gag (NC_001802) Virus Tagged ORF Clone

CAT#: VC101718

Myc-DDK-tagged ORF clone of viral ORF for Pr55(Gag) [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057850

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: gag
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101718 represents NCBI reference of NP_057850 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCTAGAGCCTCTGTGTTGAGTGGCGGGGAGTTGGATCGGTGGGAGAAGATTCGACTGCGGCCTG
  GGGGTAAAAAGAAGTACAAGCTGAAGCACATCGTGTGGGCTTCAAGGGAGCTTGAGCGCTTCGCGGTCAA
  TCCCGGACTGCTGGAGACGAGCGAAGGATGCCGCCAGATCCTCGGACAGCTGCAGCCCTCCCTGCAGACA
  GGTTCTGAGGAGCTCAGGAGCCTGTACAACACAGTGGCAACCCTGTATTGCGTTCACCAGAGAATCGAGA
  TCAAAGATACCAAGGAGGCCCTCGATAAGATCGAGGAGGAGCAAAACAAGTCTAAGAAGAAGGCTCAACA
  GGCCGCCGCGGACACTGGGCACAGCAACCAGGTTTCCCAAAATTATCCCATCGTGCAGAACATCCAGGGG
  CAGATGGTCCACCAGGCTATCTCCCCGCGAACCCTTAATGCCTGGGTTAAAGTTGTGGAGGAAAAGGCTT
  TTTCCCCTGAAGTGATACCTATGTTCTCCGCCTTGAGTGAAGGCGCCACTCCGCAGGACCTCAATACCAT
  GCTCAATACAGTGGGCGGGCATCAGGCCGCTATGCAGATGCTTAAAGAAACCATCAACGAGGAGGCAGCA
  GAATGGGACCGAGTGCACCCAGTGCACGCTGGTCCAATAGCACCTGGACAAATGCGGGAACCCCGGGGCT
  CAGATATTGCCGGCACGACATCAACCCTCCAAGAGCAGATCGGATGGATGACTAATAACCCCCCTATTCC
  CGTGGGAGAAATATATAAGAGATGGATAATCCTGGGCCTGAACAAAATAGTCAGAATGTACTCACCTACT
  AGTATCCTGGACATTAGGCAAGGACCAAAGGAGCCCTTCAGAGATTACGTAGACAGATTCTATAAGACCC
  TTCGGGCTGAACAGGCTTCCCAGGAGGTCAAGAATTGGATGACCGAGACCCTTCTCGTGCAAAACGCAAA
  TCCGGACTGTAAAACCATTCTGAAGGCTCTCGGTCCCGCAGCTACCCTCGAAGAGATGATGACAGCCTGC
  CAGGGCGTCGGTGGTCCGGGACACAAGGCCCGGGTTCTCGCTGAGGCCATGAGCCAGGTCACTAATAGCG
  CCACCATCATGATGCAGCGGGGTAACTTCCGCAACCAGAGGAAAATCGTCAAATGTTTCAACTGTGGAAA
  GGAAGGCCACACAGCGCGGAACTGCCGCGCTCCAAGGAAGAAAGGCTGCTGGAAGTGCGGCAAGGAGGGG
  CATCAGATGAAAGACTGCACAGAACGGCAGGCTAATTTCCTGGGAAAGATCTGGCCATCTTATAAGGGAA
  GACCCGGCAACTTCCTTCAATCAAGACCTGAACCGACCGCACCACCCGAAGAGAGCTTCAGAAGTGGCGT
  AGAAACCACCACTCCTCCTCAGAAGCAGGAACCAATTGACAAAGAGCTCTATCCTTTGACAAGCTTGCGC
  TCTCTGTTCGGAAATGATCCAAGCAGTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101718 representing NP_057850
  Red=Cloning sites Green=Tags
  
  MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFAVNPGLLETSEGCRQILGQLQPSLQT
  GSEELRSLYNTVATLYCVHQRIEIKDTKEALDKIEEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQG
  QMVHQAISPRTLNAWVKVVEEKAFSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAA
  EWDRVHPVHAGPIAPGQMREPRGSDIAGTTSTLQEQIGWMTNNPPIPVGEIYKRWIILGLNKIVRMYSPT
  SILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPAATLEEMMTAC
  QGVGGPGHKARVLAEAMSQVTNSATIMMQRGNFRNQRKIVKCFNCGKEGHTARNCRAPRKKGCWKCGKEG
  HQMKDCTERQANFLGKIWPSYKGRPGNFLQSRPEPTAPPEESFRSGVETTTPPQKQEPIDKELYPLTSLR
  SLFGNDPSSQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001802.1, NP_057850
• RefSeq ORF: 1500
• Locus ID: 155030
• MW: 55.9 kDa