reverse transcriptase p51 subunit (NC_001802) Virus Tagged ORF Clone

CAT#: VC101737

Myc-DDK-tagged ORF clone of viral ORF for reverse transcriptase p51 subunit [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_789739

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: reverse transcriptase p51 subunit
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101737 represents NCBI reference of NP_789739 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAATTAGTCCAATTGAAACAGTTCCAGTTAAGCTTAAACCAGGAATGGACGGCCCTAAGGTTAAGC
  AGTGGCCACTGACCGAGGAAAAAATCAAAGCTCTGGTGGAAATTTGCACCGAGATGGAGAAGGAGGGAAA
  AATTTCAAAGATTGGTCCTGAGAACCCGTATAACACTCCCGTATTTGCTATTAAGAAAAAAGACTCTACA
  AAATGGAGGAAACTGGTGGATTTTCGAGAACTCAATAAAAGGACCCAGGATTTCTGGGAGGTGCAGCTCG
  GGATCCCACACCCAGCGGGCTTGAAAAAGAAGAAGAGTGTAACTGTGCTTGACGTGGGCGACGCATATTT
  TTCAGTACCACTCGATGAAGACTTCAGGAAGTACACGGCGTTTACCATTCCTTCAATCAACAATGAGACA
  CCGGGGATCCGGTACCAGTATAACGTCCTCCCCCAAGGGTGGAAAGGGTCCCCTGCCATATTTCAGTCTA
  GCATGACAAAAATTCTCGAACCATTTAGGAAACAGAATCCAGACATCGTTATCTATCAGTATATGGATGA
  TCTGTACGTTGGCAGCGACCTTGAGATTGGACAACACCGAACCAAAATTGAGGAGCTGCGACAGCATCTG
  CTGAGATGGGGCCTCACTACTCCAGACAAAAAGCATCAGAAAGAGCCTCCGTTTCTGTGGATGGGGTATG
  AGCTGCACCCAGACAAATGGACTGTTCAGCCCATTGTGCTCCCGGAAAAAGATTCTTGGACCGTTAATGA
  TATCCAGAAACTTGTAGGGAAGCTGAACTGGGCATCCCAGATCTATCCCGGGATCAAGGTACGCCAGCTC
  TGCAAACTCCTCCGCGGCACAAAGGCACTTACTGAAGTGATCCCACTGACGGAAGAGGCGGAACTCGAGC
  TCGCAGAAAATCGAGAGATCCTGAAGGAGCCCGTTCACGGCGTGTACTACGATCCTTCCAAGGACCTGAT
  TGCAGAGATTCAGAAGCAGGGACAGGGACAGTGGACCTATCAGATCTACCAGGAGCCTTTTAAGAATCTC
  AAGACAGGAAAATACGCCCGAATGCGAGGAGCCCACACCAATGATGTTAAGCAACTGACCGAGGCGGTCC
  AGAAGATCACCACAGAAAGCATCGTGATCTGGGGCAAAACTCCGAAGTTCAAACTCCCGATCCAGAAGGA
  GACTTGGGAAACTTGGTGGACCGAGTACTGGCAGGCCACTTGGATACCGGAGTGGGAGTTCGTTAACACA
  CCCCCTCTCGTAAAGCTGTGGTACCAGTTGGAGAAGGAGCCGATCGTCGGCGCTGAAACTTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101737 representing NP_789739
  Red=Cloning sites Green=Tags
  
  MPISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKDST
  KWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNET
  PGIRYQYNVLPQGWKGSPAIFQSSMTKILEPFRKQNPDIVIYQYMDDLYVGSDLEIGQHRTKIEELRQHL
  LRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKDSWTVNDIQKLVGKLNWASQIYPGIKVRQL
  CKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNL
  KTGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNT
  PPLVKLWYQLEKEPIVGAETF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001802
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001802.1, NP_789739
• RefSeq ORF: 1323
• MW: 51.5 kDa