vpu (NC_001802) Virus Tagged ORF Clone

CAT#: VC101723

Myc-DDK-tagged ORF clone of viral ORF for Vpu [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057855

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: vpu
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101723 represents NCBI reference of NP_057855 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCTATCCCAATAGTGGCTATTGTGGCACTGGTGGTGGCCATCATCATAGCCATCGTCGTGTGGA
  GTATTGTTATAATCGAGTATCGCAAGATTCTGCGACAGCGGAAGATAGATCGGCTGATCGATCGGTTGAT
  TGAGCGCGCCGAAGATTCCGGCAATGAATCCGAAGGGGAAATTTCAGCCCTGGTCGAGATGGGAGTCGAA
  ATGGGGCATCATGCCCCCTGGGATGTCGACGACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101723 representing NP_057855
  Red=Cloning sites Green=Tags
  
  MQPIPIVAIVALVVAIIIAIVVWSIVIIEYRKILRQRKIDRLIDRLIERAEDSGNESEGEISALVEMGVE
  MGHHAPWDVDDL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 246 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001802.1, NP_057855
• RefSeq ORF: 246
• Locus ID: 155945
• MW: 9.2 kDa