vpr (NC_001802) Virus Tagged ORF Clone

CAT#: VC101720

Myc-DDK-tagged ORF clone of viral ORF for Vpr [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057852

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: vpr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101720 represents NCBI reference of NP_057852 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAGGCTCCAGAAGACCAAGGGCCTCAGAGAGAGCCTCACAACGAGTGGACACTGGAGCTGCTGG
  AGGAACTGAAGAACGAGGCTGTGAGGCATTTCCCCCGCATATGGTTGCATGGCCTTGGGCAGCACATTTA
  CGAAACATACGGAGATACGTGGGCTGGTGTGGAGGCAATCATCCGCATTCTGCAGCAGCTGCTGTTCATC
  CACTTCCGGATCGGCTGTCGACACTCCCGCATCGGGGTGACTCGGCAACGAAGGGCGAGAAATGGCGCGT
  CAAGGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101720 representing NP_057852
  Red=Cloning sites Green=Tags
  
  MEQAPEDQGPQREPHNEWTLELLEELKNEAVRHFPRIWLHGLGQHIYETYGDTWAGVEAIIRILQQLLFI
  HFRIGCRHSRIGVTRQRRARNGASRS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 288 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001802.1, NP_057852
• RefSeq ORF: 288
• Locus ID: 155807
• MW: 11.3 kDa