Antibodies

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Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), AP, Purified

Applications ELISA: 1/1000-1/2000 (See also Ref.2-9). 
FLISA
Applications Reported in Literature: 
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation AP

IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections:  reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry:
reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP

GFP goat polyclonal antibody, AP

Applications ELISA, IHC, WB
Reactivities A. victoria
Conjugation AP
Immunogen The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/3,000-1/25,000.
Western blot: 1/1,000-1/4,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Rabbit
Conjugation AP
Immunogen Rabbit IgG, whole molecule.

c-Myc (MYC) mouse monoclonal antibody, clone 9E10, AP

Applications ELISA, IHC, WB
Reactivities Human
Conjugation AP

Mouse IgG1 goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG1 paraproteins.

Mouse IgG (Fc specific) goat polyclonal antibody, AP

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/2,000-1/100,000
Western blot: 1/500-1/2,500
Immunohistochemistry: 1/200-1/1,000.
Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code #NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/5,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG F(c) fragment

Digoxigenin mouse monoclonal antibody, clone 21H8, AP

Applications ELISA, IHC, ISH, SB, WB
Conjugation AP

Mouse IgG (H+L chain) sheep polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions: This product has been assayed against 1.0 �g of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Conjugation AP
Immunogen Mouse IgG whole molecule.

Goat IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/13,000.
Western blot: 1/500-1/2,500. 
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Goat IgG whole molecule.

Bovine IgG1 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG1.

FITC goat polyclonal antibody, AP

Applications ELISA, ID, IHC, WB
Conjugation AP
Immunogen Fluorescein conjugated to Goat IgG

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Immunogen Pooled antisera from Goats hyperimmunized with Mouse IgM, IgG and IgA paraproteins.

Bovine IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product.
Reactivities Bovine
Conjugation AP
Immunogen Bovine IgG whole molecule.

Bovine IgG (Fc specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation AP
Immunogen Bovine IgG F(c) fragment.

Bovine IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,600 to 1:11,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation AP
Immunogen Bovine IgG F(ab')2 fragment.

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, AP

Applications Immunohistochemistry on frozen sections.
ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation AP

Mouse IgG2b goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.  
Western Blot. 
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG2b paraproteins.

Sheep/Goat IgG donkey polyclonal antibody, AP

Applications Western blot.
ELISA: 1/2000-1/5000.
Immunohistochemistry on Frozen Sections.
Reactivities Goat, Sheep
Conjugation AP
Immunogen Purified IgG from a Sheep serum pool

GST-Tag goat polyclonal antibody, AP

Applications ELISA, IP, WB
Reactivities Schistosoma japonicum
Conjugation AP
Immunogen Glutathione-S-Transferase [Schistosoma japonicum]

R-Phycoerythrin goat polyclonal antibody, AP

Applications ELISA, IHC, WB
Conjugation AP
Immunogen Highly purified R-Phycoerythrin from the seaweed gracila

KLH rabbit polyclonal antibody, AP

Applications ELISA, IHC, WB
Conjugation AP
Immunogen KLH (Keyhole Limpet Hemocyanin)

Biotin rabbit polyclonal antibody, AP

Applications ELISA, IHC, WB
Conjugation AP
Immunogen Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

FITC F(ab)2 Fragment goat polyclonal antibody, AP

Applications ELISA, IHC, WB
Conjugation AP
Immunogen Fluorescein conjugated to Goat IgG

Biotin (F(ab')2 fragment) goat polyclonal antibody, AP

Applications ELISA, IHC, WB
Conjugation AP
Immunogen Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Mouse IgA (alpha chain specific) goat polyclonal antibody, AP

Applications

ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.

Reactivities Mouse
Conjugation AP
Immunogen Pooled antisera from goats hyperimmunized with Mouse IgA paraproteins.

Mouse IgG goat polyclonal antibody, AP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation AP
Immunogen Purified Mouse IgG fraction

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, AP, Aff - Purified

Applications Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/10,000-1/40,000.
Western Blot: 1/200-1/2,000. 
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP
Immunogen Native Mouse IgG1 heavy chain Protein.

Mouse IgG (H+L chain) rabbit polyclonal antibody, AP

Applications Suitable for use in Immunoblotting (Western or dot blot), ELISA, Immunohistochemistry and other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/2,000-1/10,000.
Western blot: 1/500-1/2,500.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Mouse IgG whole molecule

Human IgM (Mu chain specific) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other AP-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/1,000-1/3,000.
Western blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Conjugation AP
Immunogen Human IgM μ mu chain

Cyanine Dyes sheep polyclonal antibody, AP

Applications ELISA, IHC, WB
Conjugation AP
Immunogen A mixture of Cy3 and Cy5 conjugated KLH

GFP mouse monoclonal antibody, clone 9F9.F9, AP

Applications ELISA, IHC, WB
Reactivities A. victoria
Conjugation AP

Biotin goat polyclonal antibody, AP

Applications ELISA, IHC, WB
Conjugation AP
Immunogen Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, AP

Applications ELISA: 1/500-1/1,000.
Western blotting.
Dot- and Slot-Immunoblotting
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation AP

Bovine IgG2 sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG2.

Porcine IgA goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/1000-1/10000. 
Immunohistochemistry on Frozen Sections: 1/100-1/1000.
Reactivities Porcine
Conjugation AP
Immunogen Purified Porcine IgA.

Rabbit IgG Light Chain mouse monoclonal antibody, clone SB62a, AP

Applications ELISA
Reactivities Rabbit
Conjugation AP

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, AP

Applications ELISA: 1/1,000-1/2,000.
Reactivities Human
Conjugation AP

Mouse IgG (adsorbed) goat polyclonal antibody, AP

Applications ELISA: 1/200-1/2500.
Western Blot: 1/100-1/500.
Reactivities Mouse
Conjugation AP
Immunogen Whole Mouse IgG

Human IgE goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/5000.
Reactivities Human, Monkey
Conjugation AP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate.
Western Blot (1/1000).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA.
Reactivities Chicken
Conjugation AP
Immunogen Purified Chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected.

FITC rabbit polyclonal antibody, AP

Applications ELISA, WB
Conjugation AP
Immunogen FITC-KLH conjugate.

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1/1,000 to 1/5,000 of the reconstitution concentration is suggested for this product. 
Recommended Dilutions: 
ELISA: 1/2000-1/10000. 
Western Blot: 1/500-1/2500. 
Immunochemistry: 1/200-1/1000.
Reactivities Mouse
Conjugation AP
Immunogen Mouse IgG F(c) fragment.

Bovine IgG sheep polyclonal antibody, AP

Applications

ELISA: 1/5000-1/50000.
Western Blot: 1/5000-1/50000.

Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgG.

Bovine IgM sheep polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/5000-1/50000.
Reactivities Bovine
Conjugation AP
Immunogen Purified Bovine IgM.

Porcine IgG F(c) specific goat polyclonal antibody, AP

Applications ELISA: 1/5000-1/50000.
Reactivities Porcine
Conjugation AP
Immunogen Purified Porcine IgG

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, AP

Applications ELISA: 1/500-1/1000.
Reactivities Mouse
Conjugation AP

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone SB73a, AP

Applications ELISA: 1/1000-1/2000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation AP

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, AP

Applications ELISA: 1/1000-1/2000.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation AP