nonstructural protein NS3 (NC_001474) Virus Tagged ORF Clone

CAT#: VC102549

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS3 [Dengue virus 2], codon optimized for human cell expression, NP_739587

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein NS3
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102549 represents NCBI reference of NP_739587 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGTGTTCTCTGGGACGTGCCTTCCCCCCCACCAATGGGAAAAGCAGAACTTGAAGATGGCGCAT
  ATAGAATTAAGCAGAAAGGAATCCTTGGGTACTCACAAATCGGGGCTGGCGTTTATAAGGAAGGGACCTT
  TCATACTATGTGGCACGTGACCCGGGGCGCTGTCCTTATGCACAAGGGCAAGAGAATCGAACCCTCTTGG
  GCAGACGTTAAGAAGGATCTTATTTCTTATGGGGGCGGCTGGAAACTGGAAGGCGAATGGAAGGAAGGGG
  AGGAGGTGCAGGTGCTGGCCTTGGAACCCGGTAAGAACCCACGAGCAGTGCAGACAAAACCCGGACTTTT
  TAAGACTAATGCTGGCACTATCGGCGCGGTGAGCCTTGATTTTTCCCCTGGCACATCTGGGTCCCCCATT
  ATTGACAAGAAAGGCAAAGTTGTTGGACTGTATGGAAACGGCGTTGTTACTCGGAGCGGGGCATATGTTT
  CTGCAATCGCGCAGACCGAGAAGTCAATCGAAGATAACCCCGAGATAGAGGATGACATTTTCCGAAAAAG
  GCGATTGACCATTATGGACTTGCATCCTGGAGCCGGCAAGACAAAGCGGTATCTCCCTGCTATAGTACGC
  GAAGCCATTAAACGAGGCCTTAGAACACTGATTCTGGCACCTACAAGAGTTGTTGCTGCAGAAATGGAGG
  AAGCCCTCAGGGGACTCCCTATAAGGTACCAGACACCCGCAATAAGAGCAGAGCACACAGGTCGGGAGAT
  CGTAGACCTTATGTGTCACGCCACCTTTACAATGAGATTGCTCTCCCCAGTACGGGTGCCGAACTACAAT
  CTTATCATTATGGATGAAGCGCATTTTACCGATCCGGCGTCAATTGCTGCAAGAGGATATATCAGCACAC
  GAGTGGAGATGGGTGAAGCGGCAGGGATTTTCATGACCGCCACCCCCCCAGGCTCTAGAGACCCTTTTCC
  CCAGTCCAACGCACCCATCATAGACGAGGAACGAGAAATTCCCGAGAGATCATGGAACAGCGGGCATGAA
  TGGGTGACCGATTTCAAGGGAAAAACAGTGTGGTTTGTCCCTAGCATCAAGGCCGGGAACGATATCGCAG
  CCTGCCTCCGGAAAAACGGCAAGAAGGTAATCCAACTGAGTAGGAAGACGTTTGATTCCGAATACGTGAA
  GACCCGAACTAATGACTGGGACTTCGTAGTGACGACAGATATTTCAGAGATGGGTGCCAATTTCAAAGCA
  GAACGAGTCATTGACCCTAGGAGATGCATGAAGCCGGTAATTCTCACCGATGGAGAAGAGAGGGTAATCC
  TGGCCGGGCCCATGCCCGTCACCCATAGCTCTGCGGCCCAGAGACGAGGCAGGATCGGACGGAATCCCAA
  GAACGAAAATGACCAATATATTTATATGGGAGAGCCTCTGGAGAACGACGAAGATTGTGCACACTGGAAA
  GAGGCCAAGATGCTGCTTGACAACATAAATACACCCGAGGGAATAATTCCAAGCATGTTCGAACCTGAGC
  GAGAAAAGGTTGACGCAATTGATGGCGAGTACCGCCTGAGAGGGGAGGCCAGGAAAACATTTGTGGACCT
  TATGCGCCGGGGAGATCTTCCCGTCTGGTTGGCCTACCGGGTAGCAGCTGAGGGCATCAACTATGCCGAT
  AGGAGATGGTGTTTCGACGGGGTCAAAAATAATCAGATACTGGAAGAAAACGTGGAGGTGGAGATTTGGA
  CAAAAGAGGGCGAGAGGAAAAAACTTAAACCTCGGTGGCTTGATGCAAGAATTTATTCCGATCCGTTGGC
  CTTGAAGGAGTTTAAAGAATTCGCCGCAGGGAGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102549 representing NP_739587
  Red=Cloning sites Green=Tags
  
  MAGVLWDVPSPPPMGKAELEDGAYRIKQKGILGYSQIGAGVYKEGTFHTMWHVTRGAVLMHKGKRIEPSW
  ADVKKDLISYGGGWKLEGEWKEGEEVQVLALEPGKNPRAVQTKPGLFKTNAGTIGAVSLDFSPGTSGSPI
  IDKKGKVVGLYGNGVVTRSGAYVSAIAQTEKSIEDNPEIEDDIFRKRRLTIMDLHPGAGKTKRYLPAIVR
  EAIKRGLRTLILAPTRVVAAEMEEALRGLPIRYQTPAIRAEHTGREIVDLMCHATFTMRLLSPVRVPNYN
  LIIMDEAHFTDPASIAARGYISTRVEMGEAAGIFMTATPPGSRDPFPQSNAPIIDEEREIPERSWNSGHE
  WVTDFKGKTVWFVPSIKAGNDIAACLRKNGKKVIQLSRKTFDSEYVKTRTNDWDFVVTTDISEMGANFKA
  ERVIDPRRCMKPVILTDGEERVILAGPMPVTHSSAAQRRGRIGRNPKNENDQYIYMGEPLENDEDCAHWK
  EAKMLLDNINTPEGIIPSMFEPEREKVDAIDGEYRLRGEARKTFVDLMRRGDLPVWLAYRVAAEGINYAD
  RRWCFDGVKNNQILEENVEVEIWTKEGERKKLKPRWLDARIYSDPLALKEFKEFAAGRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001474.2, NP_739587
• RefSeq ORF: 1857
• MW: 69.5 kDa