nonstructural protein NS2B (NC_001474) Virus Tagged ORF Clone

CAT#: VC102548

Myc-DDK-tagged ORF clone of viral ORF for nonstructural protein NS2B [Dengue virus 2], codon optimized for human cell expression, NP_739586

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: nonstructural protein NS2B
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102548 represents NCBI reference of NP_739586 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATGGCCCCTGAACGAAGCAATCATGGCAGTGGGCATGGTTTCTATTCTCGCCTCCAGCCTTTTGA
  AAAACGACATCCCCATGACCGGCCCCCTGGTAGCCGGAGGACTTCTGACAGTTTGCTACGTACTGACTGG
  TCGATCCGCAGACCTTGAACTTGAACGCGCCGCAGATGTGAAGTGGGAAGATCAGGCTGAAATTTCTGGC
  AGTTCCCCAATCTTGAGTATTACAATCAGCGAAGACGGATCTATGAGCATTAAAAACGAGGAAGAAGAAC
  AGACTTTGACTATATTGATTAGAACAGGGCTTTTGGTAATTTCAGGTCTCTTTCCAGTTTCTATCCCAAT
  AACCGCCGCAGCTTGGTATCTCTGGGAGGTTAAAAAACAAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102548 representing NP_739586
  Red=Cloning sites Green=Tags
  
  MSWPLNEAIMAVGMVSILASSLLKNDIPMTGPLVAGGLLTVCYVLTGRSADLELERAADVKWEDQAEISG
  SSPILSITISEDGSMSIKNEEEEQTLTILIRTGLLVISGLFPVSIPITAAAWYLWEVKKQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 393 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001474.2, NP_739586
• RefSeq ORF: 393
• MW: 14.2 kDa