nonstructural protein NS4B (NC_001474) Virus Tagged ORF Clone

CAT#: VC102551

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS4B [Dengue virus 2], codon optimized for human cell expression, NP_739589

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein NS4B
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102551 represents NCBI reference of NP_739589 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGAAATGGGCTTCTTGGAGAAGACAAAAAAGGATCTGGGACTTGGATCTATCGCCACACAACAGC
  CCGAATCTAACATCCTCGATATTGATCTCCGGCCCGCATCCGCCTGGACCCTCTACGCCGTTGCCACCAC
  CTTTGTCACCCCTATGCTGCGCCACTCTATCGAAAACTCCTCCGTGAACGTTTCCCTGACCGCCATAGCT
  AACCAGGCCACCGTTCTGATGGGACTGGGGAAAGGCTGGCCTCTTTCAAAAATGGATATCGGTGTCCCAC
  TCCTTGCTATCGGATGCTATTCTCAGGTTAATCCCATTACTCTTACAGCTGCACTCTTTCTGCTCGTGGC
  ACACTACGCAATCATCGGCCCTGGACTTCAGGCAAAAGCCACTCGGGAAGCGCAAAAACGAGCTGCCGCA
  GGAATTATGAAAAACCCTACAGTCGACGGCATAACAGTTATAGATCTCGACCCTATCCCCTATGACCCTA
  AGTTTGAGAAGCAGCTTGGGCAAGTCATGCTCCTCGTCCTCTGTGTCACTCAGGTACTCATGATGAGAAC
  TACTTGGGCACTGTGTGAAGCCCTGACTCTTGCTACTGGACCCATTTCAACTCTGTGGGAAGGCAATCCC
  GGTAGGTTCTGGAACACAACCATCGCAGTCAGTATGGCCAATATATTCAGGGGCAGCTACCTCGCAGGCG
  CTGGACTCCTCTTTAGTATTATGAAAAACACAACGAACACACGAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102551 representing NP_739589
  Red=Cloning sites Green=Tags
  
  MNEMGFLEKTKKDLGLGSIATQQPESNILDIDLRPASAWTLYAVATTFVTPMLRHSIENSSVNVSLTAIA
  NQATVLMGLGKGWPLSKMDIGVPLLAIGCYSQVNPITLTAALFLLVAHYAIIGPGLQAKATREAQKRAAA
  GIMKNPTVDGITVIDLDPIPYDPKFEKQLGQVMLLVLCVTQVLMMRTTWALCEALTLATGPISTLWEGNP
  GRFWNTTIAVSMANIFRGSYLAGAGLLFSIMKNTTNTRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 747 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001474.2, NP_739589
• RefSeq ORF: 747
• MW: 26.9 kDa