env (NC_001802) Virus Tagged ORF Clone

CAT#: VC101721

Myc-DDK-tagged ORF clone of viral ORF for Envelope surface glycoprotein gp160, precursor [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057856

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: env
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101721 represents NCBI reference of NP_057856 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGTTAAGGAAAAATATCAGCACCTCTGGAGGTGGGGATGGAGATGGGGCACTATGCTTCTCGGGA
  TGCTCATGATTTGCAGCGCAACCGAAAAGCTGTGGGTGACGGTATACTACGGCGTTCCAGTCTGGAAAGA
  GGCAACTACAACCCTTTTTTGCGCTTCCGACGCCAAGGCATATGACACTGAAGTCCATAACGTGTGGGCA
  ACCCACGCCTGTGTCCCAACGGATCCAAATCCCCAGGAAGTTGTATTGGTTAATGTGACTGAGAACTTTA
  ACATGTGGAAAAACGACATGGTCGAACAAATGCATGAAGATATTATTTCACTGTGGGATCAATCTCTGAA
  ACCTTGCGTCAAGCTGACACCTCTCTGTGTCTCACTTAAATGTACCGATCTGAAGAACGACACTAACACT
  AATTCTTCTAGCGGTAGAATGATCATGGAAAAGGGCGAGATCAAGAACTGCTCCTTTAATATTAGTACCA
  GTATTCGGGGTAAGGTCCAAAAGGAGTACGCTTTCTTTTACAAGCTCGATATTATTCCCATTGATAACGA
  TACCACCAGTTATAAACTGACTTCTTGTAACACGAGCGTGATCACTCAGGCTTGTCCCAAGGTTAGCTTC
  GAGCCAATTCCCATTCACTACTGCGCCCCTGCTGGTTTCGCCATCCTGAAGTGTAATAATAAAACATTTA
  ACGGAACCGGTCCGTGCACCAACGTGTCAACCGTCCAGTGTACGCATGGCATCCGACCTGTGGTTTCAAC
  TCAATTGTTGCTGAACGGCTCCTTGGCCGAGGAGGAGGTAGTCATCAGAAGCGTGAACTTCACCGATAAT
  GCCAAGACAATTATAGTGCAGCTGAATACAAGTGTTGAGATCAATTGTACCAGACCAAACAATAACACCC
  GCAAACGCATCCGCATCCAGAGAGGTCCTGGCCGGGCATTTGTGACGATCGGAAAGATCGGAAATATGCG
  ACAAGCCCACTGCAACATCTCCCGCGCGAAATGGAACAACACTCTCAAACAGATTGCCTCCAAACTCCGA
  GAGCAATTCGGGAACAACAAGACAATTATTTTCAAACAGAGTTCTGGCGGCGATCCAGAAATAGTGACTC
  ACTCATTTAATTGTGGCGGGGAGTTTTTCTACTGCAACAGCACCCAGCTCTTCAACTCTACCTGGTTTAA
  CAGCACTTGGTCCACAGAAGGCAGTAATAATACAGAGGGCTCAGACACAATTACACTGCCTTGCAGAATT
  AAACAAATAATAAATATGTGGCAGAAGGTCGGTAAGGCAATGTATGCTCCGCCTATCTCTGGCCAAATTA
  GATGTAGTTCAAACATAACCGGGCTTCTCTTGACCCGGGATGGTGGTAATTCTAATAACGAGAGCGAAAT
  CTTCAGACCCGGGGGCGGGGACATGCGGGACAACTGGCGGAGCGAGCTCTATAAATATAAGGTGGTGAAG
  ATCGAACCCTTGGGAGTCGCCCCAACGAAAGCTAAACGGAGAGTAGTCCAACGCGAGAAGCGGGCTGTAG
  GAATAGGCGCCCTGTTTCTTGGGTTCCTCGGGGCCGCCGGCTCAACTATGGGTGCCGCATCCATGACACT
  CACCGTGCAGGCACGCCAATTGCTGTCAGGGATCGTTCAGCAGCAGAATAACCTCCTGCGCGCGATAGAG
  GCTCAGCAGCATCTGCTGCAGCTGACCGTATGGGGCATCAAGCAGTTGCAGGCCCGCATCTTGGCGGTTG
  AGCGGTACCTTAAAGATCAGCAGCTGCTCGGGATCTGGGGATGCAGTGGGAAGTTGATCTGTACAACAGC
  AGTGCCCTGGAACGCTTCTTGGTCAAACAAGTCTCTGGAACAAATTTGGAATCATACGACCTGGATGGAG
  TGGGACAGAGAGATTAACAACTATACGTCCCTTATTCACTCCCTGATTGAAGAAAGTCAGAATCAGCAGG
  AGAAGAACGAACAAGAACTGCTTGAACTTGACAAGTGGGCCTCCCTTTGGAATTGGTTCAATATCACTAA
  TTGGCTGTGGTATATAAAGCTGTTCATCATGATTGTGGGGGGCTTGGTCGGGTTGCGGATCGTTTTCGCA
  GTGCTGTCAATCGTGAATAGGGTTCGCCAGGGTTACTCCCCTCTGAGCTTTCAGACTCACCTCCCTACCC
  CGAGAGGACCTGACAGGCCCGAGGGCATTGAAGAAGAGGGGGGAGAGAGGGACAGAGACAGATCCATCAG
  GCTGGTGAACGGGTCACTTGCCCTGATATGGGATGATCTGAGAAGCCTTTGCTTGTTCTCCTATCACAGG
  TTGCGGGACTTGCTCCTGATAGTGACCAGGATTGTTGAGTTGCTGGGGCGCCGGGGTTGGGAGGCCCTGA
  AGTACTGGTGGAATTTGCTGCAGTACTGGTCACAAGAGCTGAAAAATAGCGCCGTTTCTCTTCTGAATGC
  AACTGCCATTGCAGTGGCCGAGGGCACCGATAGGGTTATCGAAGTGGTTCAGGGCGCCTGTAGGGCTATC
  CGACATATTCCGCGCCGCATCCGCCAGGGGCTGGAGCGCATCCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101721 representing NP_057856
  Red=Cloning sites Green=Tags
  
  MRVKEKYQHLWRWGWRWGTMLLGMLMICSATEKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWA
  THACVPTDPNPQEVVLVNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNT
  NSSSGRMIMEKGEIKNCSFNISTSIRGKVQKEYAFFYKLDIIPIDNDTTSYKLTSCNTSVITQACPKVSF
  EPIPIHYCAPAGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSVNFTDN
  AKTIIVQLNTSVEINCTRPNNNTRKRIRIQRGPGRAFVTIGKIGNMRQAHCNISRAKWNNTLKQIASKLR
  EQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNSTWFNSTWSTEGSNNTEGSDTITLPCRI
  KQIINMWQKVGKAMYAPPISGQIRCSSNITGLLLTRDGGNSNNESEIFRPGGGDMRDNWRSELYKYKVVK
  IEPLGVAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIE
  AQQHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWME
  WDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFA
  VLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSLALIWDDLRSLCLFSYHR
  LRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELKNSAVSLLNATAIAVAEGTDRVIEVVQGACRAI
  RHIPRRIRQGLERILL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 2568 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001802.1, NP_057856
• RefSeq ORF: 2568
• Locus ID: 155971
• MW: 97.2 kDa