Secondary Antibodies

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Monkey IgA + IgG + IgM goat polyclonal antibody, Biotin

Applications Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/50,000-1/100,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Monkey
Conjugation Biotin
Immunogen Monkey IgG, IgA and IgM whole molecule.

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/100,000-1/200,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Conjugation HRP
Immunogen Guinea Pig IgG whole molecule.

Human IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/2,000.
Reactivities Human
Immunogen Human IgG whole molecule.

Rabbit IgG (H+L chain) goat polyclonal antibody, TRITC, Lyophilized

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
IF Microscopy: 1/1,000-1/5,000.
Reactivities Rabbit
Conjugation TRITC
Immunogen Rabbit IgG, whole molecule

Human IgG (adsorbed F(ab)2 Fragment) goat polyclonal antibody, Aff - Purified

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1:10,000-1:50,000.
Western Blot: 1:1,000-1:5,000.
Immunohistochemistry: 1:500-1:2,000.
Immunogen Human IgG whole molecule

Bovine IgG (H+L chain) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (1/5,000-1/100,000, Western or dot blot), ELISA (1/10,000-1/200,000), Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/500-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Reactivities Bovine
Conjugation HRP
Immunogen Bovine IgG whole molecule.

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications ELISA: 1/500-1/1000.
Immunohistochemistry on Frozen Sections: 1/50.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG

Canine IgA goat polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (Reducing conditions).
Immunodiffusion.
Immunohistochemistry on Frozen Sections: 1/200-1/500.
Reactivities Canine
Immunogen Purified Canine IgA.

Canine IgM goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Flow Cytometry.
Immunodiffusion.
Immunohistochemistry on Frozen Sections.
Reactivities Canine
Immunogen Purified Canine IgM.

Biotin Conjugated Goat Anti-Mouse IgM

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Mouse IgM
Conjugation Biotin
Immunogen Mouse IgM, whole molecule

Biotin Conjugated Goat Anti-human IgM

Applications WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000
Reactivities Human IgM
Conjugation Biotin
Immunogen Human IgM, whole molecule

FITC Conjugated Goat Anti-human IgA

Applications FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64
Reactivities Human IgA
Conjugation FITC
Immunogen Human IgA, whole molecule

Peroxidase Conjugated Goat Anti-human IgA (alpha-chain specific)

Applications WB: 1:3,000-10,000; ELISA: 1:20,000-40,000
Reactivities Human IgA
Conjugation HRP
Immunogen Human IgA, whole molecule

Human IgA (alpha chain) goat polyclonal antibody, Biotin

Applications Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody
based assays using streptavidin or avidin conjugates:
ELISA: 1/60,000-1/600,000
Western Blot: 1/2,000-1/10,000
Immunohistochemistry: 1/1,000-1/5,000
Reactivities Human
Conjugation Biotin
Immunogen Human IgA (alpha chain)

Rat IgA goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Immunofluorescence.
Reactivities Reptiles, Rat

Rat IgG1 goat polyclonal antibody, Purified

Applications ELISA: 1-10 µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Immunogen Goats with highly purified antigen.

Rat IgM goat polyclonal antibody, Purified

Applications ELISA 1ug/ml - 10ug/ml, Western Blotting, Flow Cytometry
Reactivities Rat

Mouse IgG (adsorbed) goat polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Mouse

Mouse IgG1 goat polyclonal antibody, HRP

Applications ELISA: 1/4000-1/8000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry onParaffin Sections.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG1 paraproteins.

Mouse IgG2a goat polyclonal antibody

Applications ELISA: 1/2000 - 1/4000.
Reactivities Mouse
Immunogen Mouse IgG2a paraproteins

Mouse IgG2a goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/10.
Immunofluorescence: 1/10.
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG2a paraproteins

Mouse IgG2b goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.  
Western Blot. 
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG2b paraproteins.

Mouse IgG2b goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10. 
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG2b paraproteins.

Mouse IgA goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse, Rat
Conjugation FITC
Immunogen Mouse IgA paraproteins

Mouse IgA goat polyclonal antibody, HRP

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA: 1/4000 - 1/8000.
Western Blot.
Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgA paraproteins

Mouse IgM goat polyclonal antibody

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA: 1/2000 - 1/4000.
Western Blot.
Reactivities Mouse
Immunogen Mouse IgM paraproteins

Human IgD (heavy chain) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC
Immunogen Human IgD paraproteins.

Human IgD (F(ab)2 Fragment) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC
Immunogen Human IgD

Chicken IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40, depending on the method used.
Reactivities Chicken
Conjugation FITC
Immunogen Purified IgM isolated from chicken serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Monkey IgA (Secretory component) goat polyclonal antibody, Serum

Applications Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes.
Can be used in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence secretory component in monkey serum or other body fluids and to determine its concentration.
Recommended dilutions:
Immunoelectrophoresis: use 2 μl serum or equivalent against 120 μl antiserum.
Double radial immunodiffusion: use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
Reactivities Chimpanzee, Monkey
Immunogen Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free secretory component isolated from pooled rhesus monkey milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Feline IgG (H+L chain) goat polyclonal antibody, Serum

Applications Can be used:
• In precipitating techniques as Immunoelectrophoresis and Double Radial Immunodiffusion to identify the presence of IgG.
• As secondary antibody to precipitate the immunoglobulin in normal Cat serum.
• To prepare an immunoadsorbent for the production of Ig-free Cat serum, plasma or non-Ig preparations.
Directions for use:
Immunoelectrophoresis: Use 2 μl or equivalent against 120 μl antiserum. 
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells.
Reactivities Feline
Immunogen Purified normal IgG isolated from pooled cat serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2000.
Reactivities Canine
Immunogen Purified IgA isolated from dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgG (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000.
Reactivities Canine
Immunogen Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Canine IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/3000.
Reactivities Canine
Immunogen Purified IgM isolated from Dog serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, Biotin

Applications ELISA.
Western Blot (1/5000).
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA using HRP-labeled streptavidin. A 1/500,000 dilution was found to generate an O.D of 1.0 in a 30 minute reaction with Tetramethylbenzidine as the substrate.
Reactivities Chicken
Conjugation Biotin
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.

Mouse IgG3 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation FITC
Immunogen Mouse IgG3 paraproteins

Mouse IgG3 goat polyclonal antibody, HRP

Applications

ELISA: 1/4000-1/8000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.

Reactivities Mouse
Conjugation HRP
Immunogen Mouse IgG3 paraproteins.

Mouse IgA (alpha chain specific) goat polyclonal antibody, AP

Applications

ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.

Reactivities Mouse
Conjugation AP
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.

Mouse IgA (alpha chain specific) goat polyclonal antibody, Texas Red

Applications FLISA: 1/100-1/400.
Flow Cytometry.
Immunohistochemistry.
Reactivities Mouse
Conjugation Texas Red
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, FITC

Applications

FLISA.
Immunofluoresecence: ≤ 1 μg/106 cells.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation FITC
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Immunoblotting.
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, Texas Red

Applications

FLISA: 1/100–1/400.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation Texas Red
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.

Mouse IgG goat polyclonal antibody, AP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Immunogen Purified Mouse IgG fraction

Mouse IgG goat polyclonal antibody, HRP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation HRP
Immunogen Purified Mouse IgG fraction

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, AP, Aff - Purified

Applications Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/10,000-1/40,000.
Western Blot: 1/200-1/2,000. 
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP
Immunogen Native Mouse IgG1 heavy chain Protein.

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, Biotin, Aff - Purified

Applications Anti-Mouse IgG1 Biotin Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation Biotin
Immunogen Native Mouse IgG1 heavy chain Protein.

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, HRP, Aff - Purified

Applications Mouse IgG1 Peroxidase Secondary antibody reagents are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/20,000. 
Immunohistochemistry: 1/1,000-1/5,000.
Reactivities Mouse
Conjugation HRP
Immunogen Native Mouse IgG1 heavy chain Protein.

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, Aff - Purified

Applications Anti-Mouse IgG1 secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
Recommended Dilutions:
ELISA: 1/40,000-1/200,000.
Western Blot: 1/4,000-1/20,000. 
Immunohistochemistry: 1/2,000-1/10,000.
Reactivities Mouse
Immunogen Native Mouse IgG1 heavy chain Protein.

Guinea pig IgG (H+L chain), adsorbed goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Conjugation FITC
Immunogen Guinea Pig IgG whole molecule.

Human IgG (H+L chain), adsorbed goat polyclonal antibody, HRP

Applications
ELISA: 1/200,000. 
Western Blot: 1/1,000-1:5,000.
Immunohistochemistry: 1/500-1/2,500
Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
Conjugation HRP
Immunogen Human IgG whole molecule.