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Elane (Myc-DDK-tagged) - Mouse elastase, neutrophil expressed (Elane)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

ELANE (GFP-tagged) - Human elastase, neutrophil expressed (ELANE)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

Elane - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.

Format 2 gRNA vectors, 1 linear donor
Donor DNA EF1a-GFP-P2A-Puro
KN505141 is the updated version of KN305141.

Elane (GFP-tagged) - Mouse elastase neutrophil expressed (Elane), (10ug)

Vector pCMV6-AC-GFP
Tag TurboGFP
Mammalian Cell Selection Neomycin
  • TrueORF®

Lenti ORF clone of Elane (Myc-DDK-tagged) - Mouse elastase, neutrophil expressed (Elane)

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Elane (Myc-DDK-tagged) - Mouse elastase, neutrophil expressed (Elane), 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF clone of Elane (mGFP-tagged) - Mouse elastase, neutrophil expressed (Elane)

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, Elane (GFP-tagged) - Mouse elastase, neutrophil expressed (Elane), 200ul, >10^7 TU/mL

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF particles, ELANE (Myc-DDK tagged) - Human elastase, neutrophil expressed (ELANE), 200ul, >10^7 TU/mL

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Elane (Myc-DDK-tagged ORF) - Rat elastase 2, neutrophil (Ela2), (10 ug)

Vector pCMV6-Entry
Tag Myc-DDK
Mammalian Cell Selection Neomycin
  • TrueORF®

Lenti ORF clone of Elane (Myc-DDK-tagged ORF) - Rat elastase 2, neutrophil (Ela2), (10 ug)

Vector pLenti-C-Myc-DDK-P2A-Puro
Tag Myc-DDK
Mammalian Cell Selection Puromycin
  • LentiORF®

Lenti ORF clone of Elane (mGFP-tagged ORF) - Rat elastase 2, neutrophil (Ela2), (10 ug)

Vector pLenti-C-mGFP-P2A-Puro
Tag mGFP
Mammalian Cell Selection Puromycin
  • LentiORF®

ELANE (untagged)-Human elastase, neutrophil expressed (ELANE)

Vector pCMV6-XL4
Tag Tag Free
Mammalian Cell Selection None

Transient overexpression lysate of elastase, neutrophil expressed (ELANE)

Tag C-Myc/DDK
Expression Host HEK293T
Applications WB

Neutrophil Elastase sheep polyclonal antibody, Ion exchange chromatography

Applications ELISA, Ie, IHC
Reactivities Human
Conjugation Unconjugated
Immunogen Neutrophil elastase (active-site blocked) prepared from human sputum.
AP23184PU-N is a possible alternative to AP00714PU-N.

ELANE rabbit polyclonal antibody

Applications IHC
Reactivities Human
Conjugation Unconjugated
Immunogen Synthetic peptide of human ELANE

Purified recombinant protein ofHuman elastase, neutrophil expressed (ELANE), full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug

Tag N-GST and C-His
Expression Host E. coli

Elane (untagged ORF) - Rat elastase 2, neutrophil (Ela2), (10 ug)

Vector pCMV6-Entry
Tag Tag Free
Mammalian Cell Selection Neomycin

Elane (untagged) - Mouse elastase, neutrophil expressed (Elane), (10ug)

Vector pCMV6-Entry
Tag Tag Free
Mammalian Cell Selection Neomycin

Lenti ORF clone of Human elastase, neutrophil expressed (ELANE), mGFP tagged

Vector pLenti-C-mGFP
Tag mGFP
Mammalian Cell Selection None
  • LentiORF®

ELANE - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector

Format Retroviral plasmids
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin

qSTAR qPCR primer pairs against Homo sapiens gene ELANE

Component 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions)

ELANE HEK293T cell transient overexpression lysate (as WB positive control)

Tag C-Myc/DDK
Expression Host HEK293T
Applications WB

Neutrophil Elastase (ELANE) (N-term) rabbit polyclonal antibody, Aff - Purified

Applications WB
Reactivities Human
Immunogen Synthetic peptide - KLH conjugated -  between 13-39 amino acids from the N-terminal region (between 20-47aa ) of human Neutrophil elastase

qSTAR qPCR primer pairs against Mus musculus gene Elane

ELANE - Human shRNA lentiviral particles (4 unique 29mer target-specific shRNA, 1 scramble control), 0.5 ml each, >10^7 TU/ml.

Format Lentiviral particles
Vector pGFP-C-shLenti

Neutrophil elastase human protein, 0.1 mg

Protein Source Neutrophils

For quantitative detection of mouse Elastase in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

Assay Type Sandwich ELISA kit of Quantitative Detection for mouse Elastase
Format 8x12 divisible strips
Reactivities Mouse

For quantitative detection of human Elastase/ELANE/ELA2 in cell culture supernates, serum and plasma (heparin, EDTA).

Assay Type Sandwich ELISA kit of Quantitative Detection for Human Elastase/ELANE/ELA2
Format 8x12 divisible strips
Reactivities Human

ELANE CRISPRa kit - CRISPR gene activation of human elastase, neutrophil expressed

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

Elane CRISPRa kit - CRISPR gene activation of mouse elastase, neutrophil expressed

Format 3gRNAs, 1 scramble ctrl and 1 enhancer vector

qPCR primer pairs and template standards against Homo sapiens gene ELA2

Application Plasmid of exact quantity for transcript copy number calculation

3`UTR clone of elastase neutrophil expressed (ELANE) for miRNA target validation

Vector pMirTarget
Mammalian Cell Selection Neomycin
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

ELANE (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Special offer: Get $100/€100 off this product. Use code: SR100

SR320022 is the updated version of SR301379.

Elane (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

Special offer: Get $100/€100 off this product. Use code: SR100

ELANE rabbit polyclonal antibody

Applications IHC
Reactivities Human
Conjugation Unconjugated
Immunogen Synthetic peptide of human ELANE

Neutrophil Elastase (ELANE) Rabbit polyclonal Antibody

Applications IF, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 30-267 of human Neutrophil Elastase (ELANE) (NP_001963.1).
Modifications Unmodified

Transient overexpression of ELANE (NM_001972) in HEK293T cells paraffin embedded controls for ICC/IHC staining

ELANE - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector

Format Lentiviral plasmids
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin