nonstructural protein NS4A (NC_001474) Virus Tagged ORF Clone

CAT#: VC102550

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4A [Dengue virus 2], codon optimized for human cell expression, NP_739588

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein NS4A
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102550 represents NCBI reference of NP_739588 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTCACTTTGAACCTGATTACTGAAATGGGTCGCCTTCCTACCTTTATGACACAAAAAGCTAGAG
  ACGCTTTGGACAACCTCGCCGTACTCCACACCGCGGAAGCTGGCGGACGAGCATATAATCACGCTCTCTC
  TGAACTGCCCGAAACTCTTGAAACACTGCTCCTCCTCACACTGCTTGCCACTGTTACCGGCGGCATCTTC
  CTTTTTCTTATGTCTGGTCGGGGAATCGGTAAAATGACACTTGGAATGTGTTGTATCATTACAGCCTCTA
  TACTCTTGTGGTATGCCCAGATTCAGCCGCATTGGATAGCTGCCTCTATTATACTTGAATTTTTTCTCAT
  CGTACTTCTTATACCTGAACCTGAAAAACAACGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102550 representing NP_739588
  Red=Cloning sites Green=Tags
  
  MSLTLNLITEMGRLPTFMTQKARDALDNLAVLHTAEAGGRAYNHALSELPETLETLLLLTLLATVTGGIF
  LFLMSGRGIGKMTLGMCCIITASILLWYAQIQPHWIAASIILEFFLIVLLIPEPEKQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 384 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001474.2, NP_739588
• RefSeq ORF: 384
• MW: 14.1 kDa