nef (NC_001802) Virus Tagged ORF Clone

CAT#: VC101722

Myc-DDK-tagged ORF clone of viral ORF for Nef [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057857

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nef
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101722 represents NCBI reference of NP_057857 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGGCAAGTGGTCCAAATCTTCTGTGATCGGATGGCCCACTGTGCGAGAGCGGATGCGACGGGCCG
  AACCTGCAGCGGACAGAGTGGGCGCGGCCAGCCGCGACCTGGAGAAACATGGCGCAATCACCTCCAGCAA
  CACAGCAGCCACGAACGCAGCCTGTGCCTGGCTTGAGGCTCAAGAAGAGGAGGAGGTGGGGTTTCCCGTC
  ACCCCGCAAGTACCTTTGAGGCCCATGACCTATAAGGCCGCCGTGGATCTGAGCCATTTCCTGAAAGAGA
  AAGGTGGATTGGAGGGGTTGATCCATAGTCAAAGGAGACAGGACATCCTGGACTTGTGGATTTATCATAC
  GCAGGGCTATTTTCCCGATTGGCAGAACTATACTCCTGGACCGGGCGTGCGCTACCCTCTGACTTTTGGC
  TGGTGCTACAAGCTGGTGCCAGTCGAGCCCGACAAAATTGAAGAGGCAAATAAGGGCGAGAACACCTCAC
  TTCTGCACCCTGTGTCCCTCCACGGCATGGATGACCCTGAGCGAGAAGTCCTGGAGTGGCGGTTTGATAG
  TCGGTTGGCCTTCCATCATGTAGCCAGGGAGCTGCACCCAGAATATTTCAAGAATTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101722 representing NP_057857
  Red=Cloning sites Green=Tags
  
  MGGKWSKSSVIGWPTVRERMRRAEPAADRVGAASRDLEKHGAITSSNTAATNAACAWLEAQEEEEVGFPV
  TPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFG
  WCYKLVPVEPDKIEEANKGENTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 618 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001802.1, NP_057857
• RefSeq ORF: 618
• Locus ID: 156110
• MW: 23.5 kDa