RNA-dependent RNA polymerase NS5 (NC_001474) Virus Tagged ORF Clone

CAT#: VC102552

Myc-DDK-tagged ORF clone of viral ORF for RNA-dependent RNA polymerase NS5 [Dengue virus 2], codon optimized for human cell expression, NP_739590

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNA-dependent RNA polymerase NS5
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102552 represents NCBI reference of NP_739590 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTACAGGAAATATAGGGGAGACTCTTGGTGAAAAGTGGAAGTCTAGACTGAACGCCCTTGGAAAAT
  CTGAGTTTCAGATCTACAAAAAGTCCGGAATCCAGGAGGTTGATCGAACACTCGCCAAGGAAGGAATCAA
  ACGGGGAGAGACCGACCATCATGCAGTATCACGCGGGTCTGCTAAGCTTCGCTGGTTCGTAGAACGAAAC
  ATGGTGACCCCCGAAGGCAAAGTCGTGGACCTTGGGTGCGGTCGGGGCGGCTGGAGCTATTACTGTGGAG
  GATTGAAAAACGTTCGGGAAGTGAAAGGCCTGACTAAGGGAGGACCAGGACACGAGGAACCTATTCCCAT
  GAGCACATACGGCTGGAACCTGGTACGACTTCAATCAGGTGTTGACGTGTTCTTCATCCCACCAGAGAAA
  TGTGATACTCTGCTCTGTGACATAGGAGAGTCTAGCCCCAATCCTACTGTTGAGGCCGGCAGAACCCTTC
  GCGTTTTGAACCTCGTGGAAAACTGGCTGAATAACAATACCCAATTTTGCATTAAAGTGCTTAATCCATA
  CATGCCCTCAGTAATAGAAAAGATGGAGGCCTTGCAACGAAAATACGGGGGCGCACTCGTAAGGAATCCT
  TTGTCTCGCAATTCAACCCACGAAATGTACTGGGTCTCCAACGCATCCGGAAACATTGTTAGTTCCGTGA
  ATATGATTTCCAGAATGCTGATTAACAGATTCACAATGCGGTACAAGAAAGCAACATACGAACCAGATGT
  AGACCTCGGCTCTGGAACTCGCAACATTGGCATTGAGTCTGAAATACCCAATCTGGACATTATCGGAAAA
  AGAATCGAGAAAATCAAACAAGAACATGAGACTTCCTGGCACTACGATCAGGACCATCCCTATAAAACCT
  GGGCTTACCACGGATCATATGAGACCAAGCAAACTGGATCCGCAAGTTCAATGGTTAACGGCGTAGTACG
  GCTTCTTACCAAACCATGGGATGTCGTTCCCATGGTGACCCAGATGGCAATGACTGATACAACACCATTC
  GGCCAGCAGCGAGTTTTTAAGGAGAAAGTCGACACACGCACTCAAGAGCCCAAAGAGGGTACAAAAAAGC
  TCATGAAAATAACAGCGGAGTGGTTGTGGAAAGAACTGGGCAAGAAAAAAACCCCTCGGATGTGTACTCG
  CGAAGAATTCACCCGGAAGGTGCGGAGTAATGCAGCCCTCGGGGCAATCTTTACTGACGAAAATAAATGG
  AAGAGCGCACGAGAAGCCGTCGAAGATTCACGATTCTGGGAACTGGTTGATAAAGAACGAAACTTGCATC
  TGGAAGGTAAATGCGAAACTTGCGTGTATAACATGATGGGGAAGCGCGAAAAAAAGCTGGGAGAATTCGG
  TAAAGCAAAGGGCAGCAGAGCTATTTGGTACATGTGGTTGGGCGCAAGGTTTCTGGAATTTGAGGCCCTG
  GGATTTTTGAATGAGGATCATTGGTTTAGTAGGGAGAATAGTTTGTCTGGCGTGGAGGGTGAAGGGCTTC
  ACAAGCTCGGCTACATCCTTAGAGATGTGAGCAAAAAAGAAGGAGGTGCTATGTACGCTGACGATACAGC
  TGGCTGGGACACACGCATTACACTCGAAGACCTGAAGAATGAAGAAATGGTAACAAATCACATGGAAGGA
  GAGCATAAAAAGCTCGCAGAGGCAATTTTTAAACTGACGTACCAAAATAAGGTGGTTCGAGTACAGCGCC
  CAACCCCACGAGGCACAGTAATGGATATAATTAGTCGCCGGGATCAGCGAGGATCAGGACAGGTTGGAAC
  ATACGGATTGAATACTTTCACCAACATGGAGGCACAACTGATCAGACAGATGGAAGGTGAAGGCGTTTTT
  AAGAGCATTCAGCACTTGACAATTACCGAGGAGATTGCGGTACAAAACTGGTTGGCCAGGGTCGGCAGGG
  AACGGCTTAGCAGAATGGCCATTTCAGGGGATGACTGTGTGGTTAAACCTCTCGACGATAGATTTGCTTC
  CGCTTTGACCGCTTTGAACGACATGGGTAAGATTCGCAAAGACATCCAACAGTGGGAACCAAGTCGGGGT
  TGGAATGATTGGACACAGGTGCCTTTCTGCTCTCATCATTTTCATGAGTTGATAATGAAAGATGGTCGCG
  TACTGGTTGTCCCATGTAGAAACCAGGACGAACTTATTGGAAGGGCCAGGATAAGCCAAGGAGCTGGATG
  GTCCCTGAGAGAGACTGCATGCCTCGGCAAATCATACGCCCAAATGTGGTCCCTCATGTATTTCCATAGG
  AGAGATCTCAGACTGGCAGCCAACGCCATTTGCTCTGCCGTTCCCTCACATTGGGTTCCGACAAGTAGGA
  CGACATGGTCTATTCACGCCAAACACGAGTGGATGACTACAGAGGATATGCTGACTGTGTGGAATCGCGT
  TTGGATTCAGGAAAACCCATGGATGGAGGATAAGACTCCCGTGGAGAGTTGGGAAGAAATCCCCTACTTG
  GGGAAAAGAGAAGATCAGTGGTGCGGTTCACTTATCGGCTTGACCTCTAGGGCCACTTGGGCCAAAAATA
  TTCAAGCTGCCATCAATCAGGTCAGGAGCCTGATTGGAAACGAGGAATACACAGATTACATGCCATCCAT
  GAAAAGATTTAGAAGGGAGGAGGAGGAGGCGGGAGTGTTGTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102552 representing NP_739590
  Red=Cloning sites Green=Tags
  
  MGTGNIGETLGEKWKSRLNALGKSEFQIYKKSGIQEVDRTLAKEGIKRGETDHHAVSRGSAKLRWFVERN
  MVTPEGKVVDLGCGRGGWSYYCGGLKNVREVKGLTKGGPGHEEPIPMSTYGWNLVRLQSGVDVFFIPPEK
  CDTLLCDIGESSPNPTVEAGRTLRVLNLVENWLNNNTQFCIKVLNPYMPSVIEKMEALQRKYGGALVRNP
  LSRNSTHEMYWVSNASGNIVSSVNMISRMLINRFTMRYKKATYEPDVDLGSGTRNIGIESEIPNLDIIGK
  RIEKIKQEHETSWHYDQDHPYKTWAYHGSYETKQTGSASSMVNGVVRLLTKPWDVVPMVTQMAMTDTTPF
  GQQRVFKEKVDTRTQEPKEGTKKLMKITAEWLWKELGKKKTPRMCTREEFTRKVRSNAALGAIFTDENKW
  KSAREAVEDSRFWELVDKERNLHLEGKCETCVYNMMGKREKKLGEFGKAKGSRAIWYMWLGARFLEFEAL
  GFLNEDHWFSRENSLSGVEGEGLHKLGYILRDVSKKEGGAMYADDTAGWDTRITLEDLKNEEMVTNHMEG
  EHKKLAEAIFKLTYQNKVVRVQRPTPRGTVMDIISRRDQRGSGQVGTYGLNTFTNMEAQLIRQMEGEGVF
  KSIQHLTITEEIAVQNWLARVGRERLSRMAISGDDCVVKPLDDRFASALTALNDMGKIRKDIQQWEPSRG
  WNDWTQVPFCSHHFHELIMKDGRVLVVPCRNQDELIGRARISQGAGWSLRETACLGKSYAQMWSLMYFHR
  RDLRLAANAICSAVPSHWVPTSRTTWSIHAKHEWMTTEDMLTVWNRVWIQENPWMEDKTPVESWEEIPYL
  GKREDQWCGSLIGLTSRATWAKNIQAAINQVRSLIGNEEYTDYMPSMKRFRREEEEAGVLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 2703 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001474.2, NP_739590
• RefSeq ORF: 2703
• MW: 103.4 kDa