
| Applications | ELISA: 1/10000-1/100000. |
| Reactivities | Rabbit |
| Conjugation | HRP |
| Immunogen | Purified rabbit IgA |
Products
Turkey IgG (H+L chain) goat polyclonal antibody, AP
| Applications | ELISA: 1/2,000-1/4,000. |
| Conjugation | AP |
Turkey IgG (H+L chain) goat polyclonal antibody, HRP
| Applications | ELISA: 1/4,000-1/8,000. |
| Conjugation | HRP |
Human IgD rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit immunoglobulin as a detection system. Positive Control: Lymph node. |
| Reactivities | Human |
| Immunogen | Purified IgD |
Rat IgG2ab (subclass specific) goat polyclonal antibody, Serum
| Applications | Immunoprecipitation. In precipitating techniques as Immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a and/or IgG2b in Rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of Rat IgG2a/IgG2b from serum or plasma. Recommended Working Dilutions: Immunoelectrophoresis: 2 µl or equivalent against 120 µl antiserum. Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less then 1/64 when tested against pooled normal Rat serum in agar block immunodiffusion titration. |
| Reactivities | Rat |
| Immunogen | Pools of purified homogenous IgG2a and IgG2b isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Serum
| Applications | Can be used in immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal monkey serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available. Directions for use: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Monkey |
| Immunogen | Purified normal IgG, IgA and IgM fractions isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (H+L chain) goat polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal monkey serum; to prepare an immunoadsorbent for the production of Ig-free monkey serum, plasma or non-Ig preparations. Directions for use: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Monkey |
| Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis, single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate immunoglobulins in normal monkey serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available. Recommmended dilutions: Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum. Double radial immunodiffusion: use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Monkey |
| Immunogen | Purified polyclonal IgG, IgA and IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal monkey serum; to prepare an immunoadsorbent for the production of Ig-free monkey serum, plasma or non-Ig preparations. Recommended dilutions: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Monkey |
| Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
PrecipHen® (Chicken IgY) goat polyclonal antibody, Agarose
| Applications | Immunoprecipitation. |
| Reactivities | Chicken |
| Conjugation | Agarose |
| Immunogen | Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Production: Goat anti-Chicken IgY antibodies were affinity purified from the sera over a Chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated Agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50mM HEPES buffer (pH 7.6) with 5mM EDTA (TE buffer). Sodium Azide was then added to a final concentration of 0.02% (w/v). |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC
| Applications | Immunocytochemistry (1/200). In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. |
| Reactivities | Chicken |
| Conjugation | FITC |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Human IgA + IgG + IgM (F(ab)2 Fragment) goat polyclonal antibody, Aff - Purified
| Applications | ELISA. |
| Reactivities | Human |
Mouse IgG3 goat polyclonal antibody, Purified
| Applications | ELISA. |
| Reactivities | Mouse |
| Immunogen | Mouse IgG3 paraproteins. |
Mouse IgG goat polyclonal antibody, FITC, Aff - Purified
| Applications | Immunofluorescence: 1/500-1/1000. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | FITC |
| Immunogen | Purified Mouse IgG fraction |
Human IgA mouse monoclonal antibody, clone DA43D3, Purified
| Applications | Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended. Suggested Positive Control: Human tonsil. |
| Reactivities | Human |
Human IgM mouse monoclonal antibody, clone DM3A3, Purified
| Applications | Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400). Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended. Suggested positive control: Human tonsil. |
| Reactivities | Human |
Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, Purified
| Applications | ELISA: 1/500-1/1000. |
| Reactivities | Human |
Human IgA1 (Fc specific) mouse monoclonal antibody, clone 3C10, Purified
| Applications | ELISA. |
| Reactivities | Human |
Human IgA2 (Fc specific) mouse monoclonal antibody, clone 1A4, Purified
| Applications | ELISA. |
| Reactivities | Human |
Human IgD (Fc specific) mouse monoclonal antibody, clone LE2, Ig Fraction
| Applications | Flow cytometry. Immunohistochemistry on frozen tissue sections. |
| Reactivities | Human |
Human IgM mouse monoclonal antibody, clone 47.2.E9, Purified
| Applications | ELISA (1/5000-1/10000) |
| Reactivities | Human |
Human IgD (Delta chain specific) rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on paraffin embedded sections: diluted of 1:40-1:100 in an ABC method (30-60 minutes at room temperature) Recommended Positive Control: Tonsil |
| Reactivities | Human |
| Immunogen | IgD isolated from a pool of normal human plasma. |
Human IgD (Delta chain specific) rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on paraffin embedded sections: diluted of 1:40-1:100 in an ABC method (30-60 minutes at room temperature) Recommended Positive Control: Tonsil |
| Reactivities | Human |
| Immunogen | IgD isolated from a pool of normal human plasma. |
Monkey IgA + IgG + IgM goat polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/500-1/4,000. Western Blot: 1/500-1/2,500. Immunohistochemistry: 1/200-1/1,000. |
| Conjugation | AP |
| Immunogen | Monkey IgG, IgA and IgM whole molecule. |
Monkey IgA + IgG + IgM goat polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | FITC |
| Immunogen | Monkey IgG, IgA, IgM whole molecules. |
Monkey IgA + IgG + IgM goat polyclonal antibody, Aff - Purified
| Applications | Immunoprecipitation. Immunodiffusion. ELISA: 1/100000. Western Blot: 1/2000 - 1/10000. |
| Immunogen | Monkey IgG, IgA and IgM whole molecules |
Monkey IgA + IgG + IgM goat polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | TRITC |
| Immunogen | Monkey IgG, IgA and IgM whole molecule. |
Monkey IgA + IgG + IgM goat polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | Texas Red |
| Immunogen | Monkey IgG, IgA and IgM whole molecule. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Donkey IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | AP |
| Immunogen | Donkey IgG whole molecule. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:45,000 to 1:150,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | Biotin |
| Immunogen | Donkey IgG whole molecules. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | FITC |
| Immunogen | Donkey IgG whole molecule. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/100,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Donkey IgG whole molecule. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. |
| Immunogen | Donkey IgG whole molecules. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | TRITC |
| Immunogen | Donkey IgG whole molecule. |
Donkey IgG (H+L chain) rabbit polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | Texas Red |
| Immunogen | Donkey IgG whole molecule. |
Mouse IgG (H+L chain) rabbit polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring high titer binding and lot-to-lot consistency. Recommended Dilutions: IF Microscopy : 1:500 - 1:2,500 Other Dilution: User Optimized |
| Conjugation | Texas Red |
| Immunogen | Mouse IgG whole molecule. |
Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions: ELISA: 1/3,000-1/12,000. Western Blot: 1/500-1/2,000. Immunohistochemistry: 1/200-1/1,000. |
| Conjugation | AP |
| Immunogen | Goat IgG whole molecule. |
Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using Peroxidase conjugated strepavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | Biotin |
| Immunogen | Goat IgG whole molecule. |
Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/130,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Goat IgG whole molecule |
Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, Aff - Purified
| Immunogen | Goat IgG whole molecule Application(s): Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): Not specified. Optimal titers for applications should be determined by the researcher. |
Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | TRITC |
| Immunogen | Goat IgG whole molecule. |
Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | Texas Red |
| Immunogen | Goat IgG whole molecule. |
Rabbit IgG (H+L chain) sheep polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/15,000. Western Blot: 1/500-1/2,000. Immunohistochemistry: 1/200-1/1,000. |
| Conjugation | AP |
| Immunogen | Rabbit IgG whole molecule. |
Rabbit IgG (H+L chain) sheep polyclonal antibody, Biotin
| Applications | Immunoblotting, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. ELISA: 1:300.000. Western blot and Immunohistochemistry: user optimized. |
| Conjugation | Biotin |
| Immunogen | Rabbit IgG whole molecule |
Rabbit IgG (H+L chain) sheep polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | FITC |
| Immunogen | Rabbit IgG whole molecule. |
Rabbit IgG (H+L chain) sheep polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/40,000-1/200,000. Western blot: 1/4,000-1/20,000. Immunohistochemistry: 1/2,000-1/10,000. |
| Immunogen | Rabbit IgG whole molecule. |
Rabbit IgG (H+L chain) sheep polyclonal antibody, Texas Red
| Applications | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended dilutions: Flow cytometry 1:2,000 - 1:10,000 IF microscopy 1:500 - 1:2,500 |
| Conjugation | Texas Red |
| Immunogen | Rabbit IgG whole molecule |
Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Chicken IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product, in Western Blot 1:1,000, Immunohistochemistry 1:500. |
| Conjugation | AP |
| Immunogen | Chicken IgG whole molecule. |
Chicken IgG / Chicken IgY (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | TRITC |
| Immunogen | Chicken IgG whole molecule |
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