capsid protein C (NC_001474) Virus Tagged ORF Clone

CAT#: VC102542

Myc-DDK-tagged ORF clone of viral ORF for capsid protein C [Dengue virus 2], codon optimized for human cell expression, NP_739591

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: capsid protein C
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102542 represents NCBI reference of NP_739591 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAACCAAAGAAAAAAGGCAAAAAATACACCCTTCAACATGCTCAAACGGGAACGCAATAGAGTCT
  CAACAGTTCAGCAACTTACAAAACGATTCAGCCTCGGTATGCTCCAGGGAAGAGGTCCACTTAAATTGTT
  TATGGCACTTGTCGCTTTCCTCCGCTTCCTCACCATCCCACCCACAGCAGGCATACTCAAAAGATGGGGA
  ACAATAAAGAAAAGCAAGGCAATCAACGTACTCCGCGGGTTTAGAAAAGAAATTGGAAGAATGCTTAATA
  TACTCAACAGACGCAGACGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102542 representing NP_739591
  Red=Cloning sites Green=Tags
  
  MNNQRKKAKNTPFNMLKRERNRVSTVQQLTKRFSLGMLQGRGPLKLFMALVAFLRFLTIPPTAGILKRWG
  TIKKSKAINVLRGFRKEIGRMLNILNRRRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 300 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001474.2, NP_739591
• RefSeq ORF: 300
• MW: 11.7 kDa