nonstructural protein NS2A (NC_001474) Virus Tagged ORF Clone

CAT#: VC102547

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2A [Dengue virus 2], codon optimized for human cell expression, NP_739585

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein NS2A
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102547 represents NCBI reference of NP_739585 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACATGGGCAGGTCGATAACTTCTCTCTTGGCGTCCTGGGCATGGCTCTGTTTTTGGAGGAGATGC
  TGAGAACTAGAGTTGGAACAAAGCATGCCATCCTGCTTGTAGCCGTGAGCTTCGTGACATTGATTACCGG
  GAACATGTCTTTTAGGGATCTCGGGCGCGTGATGGTAATGGTCGGAGCCACTATGACTGACGATATCGGT
  ATGGGAGTCACATATCTTGCCCTTCTCGCTGCGTTTAAAGTGCGCCCTACCTTCGCTGCTGGACTGCTCC
  TCCGAAAGCTGACATCCAAGGAACTCATGATGACAACAATTGGCATCGTCCTTCTCTCCCAGTCAACAAT
  TCCAGAGACCATCCTCGAACTCACAGACGCCCTCGCGCTCGGAATGATGGTACTGAAAATGGTAAGAAAC
  ATGGAAAAATATCAACTGGCAGTCACGATTATGGCCATCCTCTGTGTACCGAACGCTGTTATTCTGCAGA
  ACGCCTGGAAAGTTAGCTGTACTATTCTCGCCGTCGTCTCAGTCTCACCCTTGCTCCTCACCTCATCTCA
  ACAAAAAACCGACTGGATTCCCTTGGCGCTGACAATAAAAGGACTGAACCCCACCGCTATCTTTTTGACA
  ACCCTTAGCCGGACTTCAAAGAAACGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102547 representing NP_739585
  Red=Cloning sites Green=Tags
  
  MGHGQVDNFSLGVLGMALFLEEMLRTRVGTKHAILLVAVSFVTLITGNMSFRDLGRVMVMVGATMTDDIG
  MGVTYLALLAAFKVRPTFAAGLLLRKLTSKELMMTTIGIVLLSQSTIPETILELTDALALGMMVLKMVRN
  MEKYQLAVTIMAILCVPNAVILQNAWKVSCTILAVVSVSPLLLTSSQQKTDWIPLALTIKGLNPTAIFLT
  TLSRTSKKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001474.2, NP_739585
• RefSeq ORF: 657
• MW: 23.8 kDa