membrane glycoprotein M (NC_001474) Virus Tagged ORF Clone

CAT#: VC102544

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein M [Dengue virus 2], codon optimized for human cell expression, NP_739592

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: membrane glycoprotein M
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102544 represents NCBI reference of NP_739592 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGTCGCTCTTGTTCCCCACGTTGGCATGGGACTCGAAACACGCACAGAAACTTGGATGTCCTCAG
  AAGGAGCATGGAAGCACGTTCAGAGAATTGAAACTTGGATCCTCAGACACCCCGGATTTACAATGATGGC
  CGCTATACTTGCATACACAATTGGCACAACACATTTCCAGAGAGCCCTGATATTTATTCTCCTCACCGCT
  GTAACCCCTTCAATGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102544 representing NP_739592
  Red=Cloning sites Green=Tags
  
  MSVALVPHVGMGLETRTETWMSSEGAWKHVQRIETWILRHPGFTMMAAILAYTIGTTHFQRALIFILLTA
  VTPSMT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 228 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001474.2, NP_739592
• RefSeq ORF: 228
• MW: 8.5 kDa