SARS-CoV-2 ORF3a Gene Tagged ORF Clone (Native Sequence)

CAT#: VC102567

Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], YP_009724391

Specifications

Product Data

• Tag: Myc-DDK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC202558 represents NCBI reference of YP_009724391
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  ATGGACCTCTTTATGCGCATTTTTACCATTGGCACCGTAACTCTCAAACAAGGTGAAATAAAGGACGCC
  ACCCCATCAGATTTTGTCCGAGCTACAGCAACCATCCCTATACAGGCATCCCTGCCTTTCGGCTGGCTC
  ATTGTTGGGGTCGCCCTTCTGGCCGTATTTCAATCCGCATCAAAAATTATCACTCTGAAGAAACGCTGG
  CAGCTGGCTCTGTCTAAAGGCGTGCACTTCGTCTGTAATCTCCTTCTCCTGTTTGTTACCGTCTACTCT
  CATCTTTTGCTCGTGGCTGCCGGCCTGGAAGCCCCATTTCTGTATCTCTATGCCCTCGTCTATTTTCTC
  CAATCAATTAATTTCGTTAGGATTATCATGCGCCTCTGGTTGTGCTGGAAATGCCGCTCCAAAAACCCC
  CTGCTCTATGACGCTAACTACTTCCTTTGCTGGCACACCAACTGCTATGATTACTGCATCCCCTATAAC
  TCTGTTACAAGTAGCATTGTGATCACATCTGGCGACGGCACAACTTCTCCAATCTCCGAGCACGATTAT
  CAAATTGGTGGCTATACTGAAAAATGGGAAAGTGGCGTAAAAGACTGCGTGGTGCTGCACTCCTATTTC
  ACATCCGATTATTACCAACTCTATTCAACCCAACTGTCAACTGATACAGGCGTCGAACACGTGACGTTC
  TTTATTTATAATAAGATTGTCGATGAACCTGAAGAACACGTACAGATCCACACAATCGACGGATCATCT
  GGCGTCGTCAACCCCGTAATGGAGCCCATCTACGACGAACCAACAACTACTACCTCTGTACCTCTG
  
  Protein Sequence

  >VC102558 representing YP_009724391
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRW
  QLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLEAPFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNP
  LLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYF
  TSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_045512
• ORF Size: 825 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NC_045512.2, YP_009724391
• RefSeq ORF: 825
• MW: 31.1 kDa