SARS-CoV-2 ORF3a Gene Tagged ORF (codon optimized)

CAT#: VC102558

Myc-DDK-tagged ORF clone for SARS-CoV-2 ORF3a protein [Severe acute respiratory syndrome coronavirus 2], codon optimized for human cell expression, YP_009724391

Specifications

Product Data

• Tag: Myc-DDK
• Symbol: ORF3a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102558 represents NCBI reference of YP_009724391 with codon optimized for human cell expression
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACCTCTTTATGCGCATTTTTACCATTGGCACCGTAACTCTCAAACAAGGTGAAATAAAGGACGCC
  ACCCCATCAGATTTTGTCCGAGCTACAGCAACCATCCCTATACAGGCATCCCTGCCTTTCGGCTGGCTC
  ATTGTTGGGGTCGCCCTTCTGGCCGTATTTCAATCCGCATCAAAAATTATCACTCTGAAGAAACGCTGG
  CAGCTGGCTCTGTCTAAAGGCGTGCACTTCGTCTGTAATCTCCTTCTCCTGTTTGTTACCGTCTACTCT
  CATCTTTTGCTCGTGGCTGCCGGCCTGGAAGCCCCATTTCTGTATCTCTATGCCCTCGTCTATTTTCTC
  CAATCAATTAATTTCGTTAGGATTATCATGCGCCTCTGGTTGTGCTGGAAATGCCGCTCCAAAAACCCC
  CTGCTCTATGACGCTAACTACTTCCTTTGCTGGCACACCAACTGCTATGATTACTGCATCCCCTATAAC
  TCTGTTACAAGTAGCATTGTGATCACATCTGGCGACGGCACAACTTCTCCAATCTCCGAGCACGATTAT
  CAAATTGGTGGCTATACTGAAAAATGGGAAAGTGGCGTAAAAGACTGCGTGGTGCTGCACTCCTATTTC
  ACATCCGATTATTACCAACTCTATTCAACCCAACTGTCAACTGATACAGGCGTCGAACACGTGACGTTC
  TTTATTTATAATAAGATTGTCGATGAACCTGAAGAACACGTACAGATCCACACAATCGACGGATCATCT
  GGCGTCGTCAACCCCGTAATGGAGCCCATCTACGACGAACCAACAACTACTACCTCTGTACCTCTG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >VC102558 representing YP_009724391
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRW
  QLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLEAPFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNP
  LLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYF
  TSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_045512
• ORF Size: 825 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NC_045512.2, YP_009724391
• RefSeq ORF: 825 bp
• MW: 31.1 kDa