envelope protein E (NC_001474) Virus Tagged ORF Clone

CAT#: VC102545

Myc-DDK-tagged ORF clone of viral ORF for envelope protein E [Dengue virus 2], codon optimized for human cell expression, NP_739583

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: envelope protein E
• Synonyms: DENV_gp1, Dengue, DENV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102545 represents NCBI reference of NP_739583 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGTATCGGAATGAGCAACAGGGATTTTGTTGAAGGCGTGTCAGGAGGATCTTGGGTCGACATTG
  TCCTCGAACATGGATCCTGTGTTACTACCATGGCAAAAAATAAACCCACCCTGGATTTTGAACTCATTAA
  AACCGAAGCCAAGCAGCCGGCCACACTGAGGAAATACTGCATTGAAGCTAAACTGACCAACACTACAACC
  GAATCTAGATGTCCAACCCAGGGCGAACCATCCCTCAACGAAGAACAAGATAAAAGATTCGTTTGTAAAC
  ATAGTATGGTTGATAGAGGATGGGGCAATGGATGCGGACTGTTCGGGAAAGGCGGGATTGTTACGTGTGC
  CATGTTCAGGTGCAAGAAGAACATGGAGGGAAAAGTGGTCCAACCAGAAAACCTTGAGTACACTATAGTA
  ATCACCCCCCATTCAGGCGAGGAACATGCCGTGGGAAACGACACGGGCAAACATGGAAAGGAGATCAAGA
  TTACCCCTCAATCTAGTATAACAGAGGCCGAACTGACCGGGTACGGGACCGTTACCATGGAATGTTCACC
  CCGGACCGGCCTGGACTTTAACGAGATGGTGCTCTTGCAGATGGAAAATAAAGCCTGGCTTGTGCATAGA
  CAATGGTTTCTGGACCTGCCACTGCCCTGGCTGCCTGGCGCTGATACGCAGGGCAGTAACTGGATTCAAA
  AGGAGACACTGGTTACCTTTAAGAACCCCCACGCGAAAAAGCAAGACGTTGTGGTGCTGGGCTCTCAGGA
  GGGGGCTATGCATACCGCACTGACAGGCGCGACAGAAATTCAGATGTCTTCTGGCAACCTCCTCTTTACT
  GGTCATTTGAAATGCAGATTGAGAATGGATAAACTGCAGTTGAAGGGCATGTCTTATAGTATGTGCACTG
  GCAAGTTTAAGGTCGTAAAAGAAATAGCTGAGACACAACATGGAACTATCGTCATCAGGGTGCAGTATGA
  AGGCGACGGCAGCCCATGCAAAATCCCCTTCGAAATTATGGATCTGGAAAAACGCCACGTGCTGGGCAGA
  CTTATCACCGTGAACCCCATCGTTACAGAGAAAGATTCACCCGTAAATATTGAGGCCGAGCCCCCATTTG
  GTGACTCATATATCATAATCGGCGTTGAGCCTGGACAGCTCAAACTGAACTGGTTTAAAAAAGGTTCCAG
  TATTGGCCAGATGTTCGAAACAACAATGCGCGGCGCCAAAAGGATGGCTATACTCGGAGATACAGCTTGG
  GACTTCGGGAGCCTTGGAGGTGTATTCACGTCTATTGGAAAAGCCCTGCATCAAGTATTTGGAGCAATTT
  ACGGCGCAGCTTTCAGCGGTGTTAGTTGGACAATGAAAATCCTGATCGGAGTAATTATTACCTGGATCGG
  AATGAACAGCAGATCTACAAGCCTGTCCGTCACACTCGTCCTTGTGGGAATCGTCACTCTTTATCTTGGT
  GTCATGGTGCAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102545 representing NP_739583
  Red=Cloning sites Green=Tags
  
  MRCIGMSNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIKTEAKQPATLRKYCIEAKLTNTTT
  ESRCPTQGEPSLNEEQDKRFVCKHSMVDRGWGNGCGLFGKGGIVTCAMFRCKKNMEGKVVQPENLEYTIV
  ITPHSGEEHAVGNDTGKHGKEIKITPQSSITEAELTGYGTVTMECSPRTGLDFNEMVLLQMENKAWLVHR
  QWFLDLPLPWLPGADTQGSNWIQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFT
  GHLKCRLRMDKLQLKGMSYSMCTGKFKVVKEIAETQHGTIVIRVQYEGDGSPCKIPFEIMDLEKRHVLGR
  LITVNPIVTEKDSPVNIEAEPPFGDSYIIIGVEPGQLKLNWFKKGSSIGQMFETTMRGAKRMAILGDTAW
  DFGSLGGVFTSIGKALHQVFGAIYGAAFSGVSWTMKILIGVIITWIGMNSRSTSLSVTLVLVGIVTLYLG
  VMVQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001474
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_001474.2, NP_739583
• RefSeq ORF: 1485 bp
• MW: 54.4 kDa