Germany
Japan
United Kingdom
China
Human IgM (Mu chain, Fc-region) mouse monoclonal antibody, clone 2A6
| Reactivities | Human |
Antibodies
View as table DownloadChicken IgG / Chicken IgY mouse monoclonal antibody, clone 9B4, Purified
| Applications | Western Blot: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. ELISA: 0.1 µg/ml. Immunoprecipitation: 1-10 µg. |
| Reactivities | Chicken |
Human IgA1 mouse monoclonal antibody, clone NI 69-11 (A89-036), Azide Free
| Applications | To identify the presence of IgA1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA1, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200 ELISA: from 1/500 upwards Western blot: 1/3,000-1/50,000. |
| Reactivities | Human |
USD 550.00
2 Weeks
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Purified
| Applications | To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG1, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
USD 680.00
2 Weeks
Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), TRITC
| Applications | To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. General Recommended Dilutions: Histochemical Use: 1/10-1/50. ELISA: from 1/200 upwards. Western blot: from 1/400 upwards. |
| Reactivities | Human |
| Conjugation | TRITC |
USD 550.00
2 Weeks
Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Purified
| Applications | To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG2, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
USD 680.00
2 Weeks
Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), HRP
| Applications | To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
USD 680.00
2 Weeks
Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), HRP
| Applications | To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
USD 550.00
2 Weeks
Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Purified
| Applications | To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG4, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
USD 680.00
2 Weeks
Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, HRP
| Applications | To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/500 ELISA: from 1/1000 upwards Western blot: from 1/2000 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
Human IgM mouse monoclonal antibody, clone NI 179, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/250 upwards. Western blot: from 1/500 upwards. |
| Reactivities | Human |
| Conjugation | HRP |
USD 680.00
2 Weeks
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Biotin
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/20 upwards ELISA: form 1/25 upwards Western blot: from 1/50 upwards. |
| Reactivities | Human |
| Conjugation | Biotin |
USD 680.00
2 Weeks
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), FITC
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques Immunofluorescence staining techinqiues. General Recommended Dilutions: Histochemical Use: from 1/10 upwards. |
| Reactivities | Human |
| Conjugation | FITC |
USD 450.00
2 Weeks
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Ascites
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/50-1/200. ELISA: form 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM260, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone IM373, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Recommended Positive Control: 293T, Raji or hPBL cells, Tonsil or Spleen. |
| Reactivities | Human |
Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgG (heavy chain) mouse monoclonal antibody, clone SPM556, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Human IgM (heavy chain) mouse monoclonal antibody, clone SPM557, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
IGHG1 (heavy chain) mouse monoclonal antibody, clone IG266, Purified
| Applications | ELISA: Use Antibody without BSA for Coating. Flow Cytometry: 0.5-1 μg/106 cells. Immunofluorescence: 0.5-1 μg/ml. Western Blot: 0.5-1 μg/ml. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 μg/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen. |
| Reactivities | Human |
Sheep/Goat IgG donkey polyclonal antibody, HRP
| Applications | ELISA: 1/12800-1/25600. Western Blot: 1/20000. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Frozen Sections: 1/50-1/100. |
| Reactivities | Goat, Sheep |
| Conjugation | HRP |
| Immunogen | Sheep IgG |
Duck IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. Can be used to prepare conjugates of the user’s own choice, to prepare an insoluble Immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/200-1/4,000. Immunohistochemistry: 1/50-1/250. Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Antibody titre: Precipitin titre 1/16 when tested against pooled normal Duck serum in agar-block immunodiffusion titration. |
| Reactivities | Duck |
| Immunogen | Purified IgM isolated from Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | Suitable for use in Immunocytochemical and iImunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgM in duck serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000. Immunohistochemistry and Immunocytochemistry: 1/50-1/250. Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Duck |
| Conjugation | Biotin |
| Immunogen | Purified IgM isolated from Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Duck IgM (Fc specific) goat polyclonal antibody, FITC
| Applications | Suitable for use in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of duck origin known to be of the IgM isotype in the middle layer of the indirect test procedure. Recommended Dilutions: 1/10-1/40. Note: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Duck |
| Conjugation | FITC |
| Immunogen | Purified normal IgM isolated from pooled Duck serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Human IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled secondary antibody for Indirect detection of IgE in Human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Human |
| Immunogen | Purified homogenous IgE isolated from Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled secondary antibody for indirect detection of IgE in mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Recommended working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays. |
| Reactivities | Mouse |
| Immunogen | Purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgE (Fc specific) goat polyclonal antibody, Biotin
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
| Immunogen | Purified homogenous IgE isolated from Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rat IgE (Fc specific) goat polyclonal antibody, Azide Free
| Applications | This antibody can be used: • As unlabelled secondary antibody for indirect detection of IgE in Rat cell, tissue substrates and body fluids. • In Immunofluorescence and immunoenzyme assay methods. • For the production of immunoconjugates with a selected marker. • To prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier. • As catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. Recommended Dilutions: Histochemistry and Cytochemistry: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. |
| Reactivities | Rat |
| Immunogen | Purified homogenous IgE isolated from Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: Histochemistry: 1/50 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/3000. Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration. |
| Reactivities | Rabbit |
| Immunogen | Purified IgM isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rabbit IgA (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: Histochemistry: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2500. Antibody titre: Precipitin titre not less than 1/32 when tested against pooled normal rabbit serum in agar-block immunodiffusion titration. |
| Reactivities | Rabbit |
| Immunogen | Purified IgA isolated from rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure |
Rat IgG2b (subclass specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgG2b at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended working dilutions: Histochemistry: 1/100 - 1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Rat |
| Immunogen | Pools of purified homogenous IgG2b isolated from pooled rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rat IgG2ab (subclass specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended working dilutions: Histochemistry: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Rat |
| Immunogen | Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Rat IgG2ab (subclass specific) goat polyclonal antibody, Biotin
| Applications | Can be used: In immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates. To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays between: 1/2.000-1/10,000. |
| Reactivities | Rat |
| Conjugation | Biotin |
| Immunogen | Pools of highly purified homogenous IgG2a and IgG2b isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Mouse IgG3 (subclass specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended Working Dilutions: Histochemistry: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5.000. Performance testing: Titre, specificity and reactivity of the subclass specific cross-adsorbed IgG fraction is further evaluated in a number of highly sensitive non-precipitating antibody-binding assay systems. These performance tests include direct single and double identification of cIg in mouse cell and tissue substrates and their evaluation as second antibody in the analysis of human cells and tissues after reacting with a primary monoclonal mouse antibody to a human antigen. The quantitative specific recognition ability is verified in double staining procedures together with reference reagents of known specificity and reactivity. |
| Reactivities | Mouse |
| Immunogen | Pools of purified homogenous IgG3 isolated from pooled Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Bovine IgM (Fc specific) rabbit polyclonal antibody, Azide Free
| Applications | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase Immunochemistry. Recommneded Dilutions: Histochemistry: 1/50-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/2,000. |
| Reactivities | Bovine |
| Immunogen | Purified normal IgM isolated from pooled Bovine serum. Feund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Azide Free
| Applications | This IgG fraction allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/500. In ELISA and comparable non-precipitating antibody-binding assays are between 1/1000 and 1/5000. |
| Reactivities | Monkey |
| Immunogen | Highly purified IgG and pools of homogenous IgA and IgM isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA + IgG + IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions: For histochemical and cytochemical use are usually between 1/100 and 1/500. In ELISA and comparable non-precipitating antibody-binding assays are between 1/1000 and 1/5000. |
| Reactivities | Monkey |
| Immunogen | Highly purified immunoglobulin fractions containing IgG, IgA and IgM isolated from monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA (Fc specific) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/2500. |
| Reactivities | Monkey |
| Immunogen | Purified IgA isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (H+L chain) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Monkey |
| Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgM (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended Working Dilutions: In Histochemistry are usually between 1/100 and 1/500. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Monkey |
| Immunogen | Purified homogenous IgM isolated from monkey serum. Immunization with intact (19S) and split IgM (7S). Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA + IgG + IgM (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. |
| Reactivities | Monkey |
| Immunogen | Highly purified IgG, IgA and IgM isolated from Rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgG (H+L chain) rabbit polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: Histochemistry: 1/50 and 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Monkey |
| Immunogen | Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Monkey IgA (Secretory component) goat polyclonal antibody, Azide Free
| Applications | Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Working dilutions: Histochemistry: 1/50 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/200 - 1/1000. |
| Reactivities | Chimpanzee, Monkey |
| Immunogen | Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Canine IgG (H+L chain) goat polyclonal antibody, Azide Free
| Applications | Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Recommended working dilutions: In histochemistry are usually between 1/50 and 1/250. In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000. |
| Reactivities | Canine |
| Immunogen | Purified normal IgG isolated from pooled dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
Goat IgG (H+L chain) chicken polyclonal antibody, HRP
| Applications | ELISA: a 1/75,000 dilution gave an O.D. of 1.0 in a 15 minute reaction using Tetramethylbenzidine as substrate. Western Blot (1/5000). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in Agarose gels and ELISA analysis. |
| Reactivities | Goat |
| Conjugation | HRP |
| Immunogen | Purified, non-denatured Goat IgG (containing both heavy and light chains) emulsified in Freund's adjuvant. Production: After multiple injections, eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-goat IgG's were affinity-purified over a column conjugated with purified Goat IgG (containing both heavy and light chains) and then conjugated with Horseradish Peroxidase. |
Human IgG (H+L chain) chicken polyclonal antibody, Aff - Purified
| Applications | ELISA. Western Blot (1/1000). Immunocytochemistry. Immunohistochemistry (1/250). Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose. |
| Reactivities | Human |
| Immunogen | Purified, non-denatured Human IgG (containing both heavy and light chains). Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains). |
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