tat (NC_001802) Virus Tagged ORF Clone

CAT#: VC101716

Myc-DDK-tagged ORF clone of viral ORF for Tat [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057853

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: tat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101716 represents NCBI reference of NP_057853 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCCGTTGACCCTCGACTGGAGCCATGGAAACATCCTGGATCACAACCAAAAACAGCCTGCACTA
  ACTGCTACTGCAAAAAGTGCTGCTTCCATTGTCAAGTGTGCTTTATCACAAAGGCACTGGGTATCAGTTA
  TGGCAGAAAGAAGCGCCGGCAGCGGCGACGAGCCCACCAGAATAGCCAGACCCACCAAGCCAGCCTGTCA
  AAGCAACCCACATCCCAACCTCGCGGCGACCCGACAGGACCTAAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101716 representing NP_057853
  Red=Cloning sites Green=Tags
  
  MEPVDPRLEPWKHPGSQPKTACTNCYCKKCCFHCQVCFITKALGISYGRKKRRQRRRAHQNSQTHQASLS
  KQPTSQPRGDPTGPKE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 258 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001802.1, NP_057853
• RefSeq ORF: 258
• Locus ID: 155871
• MW: 9.8 kDa