Envelope surface glycoprotein gp120 (NC_001802) Virus Tagged ORF Clone

CAT#: VC101739

Myc-DDK-tagged ORF clone of viral ORF for Envelope surface glycoprotein gp120 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579894

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Envelope surface glycoprotein gp120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101739 represents NCBI reference of NP_579894 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCTACCGAGAAGCTGTGGGTTACTGTCTACTACGGCGTCCCTGTGTGGAAGGAAGCGACTACTA
  CACTGTTCTGTGCCTCTGATGCGAAGGCCTACGATACCGAAGTGCACAATGTTTGGGCTACACATGCCTG
  TGTTCCTACAGACCCCAATCCTCAGGAGGTGGTGCTCGTCAATGTTACCGAAAATTTTAACATGTGGAAG
  AACGACATGGTGGAGCAGATGCACGAAGATATCATTTCTCTCTGGGATCAATCTCTGAAGCCGTGTGTGA
  AACTTACACCACTGTGTGTAAGTCTGAAGTGTACTGACTTGAAGAACGACACCAACACCAACTCCTCCTC
  CGGTCGCATGATTATGGAAAAGGGCGAGATCAAAAACTGCAGCTTTAATATCAGCACAAGCATTAGGGGG
  AAAGTGCAGAAGGAGTATGCGTTCTTCTATAAGCTTGATATTATTCCAATCGATAACGACACCACCTCAT
  ACAAACTCACTAGCTGCAACACCAGCGTGATTACACAGGCTTGCCCTAAGGTTAGTTTCGAGCCCATTCC
  TATTCACTATTGCGCCCCCGCCGGATTTGCAATCCTTAAATGCAACAATAAGACTTTTAACGGGACCGGT
  CCATGCACAAATGTTAGCACTGTTCAATGTACTCACGGTATTCGCCCTGTGGTCAGTACCCAGCTGTTGC
  TCAACGGATCACTCGCAGAAGAGGAAGTCGTCATCAGAAGCGTAAACTTCACCGATAATGCTAAAACAAT
  CATTGTACAGCTGAACACAAGCGTGGAAATCAATTGCACCAGGCCCAATAACAACACAAGGAAGCGCATT
  CGCATTCAGAGAGGACCTGGAAGAGCATTCGTGACTATCGGAAAAATCGGCAATATGAGACAAGCGCACT
  GCAATATATCTCGGGCCAAGTGGAATAACACGCTGAAGCAGATCGCCTCAAAGCTGAGGGAACAATTCGG
  TAACAATAAGACGATTATTTTTAAGCAGTCCTCCGGAGGCGATCCAGAGATCGTGACCCACTCCTTCAAC
  TGTGGTGGGGAATTCTTTTATTGTAATAGTACACAGCTCTTCAATTCCACTTGGTTCAACAGCACATGGA
  GTACAGAAGGGTCCAACAATACCGAGGGCAGTGACACGATCACACTGCCTTGCAGAATCAAACAGATAAT
  AAATATGTGGCAAAAGGTTGGCAAAGCAATGTATGCTCCCCCAATAAGCGGCCAGATCAGGTGTTCAAGT
  AATATTACAGGGTTGTTGCTCACACGCGATGGCGGCAACTCAAACAATGAAAGTGAGATTTTTAGACCAG
  GCGGGGGCGACATGAGAGATAATTGGCGCAGTGAACTGTACAAATACAAGGTGGTGAAAATCGAACCCCT
  TGGTGTCGCTCCAACCAAGGCCAAGAGGCGGGTGGTGCAGCGGGAGAAAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101739 representing NP_579894
  Red=Cloning sites Green=Tags
  
  MSATEKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWK
  NDMVEQMHEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNTNSSSGRMIMEKGEIKNCSFNISTSIRG
  KVQKEYAFFYKLDIIPIDNDTTSYKLTSCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGTG
  PCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSVNFTDNAKTIIVQLNTSVEINCTRPNNNTRKRI
  RIQRGPGRAFVTIGKIGNMRQAHCNISRAKWNNTLKQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFN
  CGGEFFYCNSTQLFNSTWFNSTWSTEGSNNTEGSDTITLPCRIKQIINMWQKVGKAMYAPPISGQIRCSS
  NITGLLLTRDGGNSNNESEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001802.1, NP_579894
• RefSeq ORF: 1452
• MW: 54.2 kDa