Germany
Japan
United Kingdom
China
Mouse IgG (H+L chain) porcine polyclonal antibody, Serum
| Reactivities | Mouse |
Secondary Antibodies
DownloadCanine IgA (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of dog origin known to be of the IgA isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgA in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/500. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Biotin |
| Immunogen | Purified normal IgA isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgA (Fc specific) goat polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgA from serum or plasma. Directions for use: In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. |
| Reactivities | Canine |
| Immunogen | Highly purified normal IgA isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgG (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical use for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Canine |
| Conjugation | Biotin |
| Immunogen | Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgG (Fc specific) goat polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgG from serum or plasma. Directions for use: In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. |
| Reactivities | Canine |
| Immunogen | Highly purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgG (H+L chain) goat polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal dog serum; to prepare an immunoadsorbent for the production of Ig-free dog serum, plasma or non-Ig preparations. Directions for use: In immunoelectrophoresis use 2 ml or equivalent against 120 ml antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ml antiserum in a 3 mm diameter centre well and 2 ml serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Canine |
| Immunogen | Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in dog serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/1000 and 1/4000. |
| Reactivities | Canine |
| Conjugation | Biotin |
| Immunogen | Purified normal IgM isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgM (Fc specific) goat polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in dog serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgM from serum or plasma. Directions for use: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl in a 3 mm diameter center well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Canine |
| Immunogen | Highly purified normal IgM isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgM (Fc specific) goat polyclonal antibody, Serum
| Applications | Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgM from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available. Directions for use: In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. |
| Reactivities | Mouse |
| Immunogen | Highly purified homogenous IgM isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgG rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Immunodiffusion. For Ouchterlony: Sample Size: 15 µl Distance between wells: 5 mm Test time: 4 days at 37°C Antigens tested: Normal human serum, purified human IgG, normal mouse serum. Results: Titre with NHS > 1:1000. |
| Reactivities | Human |
Mouse IgG rabbit polyclonal antibody, Serum
| Reactivities | Mouse |
Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP
| Applications | ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate. Western Blot (1/1000). Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough. Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA. |
| Reactivities | Chicken |
| Conjugation | AP |
| Immunogen | Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. |
Goat IgG (H+L chain) chicken polyclonal antibody, FITC
| Applications | Immunocytochemistry (1/1000). Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues. |
| Reactivities | Goat |
| Conjugation | FITC |
| Immunogen | Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant. Production. After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate. |
Human IgG (H+L chain) chicken polyclonal antibody, FITC
| Applications | Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues. |
| Reactivities | Human |
| Conjugation | FITC |
| Immunogen | Purified, non-denatured Human IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains) and then conjugated with Fluorescein isothiocyanate. |
Mouse IgG (H+L chain) chicken polyclonal antibody, FITC
| Applications | Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues. |
| Reactivities | Mouse |
| Conjugation | FITC |
| Immunogen | Purified, non-denatured Mouse IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Mouse IgG's were affinity-purified over a column conjugated with purified Mouse IgG (containing both heavy and light chains) and conjugated to Fluorescein Isothiocyanate. |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, BGAL
| Applications | ELISA: 1/500. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | BGAL |
| Immunogen | Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins. |
Mouse IgM (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used: In precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgM in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgM from serum or plasma. This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available. Recommended Working Dilutions: Immunoelectrophoresis: use 2 μl or equivalent against 120 μl antiserum. Double Radial Immunodiffusion (Ouchterlony): a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre: 1/32 when tested against pooled normal mouse serum in agar-block immunodiffusion titration. |
| Reactivities | Mouse |
| Immunogen | Highly purified homogenous IgM isolated from Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgM (Fc specific) goat polyclonal antibody, Serum
| Applications | In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgM from serum or plasma. Recommended Working Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl in a 3 mm diameter center well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Precipitin titre: 1/32 when tested against pooled normal Swine serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Immunogen | Highly purified normal IgM isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgG (Fc specific) sheep polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in dog serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of dog IgG from serum or plasma. The antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive techniques. For such uses labelled and unlabelled cytochemical grade reagents raised in goats meeting the necessary additional specificity and performance requirements are available. Directions for Use: Immunoelectrophoresis: Use 2 μl serum or equivalent against 120 μl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Precipitin titre: 1/16 when tested against pooled normal dog serum in agar-block immunodiffusion titration. |
| Reactivities | Canine |
| Immunogen | Highly purified normal IgG isolated from pooled Dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgA (Fc specific) sheep polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in guinea pig serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of guinea pig IgA from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. This does not exclude such use if [roper controls are included. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/40 when tested against normal guinea pig serum in agar block titration. |
| Reactivities | Guinea Pig |
| Immunogen | Highly purified normal IgA isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Guinea Pig IgG1 (subclass specific) sheep polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in guinea pig serum and other body fluids or to determine its concentration. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/16 when tested against normal guinea pig serum in agar block titration. |
| Reactivities | Guinea Pig |
| Immunogen | Highly purified normal IgG1 isolated from pooled guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Equine IgM rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgM from serum or plasma. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/32 when tested against normal Horse serum in agar block titration. |
| Reactivities | Equine |
| Immunogen | Highly purified normal IgM isolated from pooled Horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Equine IgA rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in horse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of horse IgA from serum or plasma. Directions for Use: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: Precipitin titre not less than 1/32 when tested against normal Horse serum in agar block titration. |
| Reactivities | Equine |
| Immunogen | Highly purified normal IgA isolated from pooled Horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgG (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal sheep serum, to prepare an immunoadsorbent for the production of Ig-free sheep serum, plasma or non-Ig preparations. Recommended Working Dilutions: Immunoelectrophoresis: use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Purified normal IgG isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in sheep serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of sheep IgM from serum or plasma. Recommended Working Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Single Radial Immunodiffusion and electroimmunodiffusion: Use 0.5 to 1.0 percent antiserum in the agar gel. Antibody titre: 1/16 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Highly purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Sheep IgM (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgM and IgG antibodies, as secondary antibody to precipitate the immunoglobulin in normal sheep serum, to prepare an immunoadsorbent for the production of Ig-free sheep serum, plasma or non-Ig preparations. Recommended Working Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Sheep |
| Immunogen | Highly purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgA (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion (Ouchterlony and Mancini) to identify the presence of IgA in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgA from serum or plasma. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available. Recommended Working Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: 1/32 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Immunogen | Highly purified normal IgA isolated from pooled Swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Immunoprecipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG in swine serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgG from serum or plasma. The antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive techniques. For such uses labelled and unlabelled cytochemical grade reagents meeting the necessary additional specificity and performance requirements are available. Recommended Working Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Immunogen | Highly purified normal IgG isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgG (H+L chain) rabbit polyclonal antibody, Serum
| Applications | Precipitation. Can be used in immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal swine serum; to prepare an immunoadsorbent for the production of Ig-free swine serum, plasma or non-Ig preparations. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Antibody Titre: Precipitin titre not less than 1/64 when tested against normal swine serum in agar block titration. |
| Reactivities | Porcine |
| Immunogen | Purified normal IgG isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Porcine IgM (Fc specific) rabbit polyclonal antibody, Serum
| Applications | Precipitation. Can be used in precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in swine serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of swine IgM from serum or plasma. Recommended Dilutions: Immunoelectrophoresis: Use 2 µl or equivalent against 120 µl antiserum. Double Radial Immunodiffusion: Use a rosette arrangement with 10 µl antiserum in a 3 mm diameter centre well and 2 µl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Single Radial Immunodiffusion and electroimmunodiffusion: Use 0.5 to 1.0 percent antiserum in the agar gel. Antibody Titre: Precipitin titre not less than 1/32 when tested against pooled normal swine serum in agar-block immunodiffusion titration. |
| Reactivities | Porcine |
| Immunogen | Highly purified normal IgM isolated from pooled swine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilution(s): FLISA : 1:10,000 - 1:50,000 IF Microscopy : 1:1,000 - 1:5,000 Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500 |
| Conjugation | TRITC |
| Immunogen | Mouse IgG whole molecule. |
Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy: 1/1,000-1/5,000. Flow Cytometry: 1/500-1/2,500. |
| Reactivities | Goat |
| Conjugation | FITC |
| Immunogen | Goat IgG whole molecule. |
Rabbit IgG (H+L chain) sheep polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, immuno-peroxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Conjugation | HRP |
| Immunogen | Rabbit IgG whole molecule. |
Rabbit IgG (H+L chain) sheep polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000. |
| Conjugation | TRITC |
| Immunogen | Rabbit IgG, whole molecule |
Canine IgG (H+L chain) rabbit polyclonal antibody, HRP
| Applications | Immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This antibody has been assayed against 1.0 ug of Dog IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:16,000 to 1:72,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Dog IgG whole molecule |
Goat IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunofluorescence Microscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000. |
| Conjugation | FITC |
| Immunogen | Goat IgG whole molecule |
Goat IgG (H+L chain) rabbit polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy (1/1,000-1/5,000) and Flow Cytometry (1/500-1/2,500) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | TRITC |
| Immunogen | Goat IgG, whole molecule |
Hamster IgG (H+L chain), adsorbed goat polyclonal antibody, Aff - Purified
| Applications | Suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1:800,000. Western Blot: 1:5,000 - 1:50,000. Immunohistochemistry: 1:1,000 - 1:5,000. |
| Immunogen | Hamster IgG whole molecule. |
Hamster IgG (H+L chain) rabbit polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilution(s): Flow Cytometry: 1/2,000-1/10,000. IF Microscopy: 1/500-1/2,500. |
| Conjugation | FITC |
| Immunogen | Hamster IgG whole molecule. |
Equine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (1/1,000-1/10,000), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 ug of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/200,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Horse IgG F(c) fragment. |
Equine IgG (F(ab)2 specific) rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot: use at 1/1,000-1/5,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (use at 1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Horse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Horse IgG F(ab')2 fragment. |
Human IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/50,000-1/100,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:9,000 to 1:41,000 is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Human IgG whole molecule. |
Human IgG (F(ab)2 specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/25,000. Western Blot: 1/2,000-1/10,000. Immunohistochemisstry: 1/1,000-1/5,000. |
| Immunogen | Human IgG F(ab')2 fragment. |
Human IgG (F(ab)2 specific), adsorbed rabbit polyclonal antibody, FITC
| Applications | Suitable for immunomicroscopy, Fluorescent based plate assays (FLISA) and Fluorescent Western Blotting. Also Suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Recommended Dilutions: FLISA: 1/10,000-1/50,000. IF Microscopy : 1/1,000-1/5,000. |
| Conjugation | FITC |
| Immunogen | Human IgG F(ab')2 fragment |
Monkey IgM (Mu chain specific) goat polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | FITC |
| Immunogen | Monkey IgM µ mu chain. |
Monkey IgM (Mu chain specific) goat polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Conjugation | TRITC |
| Immunogen | Monkey IgM µ mu chain. |
Monkey IgM (Mu chain specific) goat polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. FLISA: 1:10,000-1:50,000 IF: 1:1,000-1:5,000 |
| Conjugation | Texas Red |
| Immunogen | Monkey IgM µ (mu) heavy chain.nm |
Monkey IgA (alpha chain specific) goat polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. FLISA: 1:10,000-1:50,000 IF: 1:1,000-1:5,000 |
| Conjugation | FITC |
| Immunogen | Monkey IgA α chain. |
Mouse IgG (H+L chain) chicken polyclonal antibody, TRITC
| Applications | Immunomicroscopy. Flow Cytometry or FACS analysis. |
| Conjugation | TRITC |
| Immunogen | Mouse IgG whole molecule. |
Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product. |
| Conjugation | HRP |
| Immunogen | Mouse IgG whole molecule. |
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Clonalities
- Polyclonal